One Novel Multiple-Target Plasmid Reference Molecule Targeting Eight Genetically Modified Canola Events for Genetically Modified Canola Detection

Li, Zhuqing; Li, Xiang; Wang, Canhua; Gao, Song; Pi, Liqun; Zheng, Lan; Zhang, Dabing; Yang, Litao

doi:10.1021/acs.jafc.7b02453

Cited by 14 publications

(5 citation statements)

References 32 publications

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“…To investigate the changes in transgenic fragments during the extraction of transgenic soybean oil, Xia et al ( 2021) amplified the lectin and cp4epsps gene fragments by traditional PCR. Traditional PCR can also detect whether the newly constructed plasmid molecules can be used as transcriptional templates for transgenic rapeseed and endogenous genes (Li et al, 2017). In summary, traditional PCR is mainly applied to amplify the DNA of the samples in transgenic determination.…”

Section: Traditional Pcrmentioning

confidence: 99%

A review on the identification of transgenic oilseeds and oils

Pan

Xing

Zhang

et al. 2023

Journal of Food Science

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Transgenic technology can increase the quantity and quality of vegetable oils worldwide. However, people are skeptical about the safety of transgenic oil‐bearing crops and the oils they produce. In order to protect consumers’ rights and avoid transgenic oils being adulterated or labeled as nontransgenic oils, the transgenic detection technology of oilseeds and oils needs careful consideration. This paper first summarized the current research status of transgenic technologies implemented at oil‐bearing crops. Then, an inspection process was proposed to detect a large number of samples to be the subject rapidly, and various inspection strategies for transgenic oilseeds and oils were summarized according to the process sequence. The detection indicators included oil content, fatty acid, triglyceride, tocopherol, and nucleic acid. The detection technologies involved chromatography, spectroscopy, nuclear magnetic resonance, and polymerase chain reaction. It is hoped that this article can provide crucial technical reference and support for staff engaging in the supervision of transgenic food and for researchers developing fast and efficient monitoring methods in the future.

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Section: Traditional Pcrmentioning

confidence: 99%

A review on the identification of transgenic oilseeds and oils

Pan

Xing

Zhang

et al. 2023

Journal of Food Science

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“…Compared with matrix materials, recombinant plasmids are linear or circular molecules containing the target gene, can be easily prepared in large quantities, and are not limited by the source of raw materials. , In addition, recombinant plasmids can efficiently and quickly obtain unlimited amounts with high stability in complex transportation environments . The special properties make them an excellent material for NRMs of detection method. − …”

Section: Introductionmentioning

confidence: 99%

Development of DNA Reference Materials of Citrus Huanglongbing Candidatus Liberibacter asiaticus

Chen,

Li,

et al. 2024

ACS Agric. Sci. Technol.

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Citrus Huanglongbing (HLB) is a devastating disease within the Citrus industry. Candidatus Liberibacter asiaticus (CLas) is one of the most prevalent HLB-associated strains that has not been cultured in vitro. To ensure the accuracy and comparability of the molecular diagnostic method for HLB detection, certified reference materials urgently need to be developed for CLas detection. Here, we developed a series of DNA reference materials of CLas using 16S rDNA as the target gene and the SAND gene as the Citrus reference gene. The 16S rDNA gene fragment cloned by the NCBI sequence and Citrus DNA extracted by healthy Citrus leaves are thoroughly mixed for preparation. Droplet digital PCR (ddPCR) was used as an accurate quantification method for 16S rDNA, and the SAND was established and optimized through this study. Nine laboratories collaborated in determining these two parameters, and the homogeneity and stability were adequate. The quantification results demonstrated that the copy number certified values and expanded uncertainty of 16S rDNA and SAND in the high-concentration reference material were (3.86 ± 0.34) × 10 3 and (4.43 ± 0.39) × 10 3 cp/μL, respectively. The copy number certified values and expanded uncertainty of 16S rDNA and SAND in the low-concentration reference material were (3.98 ± 0.36) × 10 2 and (4.34 ± 0.37) × 10 3 cp/μL, respectively. In addition, this certified reference material will provide reliable quality control for detecting CLas.

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“…The disadvantages of matrix-based CRMs in GMO quantitative analysis include the narrow dynamic range of quantification, multiple and complex preparation procedures, high cost, and difficulty in obtaining the homogeneous candidate [ 15 , 16 ]. Compared with matrix-based CRMs, plasmid-DNA-based CRMs are good substitutes in GMO analysis since they have a well-characterized transgene sequence and copy number, and they can be easily maintained in and produced from bacterium stocks [ 17 , 18 , 19 , 20 , 21 ]. In the detection of foodborne bacteria and viruses, plasmid-DNA-based CRMs are also widely used instead of inactivated bacteria and viruses [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810

Meng

Wang

Guo

et al. 2022

Foods

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Certified reference materials (CRMs) is one of the critical requirements in a quantitative analytical method, such as in the quantification of genetically modified (GM) contents in food/feed products. Plasmid-DNA-based CRMs are becoming essential in GM content quantification. Herein, we report the construction of one plasmid DNA calibrant, pMON810, for the quantification of the GM maize event MON810 which is commercially planted and used for food/feeds worldwide, and the collaborative ring trial was used to validate its applicability. pMON10 was proven to have high specificity for the MON810 event. The limit of detection (LOD) and limit of quantification (LOQ) of real-time PCR assays of MON810 event and maize endogenous gene using pMON810 as calibrant was 2 copies/μL and 5 copies/μL, respectively. A total of eight laboratories participated in the ring trial and returned valid test results. Each sample was performed with three repeats and three parallels in each repeat. Statistical analysis of the ring trial results showed that pMON810 as a calibrant had high PCR efficiency (ranging from 0.885 to 1.008) and good linearity (ranging from 0.9933 to 0.9997) in MON810 and endogenous gene real-time PCR assays. The bias between the test values and true values ranged from 4.60 to 20.00% in the quantification of five blind samples. These results indicate that pMON810 is suitable for use as a calibrant for the quantification of MON810 events in routine lab analysis or to evaluate detection methods for MON810, as well as being used as a substitute for the matrix-based CRM of MON810.

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One Novel Multiple-Target Plasmid Reference Molecule Targeting Eight Genetically Modified Canola Events for Genetically Modified Canola Detection

Cited by 14 publications

References 32 publications

A review on the identification of transgenic oilseeds and oils

A review on the identification of transgenic oilseeds and oils

Development of DNA Reference Materials of Citrus Huanglongbing Candidatus Liberibacter asiaticus

Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810

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