One Example on How Colloidal Nano- and Microparticles Could Contribute to Medicine

Peteiro-Cartelle, Javier; Rodríguez-Pedreira, Montserrat; Zhang, Feng; Rivera-Gil, Pilar; Mercato, Loretta L. del; Parak, Wolfgang J.

doi:10.2217/nnm.09.84

Cited by 42 publications

(31 citation statements)

References 94 publications

(86 reference statements)

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“…There is a multitude of reports about the interaction of colloidal nanoparticles (NPs) with mammalian cells [1], as this topic is important for analyzing intended (e.g., medical applications [2–4]) and non-intended (e.g., contamination [5–7]) exposure of NPs to humans. However, there is a great number of available NPs made of many different materials [8–10] with a wide range of different functionalities, cf.…”

Section: Introductionmentioning

confidence: 99%

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

Nazarenus

Zhang

Soliman

et al. 2014

Beilstein J. Nanotechnol.

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SummaryThe interfacing of colloidal nanoparticles with mammalian cells is now well into its second decade. In this review our goal is to highlight the more generally accepted concepts that we have gleaned from nearly twenty years of research. While details of these complex interactions strongly depend, amongst others, upon the specific properties of the nanoparticles used, the cell type, and their environmental conditions, a number of fundamental principles exist, which are outlined in this review.

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Section: Introductionmentioning

confidence: 99%

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

Nazarenus

Zhang

Soliman

et al. 2014

Beilstein J. Nanotechnol.

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136

123

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“…Among several drug carriers currently under investigation for improved drug efficacy and reduced toxicity, nanoparticles hold the greatest promise [10,11]. Polymeric micelles, which are formed by the self-assembly of amphiphilic polymers, have a segregated corecorona architecture which makes them a unique class of colloidal drug carriers [12,13].…”

Section: Introductionmentioning

confidence: 99%

Tailoring the efficacy of nimodipine drug delivery using nanocarriers based on A2B miktoarm star polymers

Soliman¹,

Sharma²,

Choi³

et al. 2010

Biomaterials

117

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a b s t r a c tWe report a nanocarrier based on A 2 B type miktoarm polymers (A ¼ polyethylene glycol (PEG); B ¼ polycaprolactone (PCL)) for nimodipine (NIM), a hydrophobic drug with very poor aqueous solubility that is commonly prescribed for the prevention and treatment of delayed ischemic neurological disorders. The A 2 B star polymers were constructed on a core with orthogonal functionalities that facilitated the performance of "click" chemistry followed by ring-opening polymerization. These star polymers assemble into spherical micelles into which NIM can be easily loaded by the co-solvent evaporation method. The micelles obtained from the star polymer PEG775 2 ePCL5800 showed NIM encapsulation efficiency of up to 78 wt% at a feed weight ratio of 5.0%. The loading efficiency of the micelles was dependent on the length of the PCL arm in the A 2 B miktoarm polymers. Aqueous solubility of NIM was increased by w200 fold via micellar encapsulation. The in vitro release of NIM from the micelles was found to occur at a much slower rate than from its solution. Lipopolysaccharide induced nitric oxide production in N9 microglia cells was reduced in the presence of micelle-encapsulated NIM, as well as in the presence of micelles alone. The treatment of microglia with micelle-encapsulated NIM reduced the release of TNF-a, a pro-inflammatory cytokine. These results suggest that NIM-loaded miktoarm micelles could be useful in the treatment of neuroinflammation.

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“…Furthermore, Au NPs have recently attracted a lot of interest concerning biological applications [13]. Au NPs are readily incorporated by many different types of cells and were found to be suitable in nanomedicine [13] since they show low toxicity [14,15], have convenient characteristics, like the potential to be coated with target molecules [16], and can be used in biomedical imaging [3]. Different in vitro and in vivo studies made use of similar sized Au NPs, which were coated with medical substrates such as drugs or DNA [17][18][19].…”

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confidence: 99%

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

et al. 2014

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Aim:We examined cellular uptake mechanisms of fluorescently labeled polymer-coated gold nanoparticles (NPs) under different biological conditions by two quantitative, microscopic approaches. Materials & methods: Uptake mechanisms were evaluated using endocytotic inhibitors that were tested for specificity and cytotoxicity. Cellular uptake of gold NPs was analyzed either by laser scanning microscopy or transmission electron microscopy, and quantified by means of stereology using cells from the same experiment. Results: Optimal inhibitor conditions were only achieved with chlorpromazine (clathrin-mediated endocytosis) and methyl--cyclodextrin (caveolinmediated endocytosis). A significant methyl--cyclodextrin-mediated inhibition (63-69%) and chlorpromazine-mediated increase (43-98%) of intracellular NPs was demonstrated with both imaging techniques, suggesting a predominant uptake via caveolin-medicated endocytois. Transmission electron microscopy imaging revealed more than 95% of NPs localized in intracellular vesicles and approximately 150-times more NP events/cell were detected than by laser scanning microscopy. Conclusion: We emphasize the importance of studying NP-cell interactions under controlled experimental conditions and at adequate microscopic resolution in combination with stereology. Keywords:In recent years, various types of nanoparticles (NPs; <100 nm in all three dimensions, ISO TS 27687:2008) have been designed for potential biomedical and pharmaceutical applications, such as vehicles for targeted drug delivery, nucleic acids, biomedical imaging or biosensing [1][2][3]. Therefore, an increasing number of research studies have focused on the interaction of NPs with biocellular systems [4,5]. To analyze different types of NP-cell interactions, such as cellular NP uptake, it is essential to have valuable techniques for visualization and quantification, which are mainly based on spectroscopy or microscopy [6].The method of choice for the intracellular detection of NPs depends on the characteristics of the particles (fluorescence, size, and structure or electron density) and on the cellular structure of interest. Advantages of spectroscopy and different microscopic techniques have been reviewed in detail elsewhere [6,7]. In brief, spectroscopy such as inductively coupled plasma-mass spectroscopy [8] allows the quantification and detection of chemical NP elements within the cell, but does not provide any further information on the NP location within the cell. Fluorescence or laser scanning microscopy (LSM) allows real-time analysis or co-localization studies with fluorescently labeled structures [9]. Transmission electron microscopy (TEM) provides the highest resolution and allows quantification of NPs in sub cellular structures [10].

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One Example on How Colloidal Nano- and Microparticles Could Contribute to Medicine

Cited by 42 publications

References 94 publications

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

Tailoring the efficacy of nimodipine drug delivery using nanocarriers based on A2B miktoarm star polymers

Quantification of Gold Nanoparticle Cell Uptake Under Controlled Biological Conditions and Adequate Resolution

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