One-pot, direct glucose detection in human whole blood without using a dilution factor by a magnetic nanozyme with dual enzymatic activity

Gökçal, Burcu; Kip, Çiğdem; Tuncel, Alï

doi:10.1016/j.jallcom.2020.156012

Cited by 15 publications

(5 citation statements)

References 34 publications

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“…The activity GOx immobilized on H-CeO 2 @PDA NPs was determined by following the protocol given in Section S5 of the Supporting Information. , GOx binding onto H-CeO 2 @Ce6@PDA NPs was determined using a Bradford assay (Sigma-Aldrich, MO, USA, B6916).…”

Section: Methodsmentioning

confidence: 99%

In Vitro Synergistic Photodynamic, Photothermal, Chemodynamic, and Starvation Therapy Performance of Chlorin e6 Immobilized, Polydopamine-Coated Hollow, Porous Ceria-Based, Hypoxia-Tolerant Nanozymes Carrying a Cascade System

Süngü Akdogan,

Akbay Çetin,

Onur

et al. 2024

ACS Appl. Bio Mater.

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A synergistic therapy agent (STA) with photothermal, photodynamic, chemodynamic, and starvation therapy (PTT, PDT, CDT, and ST) functions was developed. Hollow, mesoporous, and nearly uniform CeO 2 nanoparticles (H-CeO 2 NPs) were synthesized using a staged shape templating sol−gel protocol. Chlorin e6 (Ce6) was adsorbed onto H-CeO 2 NPs, and a thin polydopamine (PDA) layer was formed on Ce6-adsorbed H-CeO 2 NPs. Glucose oxidase (GOx) was bound onto PDA-coated Ce6-adsorbed H-CeO 2 NPs to obtain the targeted STA (H-CeO 2 @Ce6@PDA@GOx NPs). A reversible photothermal conversion behavior with the temperature elevations up to 34 °C was observed by NIR laser irradiation at 808 nm. A cascade enzyme system based on immobilized GOx and intrinsic catalase-like activity of H-CeO 2 NPs was rendered on STA for enhancing the effectiveness of PDT by elevation of ROS generation and alleviation of hypoxia in a tumor microenvironment. Glucose-mediated generation of highly toxic hydroxyl radicals ( • OH) was evaluated for CDT. The effectiveness of PDT on glioblastoma T98G cells was markedly enhanced by O 2 generation started by the decomposition of glucose. A similar increase in cell death was also observed when ST and CDT functions were enhanced by photothermal action. The viability of T98G cells decreased to 10.6% by in vitro synergistic action including ST, CDT, PDT, and PTT without using any antitumor agent.

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Section: Methodsmentioning

confidence: 99%

In Vitro Synergistic Photodynamic, Photothermal, Chemodynamic, and Starvation Therapy Performance of Chlorin e6 Immobilized, Polydopamine-Coated Hollow, Porous Ceria-Based, Hypoxia-Tolerant Nanozymes Carrying a Cascade System

Süngü Akdogan,

Akbay Çetin,

Onur

et al. 2024

ACS Appl. Bio Mater.

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“…When the size of a material is conditioned to the nanometer level, a series of interesting properties emerge. In fact, apart from enzyme-like catalytic features, many materials also possess some attractive optical [105][106][107], magnetic [108][109][110], and electrical [111] properties or act as promising carriers [112][113][114] for biochemical analysis. These multiple properties of nanozymes offer biochemical sensing unparalleled benefits in practical applications.…”

