Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Cervical Cancer Cells Hela S3

Ni, Jiajun; Hualin, Feng; Xu, Xiang; T, Liu; Ye, Ting; Chen, Kan; Li, Gongchu

doi:10.3390/md19100532

Cited by 11 publications

(12 citation statements)

References 29 publications

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“…In contrast, Raf-1, with cross-talk between these pathways, impedes viral replication. Previous studies illustrated Sorafenib, which is an inhibitor targeting Raf kinases (Raf-1/C-Raf, wide-type B-Raf, and mutant B-Raf) [52], promoted oncoVV-AVL replication in Hela S3 cell lines [24]. Our current data illustrated that oncoVV-AVL replication was significantly downregulated in the presence of the inhibitors of PI3K/Akt, MAPK/ERK, and Hippo/MST pathways.…”

Section: Discussionsupporting

confidence: 66%

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Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells

et al. 2022

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Oncolytic vaccinia virus has been developed as a novel cancer therapeutic drug in recent years. Our previous studies demonstrated that the antitumor effect of oncolytic vaccina virus harboring Aphrocallistes vastus lectin (oncoVV-AVL) was significantly enhanced in several cancer cells. In the present study, we investigated the underlying mechanisms of AVL that affect virus replication and promote the antitumor efficacy of oncolytic virus in hepatocellular carcinoma (HCC). Our results showed that oncoVV-AVL markedly exhibited antitumor effects in both hepatocellular carcinoma cell lines and a xenograft mouse model. Further investigation illustrated that oncoVV-AVL could activate tumor immunity by upregulating the expression of type I interferons and enhance virus replication by inhibiting ISRE mediated viral defense response. In addition, we inferred that AVL promoted the ability of virus replication by regulating the PI3K/Akt, MAPK/ERK, and Hippo/MST pathways through cross-talk Raf-1, as well as metabolism-related pathways. These findings provide a novel perspective for the exploitation of marine lectins in oncolytic therapy.

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Section: Discussionsupporting

confidence: 66%

“…We have previously demonstrated that oncolytic vaccinia virus harboring Aphrocallistes vastus lectin (oncoVV-AVL) had substantial antitumor effects on colorectal cancer, hepatocellular carcinoma, and cervical cancer [23,24]. However, the mechanism of oncolysis remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells

et al. 2022

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“…Rodríguez et al reported that ERK activators increased viral yields, whereas ERK silencing decreased viral multiplication [ 38 ]. Our previous study found that oncoVV-AVL interfered with the Raf/ERK pathway in Hela S3 cells [ 22 ]. In Figure 5 a,d, although both Sorafenib (Raf inhibitor) and U0126 (MEK inhibitor) decreased oncoVV replication, the expression levels of both ERK and phosphorylated ERK (p-ERK) were not upregulated by oncoVV as compared with PBS, indicating that oncoVV replication in Hep-3B cells was independent of this pathway.…”

Section: Resultsmentioning

confidence: 99%

“…The oncolytic vaccinia virus (oncoVV) used in this research is a Western Reserve (WR) strain engineered for viral thymidine kinase (TK) gene deletion [ 19 ]. Our previous studies have shown that oncoVV armed with Tachypleus tridentatus lectin (oncoVV-TTL) [ 20 ], Aphrocallistes vastus lectin (oncoVV-AVL) [ 21 , 22 , 23 , 24 ], white-spotted charr lectin (oncoVV-WCL) [ 25 ], and Asterina pectinifera lectin (oncoVV-APL) elicited cytotoxicity to a variety of cancer cells by enhancing viral replication through the suppression of the antiviral response of cells and inducing cell apoptosis. In this study, the cytotoxic effects of four vaccinia virus variants constructed from marine lectins were compared, and the mechanisms of action of these viruses were further investigated in HCC.…”

Section: Introductionmentioning

confidence: 99%

Effects of Oncolytic Vaccinia Viruses Harboring Different Marine Lectins on Hepatocellular Carcinoma Cells

