One Actor, Multiple Roles: The Performances of Cryptochrome in Drosophila

Damulewicz, Milena; Mazzotta, Gabriella

doi:10.3389/fphys.2020.00099

Cited by 24 publications

(22 citation statements)

References 172 publications

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“…Following the last reported, CRYs are the best candidate for biochemical magnetoreceptor that mediate light-dependent magnetosensitivity with radical-pair reaction, which associated with FAD¯° binding to CRY-photoreceptor proteins 16,19 . Furthermore, some recent experiments revealed that radical pair formation in the mammalian-type CRYs can occurs during magnetosensitivity by molecular oxygen and is not dependent to light [60][61][62] . CRYs can implicate to reinnervation and repairing of nervous systems in the mammalian brain as light-independent magnetosensor by an external low intensity EMF stimulations 63 .…”

Section: Discussionmentioning

confidence: 99%

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Human Cryptochrome Mediates Oxidative Stress Responses Modulated by Static and Electromagnetic Fields in Doxorubicin-Treated Cells

Hajipour-Verdom

Alipour

Abdolmaleki

et al. 2020

Preprint

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Cryptochromes (CRYs) have been suggested to involve in the magnetic sensing for navigation of migratory birds in response to Earth’s magnetic fields. Magnetic fields (MFs) through wireless penetration change the cell physiology by effect on physicochemical reactions. Here, we aimed to investigate the behavior of HEK 293T cells overexpressing human CRY-based magnetoreception complexes undergo doxorubicin (DOX) treatment and exposure to moderate intensity static magnetic field (SMF) and extremely low frequency-pulsed electromagnetic field (ELF-PEMF). The ability of this magnetosensor is depended on CRY-photoreceptor proteins that complemented with putative magnetosensor proteins (MagR). The results indicate that magnetic sensitivity of CRY-based magnetosensor can effects on the intracellular reactive oxygen species (ROS) production and cell growth, cell cycle progression and expression of DNA damage-related genes due to treatment of DOX, SMF and ELF-PEMF. Our findings show that ROS accumulation significantly decreased in the cells expressing CRY/MagR complexes in response to all treatments, and also cell viability is decreased in contrast to MFs exposure. In addition, magnetosensitive complexes mediated the upregulation of genes in the base excision repair (BER) pathway including ITPA in presence of SMF as well as MTH1 in presence of ELF-PEMF in the DOX treated-cells. Furthermore, CRY/MagR induced a remarkable cell cycle arrest at G0/G1 phase in the all treatments. These results demonstrated that CRY/MagR complexes modify the DNA damage responses during genotoxic stresses by controlling the cell cycle progression and ROS levels. Therefore, our data suggest that CRY-based magnetosensitive complexes can increase the cytotoxic effects of DOX even when SMF and/or ELF-PEMF exposure were provided, exclusively.

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Section: Discussionmentioning

confidence: 99%

“…In contrast, circadian system can protects tumor cells from chemotherapeutics. Therefore, CRYs play a pleiotropic role in the cells such related to p53 activity and cell cycle progression, and also DNA damage pathways, etc 62,68 .…”

Section: Discussionmentioning

confidence: 99%

Human Cryptochrome Mediates Oxidative Stress Responses Modulated by Static and Electromagnetic Fields in Doxorubicin-Treated Cells

Hajipour-Verdom

Alipour

Abdolmaleki

et al. 2020

Preprint

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“…The subsequent degradation of TIM leads to clock resetting (Koh et al., 2006; Lin et al., 2001; Naidoo, Song, Hunter‐Ensor, & Sehgal, 1999). Cry KO flies are resistant to light‐induced phase shifting, as well as free‐running in constant light conditions (Damulewicz & Mazzotta, 2020; Krishnan et al., 2001; Stanewsky et al., 1998; Tataroglu & Emery, 2015). Mutants of jet on the other hand, show no alterations in phenotypes in either light conditions or DD.…”

Section: Ubiquitination and Deubiquitination In The Clock Of Drosophilamentioning

confidence: 99%

To Ub or not to Ub: Regulation of circadian clocks by ubiquitination and deubiquitination

Srikanta

Cermakian

2020

Journal of Neurochemistry

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Circadian clocks are internal timing systems that enable organisms to adjust their behavioral and physiological rhythms to the daily changes of their environment. These clocks generate self‐sustained oscillations at the cellular, tissue, and behavioral level. The rhythm‐generating mechanism is based on a gene expression network with a delayed negative feedback loop that causes the transcripts to oscillate with a period of approximately 24 hr. This oscillatory nature of the proteins involved in this network necessitates that they are intrinsically unstable, with a short half‐life. Hence, post‐translational modifications (PTMs) are important to precisely time the presence, absence, and interactions of these proteins at appropriate times of the day. Ubiquitination and deubiquitination are counter‐balancing PTMs which play a key role in this regulatory process. In this review, we take a comprehensive look at the roles played by the processes of ubiquitination and deubiquitination in the clock machinery of the most commonly studied eukaryotic models of the circadian clock: plants, fungi, fruit flies, and mammals. We present the effects exerted by ubiquitinating and deubiquitinating enzymes on the stability, but also the activity, localization, and interactions of clock proteins. Overall, these PTMs have key roles in regulating not only the pace of the circadian clocks but also their response to external cues and their control of cellular functions.

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“…Cryptochrome (CRY) is a blue-light-sensitive protein ( VanVickle-Chavez and Van Gelder, 2007 ) playing many different roles, ranging from photoreceptor to magnetoreception and metabolism regulation (for review see Damulewicz and Mazzotta, 2020 ). It is expressed in a broad range of cells in the brain: in circadian pacemaker neurons (all five s-LNvs, l-LNvs, three of the six LNds, and some of the DN1s), but also in non-clock neurons, glia and visual system ( Benito et al, 2008 ; Yoshii et al, 2008 ; Damulewicz and Pyza, 2011 ; Fogle et al, 2011 ).…”

Section: How Do Light Inputs Modulate Sleep In Drosophila mentioning

confidence: 99%

Better Sleep at Night: How Light Influences Sleep in Drosophila

2020

Self Cite

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Sleep-like states have been described in Drosophila and the mechanisms and factors that generate and define sleep-wake profiles in this model organism are being thoroughly investigated. Sleep is controlled by both circadian and homeostatic mechanisms, and environmental factors such as light, temperature, and social stimuli are fundamental in shaping and confining sleep episodes into the correct time of the day. Among environmental cues, light seems to have a prominent function in modulating the timing of sleep during the 24 h and, in this review, we will discuss the role of light inputs in modulating the distribution of the fly sleep-wake cycles. This phenomenon is of growing interest in the modern society, where artificial light exposure during the night is a common trait, opening the possibility to study Drosophila as a model organism for investigating shift-work disorders.

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One Actor, Multiple Roles: The Performances of Cryptochrome in Drosophila

Cited by 24 publications

References 172 publications

Human Cryptochrome Mediates Oxidative Stress Responses Modulated by Static and Electromagnetic Fields in Doxorubicin-Treated Cells

Human Cryptochrome Mediates Oxidative Stress Responses Modulated by Static and Electromagnetic Fields in Doxorubicin-Treated Cells

To Ub or not to Ub: Regulation of circadian clocks by ubiquitination and deubiquitination

Better Sleep at Night: How Light Influences Sleep in Drosophila

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