One-pot and one-step colorimetric detection of aminopeptidase N activity based on gold nanoparticles-based supramolecular structure

Application of functional peptides in the electrochemical and optical biosensing of cancer biomarkers

“…In another work, our group proposed a one-pot and one-step colorimetric method for the detection of aminopeptidase N (APN) activity by making use of functional peptide-assisted supramolecular interaction, which is a predictive biomarker related to proliferation, invasion and metastasis of tumors. 79 As shown in Fig. 6C, the functional peptide probe contained a cleavage sequence (FGGF) that could act as both a recognition sequence of APN and a binding motif of cucurbit [8]uril (CB [8]), and a residual sequence (ELLC) to functionalize AuNPs through the Au-S bond.…”

The Peptide Functionalized Inorganic Nanoparticles for Cancer-Related Bioanalytical and Biomedical Applications

“…The peptide functionalized NPs have been extensively employed to construct biosensors/assays with various detection principles for detection of different analytes (some typical examples are included in Table 1 ) [ 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 ,…”

“…The LSPR of AuNP displays a red shift along with a visual color change from red to blue, while the dispersion state of AuNP is changed from monodispersing state to aggregating state. Various colorimetric assays based on peptide functionalized AuNPs have been developed for detection of a wide range of cancer-related species such as metallic ions [ 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ], small molecules [ 80 ], antigens/proteins [ 81 , 82 , 83 , 84 ], and enzymes [ 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 ].…”

“…It is found that abnormal enzyme activity is closely related to tumorigenesis and development, making enzymes such as kinases, proteases, and peptidases as important biomarkers for early cancer diagnosis and targets for therapeutic drug development. The colorimetric assays based on peptide functionalized AuNPs can be employed to determine enzyme activity and act as a screening inhibitor of the enzyme, when the AuNPs are functionalized by peptide substrate of enzyme [ 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 ]. As early as 2006, Wang and colleagues demonstrated that the interactions of biotinylated peptide substrate functionalized AuNPs and avidin-modified AuNPs could be employed to develop a colorimetric assay for the evaluation of kinase activity and inhibition [ 85 ].…”

“…In this case, using γ-biotin-ATP as a cosubstrate, the kinase reaction results in the biotinylation of the peptide substrate on AuNPs. Mao and colleagues proposed a one-pot and one-step colorimetric sensing method for detecting activity of aminopeptidase N (APN) based on a peptide (NH 2 -FGGFELLC-Ac) functionalized AuNPs/cucurbit[8]uril (pep-AuNPs/CB[8]) supramolecular structure which was formed by the crosslinking of pep-AuNPs with CB[8] [ 95 ]. In the presence of APN, the pep-AuNPs/CB[8] supramolecular structure is disassembled because the peptide will be hydrolyzed by APN.…”