One new flavanocoumarin from the thorns of Gleditsia sinensis

Abstract

One new flavanocoumarin (1), as well as six known flavonoids (2-7), was isolated from the ethyl acetate extract of the thorns of Gleditsia sinensis. The structures of these compounds were elucidated by extensive spectroscopic measurements and comparison with data reported in literatures. Cytotoxic activities of compounds 1-6 were evaluated against human liver cancer SK-hep-1 cells in vitro by the MTT method, with compound 1 displaying moderate activity (IC of 62.53 μM). Furthermore, compound 1 could increase the number of apoptosis cells in a concentration-dependent manner.

Comprehensive profiling of chemical composition of Gleditsiae spina using ultra‐high‐performance liquid chromatography coupled with electrospray ionization quadrupole time‐of‐flight mass spectrometry

“…Fragment ions at m/z 151.0036 and 151.0404 were formed by RDA fragmentation. Therefore, compound 103 was identified as padmatin 4,32 . Compound 104 yielded a precursor ion at m/z 273.0768 ([M‐H] − , C 15 H 14 O 5 ), which was identified as apiforol by the fragment ions at m/z 135.0045 and 137.0241.…”

“…GS has detumescence, detoxification, purulence, and insecticidal effects and is helpful in treating the initial onset of carbuncle, expelling pus, and as a topical treatment of scabies and leprosy. The chemical constituents of GS include flavonoids, lignans, and other phenolic acids 2–5 . Research shows that GS contains many bioactive phytochemical components attributed to its variety of medicinal values, including anticancer, anti‐inflammatory, antiatherogenic, antimicrobial, antiallergic, and antivirus activities 6–12 …”

“…The chemical constituents of GS include flavonoids, lignans, and other phenolic acids. [2][3][4][5] Research shows that GS contains many bioactive phytochemical components attributed to its variety of medicinal values, including anticancer, anti-inflammatory, antiatherogenic, antimicrobial, antiallergic, and antivirus activities. [6][7][8][9][10][11][12] Considerable research studies have focused on the extraction and separation of compounds from GS.…”

Application of choline chloride deep eutectic solvents and high‐speed counter‐current chromatography to the extraction and purification of flavonoids from the thorns of Gleditsia sinensis Lam

“…14,15 Previous research has identified the active compounds from the thorns of G. sinensis mainly as flavonoids, which showed antimicrobial, antiviral, anticancer, and anticoagulant activities. 16,17 Increasingly, pharmacological assays are used to investigate the bioactivities of these characteristic metabolites, which have been applied for more and more medicines to treat diseases. Correspondingly, various extraction methods for flavonoids from the thorns of G. sinensis have been reported, such as reflux extraction, ultrasonic extraction, and so on.…”

“…Correspondingly, various extraction methods for flavonoids from the thorns of G. sinensis have been reported, such as reflux extraction, ultrasonic extraction, and so on. [16][17][18][19] However, solvents of these methods are usually organic solvents, such as methanol, ethanol, ethyl acetate and acetone, toxicities of which is usually non-negligible and usually led to environmental pollution. So, the green production of flavonoids from the thorns of G. sinensis is valuable.…”