One 3-oxoacyl-(acyl-Carrier-protein) reductase functions as 17β-hydroxysteroid dehydrogenase in the estrogen-degrading Pseudomonas putida SJTE-1

Wang, Pingping; Zheng, Daning; Wang, Yanqiu; Liang, Rubing

doi:10.1016/j.bbrc.2018.10.005

Cited by 16 publications

(12 citation statements)

References 21 publications

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“…There are some 17-HSDs that transform E2 to E1, homologous to the 17-HSD encoded by EGO55_02230 in N. tardaugens, i.e., OecA from Sphingomonas sp. KC8 encoded by KC8_09390 (20.09%) and the enzymes from Pseudomonas putida SJTE-1, 3-oxoacyl-ACP reductase, encoded by A210_09220 (34.06%) (Wang et al, 2018), and 17β-HSD, encoded by A210_19955 (37.90%) (Wang et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…The complete mineralization of estrogens to CO 2 can be accomplished by a limited number of bacteria, mainly from the phyla Proteobacteria and Actinobacteria (Fujii et al, 2002;Fahrbach et al, 2006;Yu et al, 2007Yu et al, , 2016Kurisu et al, 2010;Chen et al, 2017;Wang et al, 2019;Li et al, 2020). Although some biotransformation steps have been described in estrogen-degrading strains, the complete degradation pathway still remains unknown (Yu et al, 2013;Wang et al, 2014Wang et al, , 2018Wang et al, , 2019Chen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725

et al. 2020

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We have analyzed the catabolism of estrogens in Novosphingobium tardaugens NBRC 16725, which is able to use endocrine disruptors such as 17β-estradiol, estrone, and estriol as sole carbon and energy sources. A transcriptomic analysis enabled the identification of a cluster of catabolic genes (edc cluster) organized in two divergent operons that are involved in estrogen degradation. We have developed genetic tools for this estrogen-degrading bacterium, allowing us to delete by site-directed mutagenesis some of the genes of the edc cluster and complement them by using expression plasmids to better characterize their precise role in the estrogen catabolism. Based on these results, a catabolic pathway is proposed. The first enzyme of the pathway (17β-hydroxysteroid dehydrogenase) used to transform 17β-estradiol into estrone is encoded out of the cluster. A CYP450 encoded by the edcA gene performs the second metabolic step, i.e., the 4-hydroxylation of estrone in this strain. The edcB gene encodes a 4-hydroxyestrone-4,5-dioxygenase that opens ring A after 4-hydroxylation. The initial steps of the catabolism of estrogens and cholate proceed through different pathways. However, the degradation of estrogens converges with the degradation of testosterone in the final steps of the lower catabolic pathway used to degrade the common intermediate 3aα-H-4α(3′-propanoate)7a-β-methylhexahydro-1,5-indanedione (HIP). The TonB-dependent receptor protein EdcT appears to be involved in estrogen uptake, being the first time that this kind of proteins has been involved in steroid transport.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725

et al. 2020

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“…In the postmenopausal samples, some of the bacteria previously reported in the upper reproductive tract (Chen et al, 2017; Lee et al, 2019; Li et al, 2018) and involved in degradation of hormones (Wang et al, 2018b), e.g. Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

