On the Spelling of 'Clon'

Giddings, Franklin H.

doi:10.1126/science.22.555.206-a

Cited by 12 publications

(3 citation statements)

References 1 publication

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“…The electrical feature of surface charge requires that the charge‐based particle separation involves the use of electric field. Such a separation has been demonstrated in CE and electrical field flow fractionation , both of which take place in a batch process along the axis of time. Charge‐based separation of particles has also been realized in a continuous flow along the axis of space.…”

Section: Introductionmentioning

confidence: 99%

Charge‐based separation of particles and cells with similar sizes via the wall‐induced electrical lift

Thomas

Todd

et al. 2016

Electrophoresis

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The separation of particles and cells in a uniform mixture has been extensively studied as a necessity in many chemical and biomedical engineering and research fields. This work demonstrates a continuous charge-based separation of fluorescent and plain spherical polystyrene particles with comparable sizes in a ψ-shaped microchannel via the wall-induced electrical lift. The effects of both the direct current electric field in the main-branch and the electric field ratio in between the inlet branches for sheath fluid and particle mixture are investigated on this electrokinetic particle separation. A Lagrangian tracking method based theoretical model is also developed to understand the particle transport in the microchannel and simulate the parametric effects on particle separation. Moreover, the demonstrated charge-based separation is applied to a mixture of yeast cells and polystyrene particles with similar sizes. Good separation efficiency and purity are achieved for both the cells and the particles.

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Section: Introductionmentioning

confidence: 99%

Charge‐based separation of particles and cells with similar sizes via the wall‐induced electrical lift

Thomas

Todd

et al. 2016

Electrophoresis

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“…Charge‐based particle separation has been achieved in microfluidic devices using several approaches, which can be classified as batch‐wise or continuous flow based on the separation process. The former includes capillary‐based electrophoresis and electrical field‐flow fractionation , where particles of dissimilar charges migrate through a separation column at different times due to their unequal electrophoretic velocities and their residences in stream laminas of unequal velocities , respectively. Free‐flow electrophoresis is a continuous‐flow approach , where particles of dissimilar charges are split up into different lanes by their transverse electrophoretic migrations relative to a pressure‐driven carrier electrolyte flow .…”

Section: Introductionmentioning

confidence: 99%

Reservoir‐based dielectrophoresis for microfluidic particle separation by charge

2013

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The separation of particles from a complex mixture is important to a wide range of applications in industry, biology, medicine etc. This work demonstrates a microfluidic approach to separate similar-sized fluorescent and nonfluorescent particles based upon the difference in their surface charges inside a reservoir. Such a separation exploits the reservoir-based dielectrophoresis, which is induced by the inherent electric field gradient formed at the reservoir-microchannel junction, to isolate the trapped fluorescent particles within the reservoir from the streaming nonfluorescent particles. The effects of the DC field magnitude (or equivalently the electrokinetic flow magnitude) and the AC field frequency of DC-biased AC electric fields are investigated on particle separation. A numerical model is also developed to simulate the electrokinetic transport behaviors of the two types of particles. This demonstrated reservoir-based dielectrophoresis particle sorter can operate in parallel to increase the flow throughput. It is suitable for integration with other functional parts into lab-on-a-chip devices for diverse particle handling.

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“…size, charge, and density) is simpler, which can take place in either a batchwise or a continuous‐flow process 3–5. The batchwise process separation includes filtration 12, chromatography 13, electrophoresis 14, and field‐flow fractionation 15, where only a particular volume of sample undergoes the separation and the components are separated in the time axis. In the continuous‐flow separation, a focused stream of particle mixture is introduced to the separation channel continuously.…”

Section: Introductionmentioning

confidence: 99%

Continuous dielectrophoretic separation of particles in a spiral microchannel

2010

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Particle separation is a fundamental operation in the areas of biology and physical chemistry. A variety of force fields have been used to separate particles in microfluidic devices, among which electric field may be the most popular one due to its general applicability and adaptability. So far, however, electrophoresis-based separations have been limited primarily to batchwise processes. Dielectrophoresis (DEP)-based separations require in-channel micro-electrodes or micro-insulators to produce electric field gradients. This article introduces a novel particle separation technique in DC electrokinetic flow through a planar double-spiral microchannel. The continuous separation arises from the cross-stream dielectrophoretic motion of particles induced by the non-uniform electric field inherent to curved channels. Specifically, particles are focused by DEP to one sidewall of the first spiral, and then dielectrophoretically deflected toward the other sidewall of the second spiral at a particle-dependent rate, leading to focused particle streams along different flow paths. This DEP-based particle separation technique is demonstrated in an asymmetric double-spiral microchannel by continuously separating a mixture of 5/10 microm particles and 3/5 microm particles.

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On the Spelling of 'Clon'

Cited by 12 publications

References 1 publication

Charge‐based separation of particles and cells with similar sizes via the wall‐induced electrical lift

Charge‐based separation of particles and cells with similar sizes via the wall‐induced electrical lift

Reservoir‐based dielectrophoresis for microfluidic particle separation by charge

Continuous dielectrophoretic separation of particles in a spiral microchannel

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