On the two-step nature of bacteriophage adsorption

Stent, Gunther S.; Wollman, Elie L.

doi:10.1016/0006-3002(52)90041-3

Cited by 51 publications

(24 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…which is in agreement with previous measures of phage diffusion [62][63][64]. In the main text we stated that relevant processes for phages growing on bacteria are 452 given by the set of reactions (2).…”

supporting

confidence: 91%

See 1 more Smart Citation

CRISPR-based herd immunity limits phage epidemics in bacterial populations

Payne

Geyrhofer

Barton

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

supporting

confidence: 91%

“…Second, we examined the dynamics of the phage spread in different 60 environments -spatially structured and without structure. Furthermore, we developed 61 and analyzed a spatially explicit model of our experimental system to determine the 62 biologically relevant parameters necessary for bacterial populations to exhibit herd 63 immunity. 64…”

mentioning

confidence: 99%

CRISPR-based herd immunity limits phage epidemics in bacterial populations

Payne

Geyrhofer

Barton

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

“…The number of plaques was counted on each plate after overnight incubation at 37 "C, and the number of phages at each time was calculated. (For additional details, consult Adams, 1959;Brown, 1956;Eisenstark, 1967;Stent, 1963. ) Calculations.…”

Section: Methodsmentioning

confidence: 99%

Bacteriophage T4 Development Depends on the Physiology of its Host Escherichia Coli

et al. 1997

View full text Add to dashboard Cite

Several parameters of phage T4 adsorption to and growth in Escherichia coli B/r were determined. All changed monotonously with the bacterial growth rate (p), which was modified by nutritional conditions. Adsorption rate was faster at higher p values, positively correlated to cell size, and increased by pretreatment with low penicillin (Pn) concentrations; it was directly proportional to total cellular surface area, indicating a constant density of 14 receptors on cell envelopes irrespective of growth conditions. Parameters of phage development and cell lysis were pdependent. The rate of phage release and burst size increased, while the eclipse and latent periods decreased with increasing p. Differentiation between the contribution of several physiological parameters to the development of 14 was performed by manipulating the host cells. A competitive inhibitor of glucose uptake, methyl a-o-glucoside, was exploited to reduce the growth rate in the same effective carbon source. Synchronous cells were obtained by the 'baby-machine' and large cells were obtained by pretreatment with low Pn concentrations. Lysis was delayed by superinfection, and DNA content and concentration were modified by growing a thy mutant in limiting thymine concentrations. The results indicate that burst size is not limited by cell size or DNA composition, nor directly by the rate of metabolism, but rather by the rates of synthesis and assembly of phage components and by lysis time. The rates of synthesis and assembly of phage components seem to depend on the content of the protein-synthesizing system and lysis time seems to depend on cellular dimensions.

show abstract

“…The most widely accepted is a two-step adsorption process consisting of a reversible and irreversible reaction, proposed by Stent and Wollman [22], which has the advantage of not only being in close agreement with the physical phenomenon, but also providing an adequate fit for multiple phage/host systems [22][23][24]. However, in the case of activated bacteriophage T4, the virus particles have a very low probability of becoming detached once their tail fibers come in contact with the cell surface [25]; hence the reaction scheme can be approximated by the model shown in Eqs.…”

Section: Model Developmentmentioning

confidence: 99%

Bacteriophage adsorption efficiency and its effect on amplification

Storms

Arsenault

Sauvageau

et al. 2010

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Existing models for bacteriophage adsorption are modified with the addition of a new term, adsorption efficiency, and applied to a T4-Escherichia coli system. The adsorption efficiency is the fraction of phage that adsorbs irreversibly to the host. Adsorption kinetics were modeled using the adsorption rate constant(k) and the adsorption efficiency(epsilon). Experimental data demonstrated that the adsorption rate constant depends strongly on the condition of the host while the adsorption efficiency is a property of the bacteriophage population. The adsorption efficiency exhibited a marked dependence on the concentration of L-tryptophan. The system was used to study the effect of adsorption kinetics on bacteriophage amplification. Increasing adsorption efficiency had an effect similar to increasing the initial multiplicity of infection; the number of phages produced during amplification decreased. Optimizing the adsorption efficiency by manipulating the L-tryptophan concentration yielded a 14-fold increase in the number of phages produced.

show abstract

On the two-step nature of bacteriophage adsorption

Cited by 51 publications

References 7 publications

CRISPR-based herd immunity limits phage epidemics in bacterial populations

CRISPR-based herd immunity limits phage epidemics in bacterial populations

Bacteriophage T4 Development Depends on the Physiology of its Host Escherichia Coli

Bacteriophage adsorption efficiency and its effect on amplification

Contact Info

Product

Resources

About