One-pot biosynthesis of idoxuridine using nanostabilized lactic acid bacteria

Cappa, Valeria Alejandra; Trelles, Jorge A.

doi:10.1016/j.procbio.2017.07.028

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Even though a decrease of reaction times for the biosynthesis of DAC (data not shown) was not achieved, the entrapped biocatalysts showed a great stability at this temperature in contrast to the ionic biocatalyst (IDA- La NDT), which released enzyme after 6 h of incubation. This greater stability at higher temperatures is consistent with those results reported by Cappa and Trelles ( 2017 ). Besides, the entrapped biocatalyst showed improved stability after incubation in 40% of solvents such as acetonitrile and dimethyl sulfoxide with respect to the free biocatalyst.…”

Section: Discussionsupporting

confidence: 93%

“…As is well known, some bionanocomposites such as bentonite have shown improvements in the biocatalyst stability and its mechanical properties when they are incorporated into a matrix such as agarose or a hydrogel such as alginate (Cappa and Trelles 2017 ; De Benedetti et al 2015 ), usually without significantly affecting the biocatalyst activity. Stability improvements of the biocatalyst were evidenced in reusability and storage assays, in which the biocatalysts entrapped in matrices that included bentonite showed the best behavior.…”

Section: Discussionmentioning

confidence: 99%

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Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites

2020

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A novel IDA-LaNDT derivative was able to reach the highest productivity in the biosynthesis of a well-known antitumoral agent called decitabine. However, the combination of two simple and inexpensive techniques such as ionic absorption and gel entrapment with the incorporation of a bionanocomposite such as bentonite significantly improved the stability of this biocatalyst. These modifications allowed the enhancement of storage stability (for at least 18 months), reusability (400 h of successive batches without significant loss of its initial activity), and thermal and solvent stability with respect to the non-entrapped derivative. Moreover, reaction conditions were optimized by increasing the solubility of 5-aza by dilution with dimethylsulfoxide. Therefore, a scale-up of the bioprocess was assayed using the developed biocatalyst, obtaining 221 mg/L·h of DAC. Finally, green parameters were calculated using the nanostabilized biocatalyst, whose results indicated that it was able to biosynthesize DAC by a smooth, cheap, and environmentally friendly methodology.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Even though a decrease of reaction times for the biosynthesis of DAC (data not shown) was not achieved, the entrapped biocatalysts showed a great stability at this temperature in contrast to the ionic biocatalyst (IDA-LaNDT), which released enzyme after 6 h of incubation. This greater stability at higher temperatures is consistent with those results reported by Cappa and Trelles (Cappa and Trelles 2017). Besides, the entrapped biocatalyst showed improved stability after incubation in 40% of solvents such as acetonitrile and dimethyl sulfoxide with respect to the free biocatalyst.…”

Section: Discussionsupporting

confidence: 91%

“…As is well known, some bionanocomposites such as bentonite have shown improvements in the biocatalyst stability and its mechanical properties when they are incorporated into a matrix such as agarose or a hydrogel such as alginate (Cappa and Trelles 2017;De Benedetti et al 2015), usually without signi cantly affecting the biocatalyst activity. Stability improvements of the biocatalyst were evidenced in reusability and storage assays, in which the biocatalysts entrapped in matrices that included bentonite showed the best behavior.…”

Section: Discussionmentioning

confidence: 99%

Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites

Méndez

Trelles

Rivero

2020

Preprint

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A novel IDA-LaNDT derivative was able to reach the highest productivity in the biosynthesis of a well-known antitumoral agent called decitabine. However, the combination of two simple and inexpensive techniques such as ionic absorption and gel entrapment with the incorporation of a bionanocomposite such as bentonite significantly improved the stability of this biocatalyst. These modifications allowed the enhancement of storage stability (for at least 18 months), reusability (400 h of successive batches without significant loss of its initial activity), and thermal and solvent stability with respect to the non-entrapped derivative. Moreover, reaction conditions were optimized by increasing the solubility of 5-aza by dilution with dimethylsulfoxide. Therefore, a scale-up of the bioprocess was assayed using the developed biocatalyst, obtaining 221 mg/L.h of DAC. Finally, green parameters were calculated using the nanostabilized biocatalyst, whose results indicated that it was able to biosynthesize DAC by a smooth, cheap, and environmentally friendly methodology.

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