One novel strawberry MADS-box transcription factor FaMADS1a acts as a negative regulator in fruit ripening

Lu, Wenjing; Chen, Jingxin; Ren, Xingchen; Yuan, Jiajia; Han, Xueyuan; Mao, Linchun; Ying, Tiejin; Luo, Zisheng

doi:10.1016/j.scienta.2017.09.042

Cited by 33 publications

(32 citation statements)

References 38 publications

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“…Albeit, MADS-box TFs are able to change the anthocyanin content and color of the fruit (Seymour et al, 2011), the series of genes regulated by MADS-box TFs are still unrevealed. Interestingly, FaMADS1a is expressed with delayed ripening phenotype, and its expression is induced by auxin and suppressed by ABA, concomitantly speeding up the ripening process (Lu et al, 2018). In F. chiloensis two MADS-box genes ( FcMADS1 and FcMADS2 ) have been identified, and both genes are expressed during fruit ripening, but also highly expressed in flower tissues (Pimentel et al, 2010).…”

Section: Transcription Factors and Fruit Ripeningmentioning

confidence: 99%

Molecular Events Occurring During Softening of Strawberry Fruit

2019

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Changes in fruit texture taking place during ripening, described as softening, are mainly due to alterations in structure and/or composition of the cell wall. Several non-covalent interactions between the three carbohydrate polymers of the cell wall, cellulose, pectins and hemicellulose, and many structural proteins and ions, enable a complex structure. During softening, the disassembly of the cell wall structure takes place, mediated by a complete set of cell wall degrading enzymes or proteins. Softening is a coordinated event that requires the orchestrated participation of a wide variety of proteins. Plant hormones and a set of transcription factors are the organizers of this multi-protein effort. Strawberry is a non climacteric fruit that softens intensively during the last stages of development. The Chilean strawberry fruit ( Fragaria chiloensis ), the maternal relative of the commercial strawberry ( F. × ananassa ), softens even faster than commercial strawberry. Softening of the Chilean strawberry fruit has been studied at different levels: changes in cell wall polymers, activity of cell wall degrading enzymes and transcriptional changes of their genes, providing a general view of the complex process. The search for the ‘orchestra director’ that could coordinate softening events in strawberry fruit has been focussed on hormones like ABA and auxins, and more precisely the relation ABA/AUX. These hormones regulate the expression of many cell wall degrading enzyme genes, and this massive transcriptional change that takes place involves the participation of key transcriptional factors (TF). This review provides an update of the present knowledge regarding the softening of strawberry fruit. Nevertheless, the entire softening process is still under active research especially for the great influence of texture on fruit quality and its high impact on fruit shelf life, and therefore it is expected that new and promising information will illuminate the field in the near future.

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Section: Transcription Factors and Fruit Ripeningmentioning

confidence: 99%

Molecular Events Occurring During Softening of Strawberry Fruit

2019

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“…Anthocyanins are synthesized via the flavonoid pathway and are regulated by many structural genes, including CHS, CHI, DFR, F3H, UFGT, and ANS (Hichri et al, 2011). Moreover, some transcription factors (TFs) affect the synthesis of anthocyanins by binding to the promoter regions of structural genes, such as bZIP, MYB, bHLH, MADS-box, and WD (An et al, 2017;Lu et al, 2018;Jian et al, 2019). Among them, the regulatory function of the MBW (MYB-bHLH-WD) protein complex consisting of MYB, bHLH, and WD in anthocyanin synthesis is well stablished (Feng et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Regulatory Mechanism of Color Diversity in Camellia japonica Petals by Integrative Transcriptome and Metabolome Analysis

Xu²,

Zheng

et al. 2021

Front. Plant Sci.

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Camellia japonica petals are colorful, rich in anthocyanins, and possess important ornamental, edible, and medicinal value. However, the regulatory mechanism of anthocyanin accumulation in C. japonica is still unclear. In this study, an integrative analysis of the metabolome and transcriptome was conducted in five C. japonica cultivars with different petal colors. Overall, a total of 187 flavonoids were identified (including 25 anthocyanins), and 11 anthocyanins were markedly differentially accumulated among these petals, contributing to the different petal colors in C. japonica. Moreover, cyanidin-3-O-(6″-O-malonyl) glucoside was confirmed as the main contributor to the red petal phenotype, while cyanidin-3-O-rutinoside, peonidin-3-O-glucoside, cyanidin-3-O-glucoside, and pelargonidin-3-O-glucoside were responsible for the deep coloration of the C. japonica petals. Furthermore, a total of 12,531 differentially expressed genes (DEGs) and overlapping DEGs (634 DEGs) were identified by RNA sequencing, and the correlation between the expression level of the DEGs and the anthocyanin content was explored. The candidate genes regulating anthocyanin accumulation in the C. japonica petals were identified and included 37 structural genes (especially CjANS and Cj4CL), 18 keys differentially expressed transcription factors (such as GATA, MYB, bHLH, WRKY, and NAC), and 16 other regulators (mainly including transporter proteins, zinc-finger proteins, and others). Our results provide new insights for elucidating the function of anthocyanins in C. japonica petal color expression.

