Oncology stewardship in acute myeloid leukemia

Ochs, Madeleine A.; Marini, Bernard L.; Perissinotti, Anthony J.; Foucar, Charles; Pettit, Kristen; Burke, Patrick W.; Bixby, Dale; Benitez, Lydia

doi:10.1007/s00277-022-04872-1

Cited by 3 publications

(2 citation statements)

References 95 publications

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“…As the most common malignant disease of myeloid precursors in adult, AML with various genetic abnormalities has caused great difficulty for accurate risk stratification and treatment intensity [ 30 ]. Despite the progress of novel agents for the treatment paradigm, yet the optimal regimens for AML patients remain absent due to the limited guidance and the deficiency of pathogenesis [ 31 ]. For the purpose, in this study, we prepared resident and expanded AML-NKs and HD-NKs, and verified the alterations in the content of total NK cells and the subpopulations, together with the cellular vitality and cytotoxic activity.…”

Section: Discussionmentioning

confidence: 99%

Uncovering the cellular and omics characteristics of natural killer cells in the bone marrow microenvironment of patients with acute myeloid leukemia

Zhang,

Sun,

Xue

et al. 2024

Cancer Cell Int

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Background Acute myeloid leukemia (AML) is a highly heterogeneous hematologic malignancy and the most frequently acute leukemia of stem cell precursors and the myeloid derivatives in adult. Longitudinal studies have indicated the therapeutic landscape and drug resistance for patients with AML are still intractable, which largely attribute to the deficiency of detailed information upon the pathogenesis. Methods In this study, we compared the cellular phenotype of resident NK cells (rAML-NKs, rHD-NKs) and expanded NK cells (eAML-NKs, eHD-NKs) from bone marrow of AML patients (AML) and healthy donors (HD). Then, we took advantage of the co-culture strategy for the evaluation of the in vitro cytotoxicity of NK cells upon diverse tumor cell lines (e.g., K562, Nalm6, U937). With the aid of RNA-sequencing (RNA-SEQ) and bioinformatics analyses (e.g., GOBP analysis, KEGG analysis, GSEA, volcano plot), we verified the similarities and differences of the omics features between eAML-NKs and eHD-NKs. Results Herein, we verified the sharp decline in the content of total resident NK cells (CD3−CD56+) in rAML-NKs compared to rHD-NKs. Differ from the expanded eHD-NKs, eAML-NKs revealed decline in diverse NK cell subsets (NKG2D+, CD25+, NKp44+, NKp46+) and alterations in cellular vitality but conservations in cytotoxicity. According to transcriptomic analysis, AML-NKs and HD-NKs showed multifaceted distinctions in gene expression profiling and genetic variations. Conclusions Collectively, our data revealed the variations in the cytobiological and transcriptomic features between AML-NKs and HD-NKs in bone marrow environment. Our findings would benefit the further development of novel biomarkers for AML diagnosis and NK cell-based cytotherapy in future.

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Section: Discussionmentioning

confidence: 99%

Uncovering the cellular and omics characteristics of natural killer cells in the bone marrow microenvironment of patients with acute myeloid leukemia

Zhang,

Sun,

Xue

et al. 2024

Cancer Cell Int

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show abstract

“…al. 2022, defined oncology stewardship as a “set of coordinated strategies to improve the use of antineoplastic agents with the goal of enhancing patient outcomes while reducing financial toxicity.” 6 The key recommendation is the placement of pharmacists at the helm of the affairs. Aseptic compounding and clinical pharmacy services are necessary for oncology care settings and are being practiced in many nations, including LMICs.…”

mentioning

confidence: 99%