Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810

Meng, Yanan; Wang, Shu; Guo, Jinchao; Liu, Yang

doi:10.3390/foods11111538

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Qualitative tests can be used to distinguish between approved and unauthorized material or for certifying the purity of the material; quantitative tests can be used for labelling. Testing can play a role in GMO safety assessment and risk management as a tracing tool [7] or for traceability of GM and derivatives after consumption [8]. Currently, transgene detection at the nucleic acid level is based on four different detection strategies, namely, element screening, gene-specific PCR of the target, construct-specific PCR, and event-specific PCR.…”

Section: Introductionmentioning

confidence: 99%

The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the <em>cry1Ac</em> Gene with Concomitant Enhancement of Its Insecticidal Activity

Huang,

Zhang,

et al. 2024

Preprint

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The structure and expression of exogenous genes in transgenic crops are critical for the target traits. R7569 was a mutant event identified in this study with deletion at the 3' end of cry1Ac gene compared to the transgenic insect-resistant cotton MON531 event with commercial application. R7569 has the same exogenous insertion structure as MON531, but with a deletion in the 3' end of the cry1Ac gene and the terminator region. R7569 has a truncated cry1Ac gene with the length of 2,554 bp, encoding 881 amino acids. The transcription termination site was mainly concentrated downstream of the truncated position and extended 160-270 bp from the truncated position using rapid-amplification of cDNA ends (RACE). The transcript levels of cry1Ac genes of R7569 and MON531 decreased gradually at seedling, bud and bell stages, and the transcript levels of cry1Ac genes of R7569 were significantly higher than those of MON531 in seedling and bud stages, but there was no significant difference in the boll stage. The content of Cry1Ac protein in R7569 gradually decreased with the seedling, bud and boll stage, and the content of Cry1Ac protein in all three periods was higher than that of MON531. The insect resistance assay showed that the resistance levels of R7569 and MON531 were both at the high level, and the corrected mortality rate against bollworms was 99.5% and 95.2%, respectively, and there was no significant difference between them. The LC50 value of R7569 was 0.732ng/g dw, with a slope of 1.654 indicating a high level of resistance to bollworm. In this study, for the first time, we found a partial deletion of the target gene in commercially applied transgenic crops, and the partial deletion of the 3' end of the cry1Ac gene retained a better transcription, expression level and insecticidal activity, which can provide a specific case for the safety evaluation of transgenic crops.

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Section: Introductionmentioning

confidence: 99%

The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the <em>cry1Ac</em> Gene with Concomitant Enhancement of Its Insecticidal Activity

Huang,

Zhang,

et al. 2024

Preprint

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show abstract

Development and verification of new reference plasmid (pUC_GM-SB) for the DNA-based detection of genetically modified sugar beet H7-1

Suh,

Kim,

Kim

et al. 2024

Food Sci Biotechnol

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Deletion of the 3′ End of the Introduced cry1Ac Gene Retains the Insecticidal Activity in Transgenic Cotton

Huang,

Zhang,

et al. 2024

Agronomy

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The structure and expression of exogenous genes in transgenic crops are critical for the target traits. R7569 has the same exogenous insertion structure as the transgenic insect-resistant cotton MON531 but with a deletion in the 3′ end of the cry1Ac gene and the terminator region. Thus, in the present study, transcription, expression, and insecticidal activity assays were conducted to determine the function of the truncated cry1Ac gene. R7569 has a truncated cry1Ac gene with a length of 2554 bp encoding 881 amino acids, and the transcription termination site was mainly concentrated downstream of the truncated position and extended 160-270 bp from the truncated position using rapid amplification of cDNA ends (RACE). The transcript levels of the cry1Ac gene in R7569 were significantly higher than those of MON531 implants, except for during the boll stage. The content of the Cry1Ac protein in R7569 was higher than that of MON531 in the cotton leaf in all three periods. The corrected mortality rates of R7569 and MON531 against bollworms were 93.09% and 88.83%, respectively. The LC50 value of R7569 was 0.732 ng/g (dw), indicating a high level of resistance to bollworm. In this study, for the first time, we found a partial deletion of the target gene in commercially applied transgenic crops, and the partial deletion of the 3′ end of the cry1Ac gene retained a better transcription, expression level, and insecticidal activity, which can provide a specific case for the safety evaluation of transgenic crops.

show abstract

Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810

Cited by 3 publications

References 25 publications

The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the <em>cry1Ac</em> Gene with Concomitant Enhancement of Its Insecticidal Activity

The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the <em>cry1Ac</em> Gene with Concomitant Enhancement of Its Insecticidal Activity

Development and verification of new reference plasmid (pUC_GM-SB) for the DNA-based detection of genetically modified sugar beet H7-1

Deletion of the 3′ End of the Introduced cry1Ac Gene Retains the Insecticidal Activity in Transgenic Cotton

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