Oncocalyxone A Functions As an Anti-Glycation Agent In Vitro

Abstract

Advanced glycation endproducts (AGE) are the result of post-translational changes to proteins, which ultimately compromise their structure and/or function. The identification of methods to prevent the formation of these compounds holds great promise in the development of alternative therapies for diseases such as diabetes. Plants used in traditional medicine are often rich sources of anti-glycation agents. Therefore, in this study, we investigated the anti-glycation activity of one such compound, Oncocalyxone A (Onco A). Using spectrofluorimetric techniques, we determined that Onco A inhibits AGE formation in a concentration-dependent manner. Its IC50 value (87.88 ± 3.08 μM) was almost two times lower than the standard anti-glycation compound aminoguanidine (184.68 ± 4.85 μM). The excellent anti-glycation activity of Onco A makes it an exciting candidate for the treatment of diseases associated with excessive accumulation of AGE. However, additional studies are necessary to identify its mechanism of action, as well as the in vivo response in suitable model organisms.

Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil

“…( 2009 ) and Melo et al. ( 2015 ) with some modifications. Initially, the incubation medium consisting of BSA (1 mg/mL), glucose (10 mg/mL) and fructose (10 mg/mL) was prepared in sodium phosphate buffer (100 mmol/L pH 7.4).…”

Antidiabetic, Antiglycation, and Antioxidant Activities of Ethanolic Seed Extract of Passiflora edulis and Piceatannol In Vitro

“…The formation of AGEs was measured after incubation of a system containing protein and carbohydrates. This assay was based on previous methods [47]. The reaction system was obtained by adding 300 µL of plant extract or of the pure compound (at different concentrations), 150 µL D-fructose (200 mM), 150 µL of D-glucose (200 mM), and 300 µL of bovine serum albumin (BSA, 3 mg/mL) to a test tube.…”

(Poly)phenols of apples contribute to in vitro antidiabetic properties: Assessment of Canada's Apple Biodiversity Collection

“…The inhibitory assay of the AGE formation was performed as described by Melo et al (2015) with a minor modification of potassium phosphate buffer (PBS; 0.05 M) was used in place of sodium phosphate buffer (0.1 M). Apple (poly)phenol test extracts were prepared by evaporating the methanol and redissolving in 0.05 M PBS (pH 7.4; containing 100 mM NaCl, 0.02% NaN 3 [w/v]) before diluting to the appropriate concentrations (5-35 mg/ml).…”