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“…Moreover, some other TFs such as the MYB-bHLH-WD (MBW) complex, B-box, bZIP, MYC, NAC, WRKY, bHLH, MADS-box, and WD could also coordinate anthocyanin biosynthesis initiation by binding to the promoter regions of structural genes ( Xu et al, 2015 ; Zhou et al, 2015 ; An et al, 2017 ; Lloyd et al, 2017 ; Lu et al, 2018 ; Fang et al, 2019 ; Jiang et al, 2019 ). For example, Arabidopsis bHLH TFs (GL3, TT8, and EGL3) and WD40 repeat protein TTG1 regulate anthocyanin biosynthetic gene expressions ( Gonzalez et al, 2008 ; Gerats and Strommer, 2009 ; Saito et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, anthocyanin biosynthesis in petunia petal cells is controlled by the MBW complex, comprising subgroups of MYB TF (PhAN2 or PhAN4) and bHLH TF (PhAN1 or PhJAF13), as well as the WD40 regulator PhAN11 ( Quattrocchio et al, 2006 ). Strawberry FaMADS1a played a negative role in anthocyanin accumulation via repressing expression of FaPAL6 , FaCHS , FaDFR , and FaANS ( Lu et al, 2018 ). Furthermore, apple B-box zinc finger protein MdBBX20 promotes anthocyanin accumulation in response to ultraviolet-B radiation and low temperature ( Fang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Identifies Key Regulatory Genes Involved in Anthocyanin Metabolism During Flower Development in Lycoris radiata

et al. 2021

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Lycoris is used as a garden flower due to the colorful and its special flowers. Floral coloration of Lycoris is a vital trait that is mainly regulated via the anthocyanin biosynthetic pathway. In this study, we performed a comparative transcriptome analysis of Lycoris radiata petals at four different flower development stages. A total of 38,798 differentially expressed genes (DEGs) were identified by RNA sequencing, and the correlation between the expression level of the DEGs and the anthocyanin content was explored. The identified DEGs are significantly categorized into ‘flavonoid biosynthesis,’ ‘phenylpropanoid biosynthesis,’ ‘Tropane, piperidine and pyridine alkaloid biosynthesis,’ ‘terpenoid backbone biosynthesis’ and ‘plant hormone signal transduction’ by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The candidate genes involved in anthocyanin accumulation in L. radiata petals during flower development stages were also identified, which included 56 structural genes (especially LrDFR1 and LrFLS) as well as 27 key transcription factor DEGs (such as C3H, GATA, MYB, and NAC). In addition, a key structural gene namely LrDFR1 of anthocyanin biosynthesis pathway was identified as a hub gene in anthocyanin metabolism network. During flower development stages, the expression level of LrDFR1 was positively correlated with the anthocyanin content. Subcellular localization revealed that LrDFR1 is majorly localized in the nucleus, cytoplasm and cell membrane. Overexpression of LrDFR1 increased the anthocyanin accumulation in tobacco leaves and Lycoris petals, suggesting that LrDFR1 acts as a positively regulator of anthocyanin biosynthesis. Our results provide new insights for elucidating the function of anthocyanins in L. radiata petal coloring during flower development.

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One novel strawberry MADS-box transcription factor FaMADS1a acts as a negative regulator in fruit ripening

Cited by 33 publications

References 38 publications

Molecular Events Occurring During Softening of Strawberry Fruit

Molecular Events Occurring During Softening of Strawberry Fruit

Unraveling the Regulatory Mechanism of Color Diversity in Camellia japonica Petals by Integrative Transcriptome and Metabolome Analysis

Comparative Transcriptome Analysis Identifies Key Regulatory Genes Involved in Anthocyanin Metabolism During Flower Development in Lycoris radiata

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