Decitabine bioproduction using a biocatalyst with improved stability by adding nanocomposites

Abstract

A novel IDA-LaNDT derivative was able to reach the highest productivity in the biosynthesis of a well-known antitumoral agent called decitabine. However, the combination of two simple and inexpensive techniques such as ionic absorption and gel entrapment with the incorporation of a bionanocomposite such as bentonite significantly improved the stability of this biocatalyst. These modifications allowed the enhancement of storage stability (for at least 18 months), reusability (400 h of successive batches without significant loss of its initial activity), and thermal and solvent stability with respect to the non-entrapped derivative. Moreover, reaction conditions were optimized by increasing the solubility of 5-aza by dilution with dimethylsulfoxide. Therefore, a scale-up of the bioprocess was assayed using the developed biocatalyst, obtaining 221 mg/L.h of DAC. Finally, green parameters were calculated using the nanostabilized biocatalyst, whose results indicated that it was able to biosynthesize DAC by a smooth, cheap, and environmentally friendly methodology.

Terapias no quirúrgicas que aceleran el movimiento ortodontico en humanos. Revisión de literatura.

“…Dentro de las terapias ortodónticas no se desean tratamientos largos, debido a los mayores riesgos de reabsorción radicular, descalcificación (El-Angbawi et al, 2015), lesiones de manchas blancas, caries dental, dolor, higiene oral subóptima, (Gkantidis et al, 2014) y recesión gingival, (Alwafi, 2017) entre otros efectos biológicos secundarios al tiempo extendido de la ortodoncia, al cual se ven expuestos los pacientes. (Cocios Arpi, Janina Fernanda;Trelles Méndez, Jessica Aracely;Jinez Zuñiga, Paulina Alexandra;Zapata Hidalgo, Christian David;& Ramos Montiel, 2021;Trelles Méndez et al, n.d.) En el ritmo acelerado de la vida actual, la necesidad de acortar el tiempo del tratamiento de ortodoncia es extremadamente importante. (Elkhadem & Sheba, 2015) Por ello, reducir la duración de la terapia activa puede reducir la incomodidad y la fatiga de los pacientes, en esa misma línea, mejorar el cumplimiento de las terapias.…”

Caninos impactados, diagnóstico e interpretación temprana. Revisión de literatura.

“…(Cocios Arpi, Janina Fernanda;Trelles Méndez, Jessica Aracely; Jinez Zuñiga, Paulina Alexandra;Zapata Hidalgo, Christian David;Ordoñez Pintado et al, 2021; Trelles Méndez et al, n.d.; Trelles Méndez, J. A., Toledo Jimenez, J.…”