Zhou

Wang

Ying

et al. 2023

IJMS

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Oncolytic viruses are being developed as novel strategies for cancer therapy. Our previous studies have shown that vaccinia viruses armed with marine lectins improved the antitumor efficacy in diverse cancer types. The objective of this study was to assess the cytotoxic effects of oncoVV harboring Tachypleus tridentatus lectin (oncoVV-TTL), Aphrocallistes vastus lectin (oncoVV-AVL), white-spotted charr lectin (oncoVV-WCL), and Asterina pectinifera lectin (oncoVV-APL) on HCC. Our data revealed that the effects of recombinant viruses on Hep-3B cells were oncoVV-AVL > oncoVV-APL > oncoVV-TTL > oncoVV-WCL; oncoVV-AVL showed stronger cytotoxicity than oncoVV-APL, while oncoVV-TTL/WCL had no effect on cell killing in Huh7 cells, and PLC/PRF/5 cells exhibited sensitivity to oncoVV-AVL/TTL but not to oncoVV-APL/WCL. The cytotoxicity of oncoVV-lectins could be enhanced by apoptosis and replication in a cell-type-dependent manner. Further research revealed that AVL may mediate various pathways, including MAPK, Hippo, PI3K, lipid metabolism, and androgen pathways through AMPK crosstalk, to promote oncoVV replication in HCC in a cell-dependent manner. OncoVV-APL replication could be affected by AMPK/Hippo/lipid metabolism pathways in Hep-3B cells, AMPK/Hippo/PI3K/androgen pathways in Huh7 cells, and AMPK/Hippo pathways in PLC/PRF/5 cells. OncoVV-WCL replication was also multi-mechanistic, which could be affected by AMPK/JNK/lipid metabolism pathways in Hep-3B cells, AMPK/Hippo/androgen pathways in Huh7 cells, and AMPK/JNK/Hippo pathways in PLC/PRF/5 cells. In addition, AMPK and lipid metabolism pathways may play critical roles in oncoVV-TTL replication in Hep-3B cells, and oncoVV-TTL replication in Huh7 cells may depend on AMPK/PI3K/androgen pathways. This study provides evidence for the application of oncolytic vaccinia viruses in hepatocellular carcinoma.

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“…Genes encoding sponge ( Aphrocallistes vastus ) C-type lectin enhanced the cytotoxic action of oncolytic vaccinia virus (oncoVV) toward tumor cells. The complex formed between the lectin and the virus exerted an in vitro as well as an in vivo growth inhibitory action on Hela S3 cervical cancer cells [ 227 ].…”

Section: Other Biological Activities Of Lectinsmentioning

confidence: 99%

An Update of Lectins from Marine Organisms: Characterization, Extraction Methodology, and Potential Biofunctional Applications

et al. 2022

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Lectins are a unique group of nonimmune carbohydrate-binding proteins or glycoproteins that exhibit specific and reversible carbohydrate-binding activity in a non-catalytic manner. Lectins have diverse sources and are classified according to their origins, such as plant lectins, animal lectins, and fish lectins. Marine organisms including fish, crustaceans, and mollusks produce a myriad of lectins, including rhamnose binding lectins (RBL), fucose-binding lectins (FTL), mannose-binding lectin, galectins, galactose binding lectins, and C-type lectins. The widely used method of extracting lectins from marine samples is a simple two-step process employing a polar salt solution and purification by column chromatography. Lectins exert several immunomodulatory functions, including pathogen recognition, inflammatory reactions, participating in various hemocyte functions (e.g., agglutination), phagocytic reactions, among others. Lectins can also control cell proliferation, protein folding, RNA splicing, and trafficking of molecules. Due to their reported biological and pharmaceutical activities, lectins have attracted the attention of scientists and industries (i.e., food, biomedical, and pharmaceutical industries). Therefore, this review aims to update current information on lectins from marine organisms, their characterization, extraction, and biofunctionalities.

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Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Cervical Cancer Cells Hela S3

Cited by 11 publications

References 29 publications

Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells

Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells

Effects of Oncolytic Vaccinia Viruses Harboring Different Marine Lectins on Hepatocellular Carcinoma Cells

An Update of Lectins from Marine Organisms: Characterization, Extraction Methodology, and Potential Biofunctional Applications

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