ON THE STERILIZATION OF <i>DROSOPHILA</i> BY HIGH TEMPERATURE

Young, William C.; Plough, Harold Henry

doi:10.2307/1536478

Cited by 42 publications

(22 citation statements)

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“…At 32°C the number of eggs laid per day by D. melanogaster was found by Clavel and Clavel(1969) to be less than half the maximum daily production (which occurred at about 28°C) and negligible compared to the total life-time fecundity maximum (occurring at 24OC). Young and Plough (1926) found that at 32°C a proportion of D. melanogaster males were rendered permanently sterile after 10-13 days. Exposure to 33.5"C for 24 h has been shown to significantly reduce longevity of 7-day-old adult D. melanogaster (Hosgood and Parsons 1968).…”

Section: Discussionmentioning

confidence: 97%

Adaptive behaviour of Drosophila adults in relation to temperature and humidity

Prince¹,

Pa²

1977

Aust. J. Zool.

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The reactions of three Drosophila species (D. melanogaster, D. simulans and D. immigrans) were studied in a temperature gradient at different humidities. In a saturation-deficit gradient all three species showed adaptive behaviour which could be related to their physiological resistance to desiccation. This is taken as experimental evidence for similar avoidance of stressful conditions in the field.

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Section: Discussionmentioning

confidence: 97%

Adaptive behaviour of Drosophila adults in relation to temperature and humidity

Prince¹,

Pa²

1977

Aust. J. Zool.

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“…It has long been known that primary spermatocytes are sensitive to both cold and hot stresses (Young and Plough, 1926;Sanders and Ayles, 1970; David et al, 1971; Cohet, 1973; Ayles et al, 1973;Suchowersky et al, 1974). Since the most typical feature of these cells is the presence of lampbrush-like loops, we checked whether heat stress can affect these intranuclear structures.…”

Section: Journal Of Cell Science 1610mentioning

confidence: 99%

“…Under these conditions, male flies become sterile (Young and Plough, 1926) and data from the literature demonstrate that sterility is indeed generated in primary spermatocytes (Ayles et al, 1973). Testes prepared from these males and analyzed by phase-contrast microscopy show apparently normal Y-loops.…”

Section: Triplexes Are a Conserved Feature Of Y-loops In Drosophilidsmentioning

confidence: 99%

“…We hypothesize that these triplexes are able to mediate the interaction between DNAspecific regions and some proteins that might recognize triplex structure as a specific binding site. Indeed, at least one triplexbinding protein has been already identified in D. melanogaster (Jimenez-Garcia et al, 1998).It has long been known that primary spermatocytes are sensitive to both cold and hot stresses (Young and Plough, 1926;Sanders and Ayles, 1970; David et al, 1971; Cohet, 1973; Ayles et al, 1973;Suchowersky et al, 1974). Since the most typical feature of these cells is the presence of lampbrush-like loops, we checked whether heat stress can affect these intranuclear structures.…”

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confidence: 99%

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Drosophila melanogaster kl-3 and kl-5 Y-loops harbor triple-stranded nucleic acids

Piergentili

Mencarelli

2008

Journal of Cell Science

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Primary spermatocyte nuclei of Drosophila melanogaster contain three prominent lampbrush-like loops. The development of these structures has been associated with the transcription of three fertility factors located on the Y chromosome, named kl-5, kl-3 and ks-1. These loci have huge physical dimensions and contain extremely long introns. In addition, kl-3 and kl-5 were shown to encode two putative dynein subunits required for the correct assembly of the sperm axoneme. Here, we show that both the kl-5 and kl-3 loops are intensely decorated by monoclonal antibodies recognizing triple-stranded nucleic acids, and that each loop presents a peculiar molecular organization of triplex structures. Moreover, immunostaining of Drosophila hydei primary spermatocytes revealed that also in this species -which diverged from D. melanogaster 58 million years ago -Y-loops are decorated by anti-triplex antibodies, strongly suggesting a conserved role of loop-associated triplexes. Finally, we showed that in D. melanogaster wild-type lines that are raised at the non-permissive temperature of 31±0.5°C (which is known to induce male sterility in flies) both the triplex immunostaining and the axonemal dynein heavy chains encoded by kl-3 and kl-5 are no longer detectable, which suggests a functional correlation between loop-associated triplexes, the presence of axonemal proteins and male fertility in fly. Journal of Cell Science 1606 triplexes, the most stable triads are those involving a protonated cytosine (C + ) formed by C + *GC triad and T*AT nucleotides (the asterisks mark the nucleotides on the third strand). Notably, these sequences are overrepresented in all eukaryotic genomes (Behe, 1987; Behe, 1995), as well as in eukaryotic viruses (Beasty and Behe, 1988). It is also possible to have triplex formation without homopurine stretches, and these triplexes seem to be as stable as the others at least under certain conditions (Dayn et al., 1992). Although triplexes are well characterized in vitro, their biological significance in living organisms is still under discussion (Zain and Sun, 2003). It has been demonstrated that, upon formation, these structures can frequently downregulate (Cooney et al., 1988; Birg et al., 1990; Faria et al., 2000;Faria et al., 2001) and sometimes upregulate (reviewed in Faria and Giovannangeli, 2001) gene expression, a fact which demonstrates their potential in gene control and suggests their use in gene therapy (Wang et al., 1995;Vasquez et al., 2000) (for a review, see Rogers et al., 2005). The structures are also able to impair DNA polymerization (Dayn et al., 1992), and can influence DNA recombination and repair (Faruqi et al., 1996;Wang et al., 1996;Faruqi et al., 2000;Vasquez et al., 2002;Kalish et al., 2005;Raghavan et al., 2005) (for reviews, see Seidman and Glazer, 2003; Chin et al., 2007). Triplexes might also have a role in chromatin organization of both interphase nuclei (Agazie et al., 1996;Ohno et al., 2002) and mitotic chromosomes . Recently, it has been demonstrated that a triplestran...

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“…Particularly with D. melanogaster, the influence of developmental temperature has been considered for a number of characters. These include developmental rate (Davidson 1944), the probability of development from egg to adult and the associated sex ratio (Tantawy and Mallah 1961), longevity (Cohet 1973), fecundity (David and Clavel 1969) and fertility (Young and Plough 1926). Some measures are also available for D. simulans but this species has not been as well studied (Parsons 1975).…”

Section: Introductionmentioning

confidence: 99%

The effect of developmental temperature on population flexibility in Drosophila melanogaster and D. simulans

McKenzie¹

1978

Aust. J. Zool.

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Ten strains each of D. melanogaster and D, simulans were derived from single inseminated females of the same population. For each strain the influence of developmental temperatures in the range 12-30°C was considered for developmental time, egg to adult development percentage, sex ratio, longevity of adults, mating speed, fecundity and fertility. The species showed similar responses for all characters, although for the latter four D. simulans was more affected by extreme temperatures. Analyses of variance demonstrated temperature, strain, and temperaturex strain effects to be generally significant. For sex ratio, however, temperature effects alone were significant. The results are discussed in relation to the level of genetical integration existing in a population and how such integration allows for considerable population flexibility.

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ON THE STERILIZATION OF DROSOPHILA BY HIGH TEMPERATURE

Cited by 42 publications

References 0 publications

Adaptive behaviour of Drosophila adults in relation to temperature and humidity

Adaptive behaviour of Drosophila adults in relation to temperature and humidity

Drosophila melanogaster kl-3 and kl-5 Y-loops harbor triple-stranded nucleic acids

The effect of developmental temperature on population flexibility in Drosophila melanogaster and D. simulans

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