One-pot pre-coated interface proximity extension assay for ultrasensitive co-detection of anti-SARS-CoV-2 antibodies and viral RNA

Yan, Sijia; Ahmad, Khalil; Warden, Antony R.; Ke, Yuqing; Maboyi, Nokuzola; Zhi, Xiao; Li, Yiyang

doi:10.1016/j.bios.2021.113535

Cited by 14 publications

(11 citation statements)

References 26 publications

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“…In addition, the current approach has several advantages compared to the alternative nanotechnology-based techniques, as detailed in Table 1 . 20 , 21 , 23 − 28 …”

Section: Resultsmentioning

confidence: 99%

“…In comparison, the current method benefits from application of both nanotechnology approaches and use of a new method for the synthesis and conjugation of probes to NPs, which are compared in timeline in Figure with more details. In addition, the current approach has several advantages compared to the alternative nanotechnology-based techniques, as detailed in Table . ,,− …”

Section: Resultsmentioning

confidence: 99%

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Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

2022

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A naked-eye (equipment-free), label-free (cost-effective), and RNA extraction-free (to speed up) method for SARS-CoV-2 (as a case study of RNA viruses) detection is developed. Here, the DNA is being used as a template for in situ formation of anisotropic gold nanoparticles (AuNPs) without any chemical modification or DNA labeling. In this study, synthesized AuNPs for the direct detection of N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2 are exploited. To this aim, antisense oligonucleotides (ASOs) with an extra poly guanine tail (G12) were designed. Thus, in the presence of its viral target RNA gene and ASOs@AuNPs-RNA hybridization, there was a red shift in its localized surface plasmon resonance (LSPR), and the intensity of the LSPR peak at 690 nm of throat swab samples was compared to the threshold cycle (Ct) of a reverse-transcriptase real-time polymerase chain reaction (RT-qPCR) (as a gold standard). Results suggested that the plasmonic biosensor can detect a very low amount of SARS-CoV-2 with a detection limit close to RT-qPCR. Simplicity of the new conjugation method with hybridization and annealing without amplification and denaturation steps enabled it to perform in a microfluidic paper-based analytical device.

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“…In addition, the current approach has several advantages compared to the alternative nanotechnology-based techniques, as detailed in Table 1 . 20 , 21 , 23 − 28 …”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Exploitation of N-Gene of SARS-CoV-2 to Develop a New Rapid Assay by ASOs@AuNPs

2022

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“…Also, we tested for SARS-CoV-2 antibodies using several kits selected by Korean Society for Laboratory Medicine and Korean Disease Control and Prevention Agency during spring of 2020. Several diagnostic measures have been developed since, with similar or better results [ 21 – 23 ]. Some include cost-efficient, faster detection methods, with some giving results within 45 min [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Prevalence of Antibodies to SARS-CoV-2 in 9954 Recruits in the Korean Army Training Center with the General Korean Population of Equivalent Age Between September and November, 2020

Mun¹,

Jang

Jeong³

et al. 2022

Med Sci Monit

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Background Understanding the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be a useful tool when studying spread of the disease. This study aimed to compare the prevalence of antibodies to SARS-CoV-2 in 9954 recruits in the Korean Army Training Center with the general Korean population age <30 years between September and November, 2020. Material/Methods At the Korean Army Training Center, samples were taken from 9954 men from September to November, 2020. Participants were randomly enlisted healthy adult men. The data were compared with 4,205,265 samples from the Korean general population. Men age <30 years were used, as this is similar to the age range of the military recruits. Results Among military recruits, 31 subjects (0.31%) were positive for the antibody, while the Korean male population had 3757 (0.09%) positive individuals. Among these 31 men, 13 were previously diagnosed by PCR, while 18 (58.06%) had no history related to the disease. Positive military recruits were mostly from 2 regional clusters. The first cluster was Daegu and Gyeongbuk areas (1.97% and 0.80%, respectively), which had an outbreak in March, 2020. The second cluster was Gyeonggi and Seoul, or capital areas (0.23% and 0.20%, respectively), which currently has high PCR positivity. Overall, seroprevalence was 3.49 times higher in study subjects. Conclusions The high seroprevalence of antibodies to SARS-CoV-2 between September and November 2020 in a densely populated military academy in Korea may have been an indicator for the resulting outbreak of COVID-19 in winter 2020–21, which highlights the importance of asymptomatic spread from the young and healthy to the general population.

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“…In addition, SARS-CoV-2 variants have emerged and caused higher transmission rates and stronger immune-escape responses [3] , [4] , [5] . Currently, RT-qPCR performed on nasopharyngeal and/or oropharyngeal swabs, remains the mainstream approach for SARS-CoV-2 diagnostic [6] , [7] , [8] , [9] . While it is sensitive and accurate, it requires well-trained personnel, highly purified samples, as well as expensive equipment such as a thermocycler, which limits its application in the under-equipped laboratories.…”

Section: Introductionmentioning

confidence: 99%