The vagino-cervical microbiome as a woman’s life history

Jie

Liang

et al. 2019

Preprint

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The vagina contains at least a billion microbial cells, which are dominated byLactobacilli. Here we perform metagenomic shotgun sequencing on cervical samples from 1148 women. Factors such as pregnancy, delivery histories and breast-feeding were all more important than menstrual cycle in shaping the microbiome. Bifidobacterium breve was seen with older age at sexual debut;Lactobacillus crispatus negatively correlated with pregnancy history; potential markers for lack of menstrual regularity, heavy flow, dysmenorrhea, contraceptives were also identified. Other features such as mood fluctuations and facial speckles could potentially be deduced from the vagino-cervical microbiome. Gut and oral microbiome, plasma vitamins, metals, amino acids and hormones showed associations with the vagino-cervical microbiome. Our results offer an unprecedented glimpse into the microbiota of the female reproductive tract and call for international collaborations to better understand its long-term health impact. Highlights:Shotgun sequencing of 1148 vagino-cervical samples reveal subtypes; 1Other omics such as moods and skin features could be predicted by the vagino-cervical 2 bacteria; 3Factors such as delivery mode and breast-feeding associate with the vagino-cervical 4 microbiome; 5With dwindled Lactobacilli, postmenopausal samples are relatively enriched for viruses. 6 7 3 4 vulvovaginal candidiasis (Bradford and Ravel, 2017). The over 90% of human 30 sequences in female reproductive tract samples, in contrast to 1% in feces (Li et al., 31 2018; Methé et al., 2012; Wang and Jia, 2016), has made metagenomic shotgun 32 sequencing more expensive. Most studies of the vaginal microbiota used 16S rRNA 33 gene amplicon sequencing, which lacked a view of the overall microorganism 34 communities including bacteria, archaea, viruses and fungi (Byrd et al., 2018), as well 35 as the functional capacity encoded. Besides infection, studies on current sexual activity 36 and the menstrual cycle occurred naturally to the vaginal microbiota field (Gajer et al., 37 2012; Ravel et al., 2010). However, lasting impacts from other potentially important 38 factors such as sexual debut, pregnancy and breast-feeding have not been looked at in 39 a reasonably large cohort. 40 41 As a sizable reservoir of microbes instead of a transient entity, the female reproductive 42 tract microbiota might also reflect conditions in other body sites. It is however not clear 43 whether other omics in circulation or in the intestine cross-talks with the vagino-cervical 44 microbiome. Intersecting with hormones, metabolic and immune functions, we find it 45intriguing to explore the potential link of the vagino-cervical microbiome to the brain and 46 the face. 47 5 data, immune indices, physical fitness test, facial skin imaging, as well as female life 53 history questionnaire, lifestyle questionnaire, and psychological questionnaire (Figure 1). 54Our work pinpoints other metadata or omics that can predict or be predicted from the 55 microbiota in the female reproductive tr...

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“…Increasing estrogen pollution in the environment has become one of the major challenges of the new age (Whitman 2017) and hence the quest for microbes with environmental estrogen-degrading abilities such as Gordonia sp. strain R9 (Liu et al 2020), Stenotrophomonas maltophilia ZL1 (Li et al 2012), Pseudomonas putida SJTE1 (Wang et al 2018). In this article, a new C. testosteroni strain JLU460ET was successfully obtained to degrade estrogens and testosterone from animal waste.…”

Section: Introductionmentioning

confidence: 99%

Identification and genome analysis of Comamonas testosteroni strain JLU460ET, a novel steroid-degrading bacterium

Liu

Shi

et al. 2021

3 Biotech

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In this work, C. testosteroni JLU460ET isolated from animal waste was confirmed to have great degradation capability for 17β-estradiol and testosterone. This bacterium could degrade nearly 90% of 17β-estradiol (5 mg L −1 ) in 4 days and transform it into estrone for further degradation. One hundred percent testosterone (144 mg L −1 ) could be completely degraded after 9 h of incubation. This is the first report of C. testosteroni strains with the ability to degrade both estrogens and testosterone. The whole genome sequence of C. testosteroni JLU460ET was obtained and annotated, containing one chromosome (5,497,097 bp) with 61.37% GC content. A total of 4805 protein-coding genes and 134 RNA genes (including 29 rRNA genes, 102 tRNA genes and three ncRNA genes) were identified. Furthermore, the complete genome sequence of C. testosteroni JLU460ET was compared with four other C. testosteroni strains. Altogether, these five C. testosteroni strains contain 3508 core genes and 7616 pan genes. A steroid degradation pathway including 11 steroid degradation genes exists in core genes of five C. testosteroni strains. Twenty-two steroid degradation genes were found in the C. testosteroni JLU460ET genome, which has the most reported steroid degradation genes among the five C. testosteroni genomes. Further functional genomic analysis identified a gene cluster responsible for testosterone degradation in C. testosteroni JLU460ET, as well as a gene encoding 17β-HSD, the key enzyme for transforming 17β-estradiol into estrone. This work could enrich the genome sources of steroid-degrading strains and promote the study of steroid-degradation mechanism in bacteria.

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One 3-oxoacyl-(acyl-Carrier-protein) reductase functions as 17β-hydroxysteroid dehydrogenase in the estrogen-degrading Pseudomonas putida SJTE-1

Cited by 16 publications

References 21 publications

Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725

Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725

The vagino-cervical microbiome as a woman’s life history

Identification and genome analysis of Comamonas testosteroni strain JLU460ET, a novel steroid-degrading bacterium

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