Section: Multifunctional Nanozymesmentioning

confidence: 99%

Rational Design of Nanozymes Enables Advanced Biochemical Sensing

Liu

Niu

2022

Chemosensors

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In comparison with bioenzymes, nanozymes exhibit excellent robustness against extreme conditions, a low production cost, and easy-to-adjust properties, as well as potential versatility. These superiorities have attracted abundant interest in the last 15 years, to develop various nanozymes for applications including analytical sensing, environmental engineering, and biomedicine. In particular, for analytical sensing, a lot of nanozyme-involved principles and methods have been explored and applied to clinical diagnosis, environmental monitoring, food safety detection, and forensic analysis. Moreover, rational exploitation and use of nanozyme materials promote the performance of analytical methods. To highlight the latest progress in this attractive field, recent design concepts of nanozymes for advanced biochemical sensing are summarized. The development of single-atom nanozymes, self-cascade nanozymes, structurally biomimetic nanozymes, molecularly imprinted nanozymes, nanozymes breaking the pH limit, and multifunctional nanozymes is discussed in detail, to enhance detection sensitivity and selectivity, as well as expand application scenarios. Finally, some challenges and trends related to nanozyme-based sensors are reported, to satisfy the increasing needs of biochemical analysis with nanozymes.

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“…K m of SiO 2 @Au was much lower than that of horseradish peroxidase enzyme, indicating that SiO 2 @Au has a higher affinity for TMB than horseradish peroxidase (K m = 0.438 mM). Moreover, K m of SiO 2 @Au is lower than those Au NPs (K m = 0.123 mM), glucose-oxidase-conjugated Au-attached magnetic SiO 2 microsphere (K m = 0.208 mM), Au-NPs-decorated porous silica microsphere (K m = 0.523 mM), Prussian-blue-decorated latex nanoparticle (K m = 2.19 mM), MnO 2 nanoparticles (K m = 0.083 mM), and sulfatelatex-conjugated polyelectrolyte functionalized MnO 2 NPs (K m = 0.099 mM) [71][72][73][74][75].…”

Section: Effects Of Reaction Conditions On the Peroxidase-like Activity Of Sio 2 @Au Npsmentioning

confidence: 99%

“…Au-attached magnetic SiO2 microsphere (Km = 0.208 mM), Au-NPs-decorated porous silica microsphere (Km = 0.523 mM), Prussian-blue-decorated latex nanoparticle (Km = 2.19 mM), MnO2 nanoparticles (Km = 0.083 mM), and sulfate-latex-conjugated polyelectrolyte functionalized MnO2 NPs (Km = 0.099 mM) [71][72][73][74][75].…”

Section: Effects Of Reaction Conditions On the Peroxidase-like Activity Of Sio 2 @Au Npsmentioning

confidence: 99%

Synthesis of Finely Controllable Sizes of Au Nanoparticles on a Silica Template and Their Nanozyme Properties

Seong

Kim

et al. 2021

IJMS

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The precise synthesis of fine-sized nanoparticles is critical for realizing the advantages of nanoparticles for various applications. We developed a technique for preparing finely controllable sizes of gold nanoparticles (Au NPs) on a silica template, using the seed-mediated growth and interval dropping methods. These Au NPs, embedded on silica nanospheres (SiO2@Au NPs), possess peroxidase-like activity as nanozymes and have several advantages over other nanoparticle-based nanozymes. We confirmed their peroxidase activity; in addition, factors affecting the activity were investigated by varying the reaction conditions, such as concentrations of tetramethyl benzidine and H2O2, pH, particle amount, reaction time, and termination time. We found that SiO2@Au NPs are highly stable under long-term storage and reusable for five cycles. Our study, therefore, provides a novel method for controlling the properties of nanoparticles and for developing nanoparticle-based nanozymes.

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One-pot, direct glucose detection in human whole blood without using a dilution factor by a magnetic nanozyme with dual enzymatic activity

Cited by 15 publications

References 34 publications

In Vitro Synergistic Photodynamic, Photothermal, Chemodynamic, and Starvation Therapy Performance of Chlorin e6 Immobilized, Polydopamine-Coated Hollow, Porous Ceria-Based, Hypoxia-Tolerant Nanozymes Carrying a Cascade System

In Vitro Synergistic Photodynamic, Photothermal, Chemodynamic, and Starvation Therapy Performance of Chlorin e6 Immobilized, Polydopamine-Coated Hollow, Porous Ceria-Based, Hypoxia-Tolerant Nanozymes Carrying a Cascade System

Rational Design of Nanozymes Enables Advanced Biochemical Sensing

Synthesis of Finely Controllable Sizes of Au Nanoparticles on a Silica Template and Their Nanozyme Properties

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