Charge‐based separation of particles and cells with similar sizes via the wall‐induced electrical lift

Thomas, Cory; Lu, Xinglin; Todd, Andrew; Raval, Yash S.; Tzeng, Tzuen‐Rong; Song, Yongxin; Wang, Junsheng; Li, Dongqing; Xuan, Xiangchun

doi:10.1002/elps.201600284

Cited by 12 publications

(10 citation statements)

References 48 publications

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“…Thomas et al. demonstrated a sheath‐flow separation of yeast cells from 5 µm polystyrene particles by charge in the electroosmotic flow of a density‐matched phosphate buffer/glycerol solution through a ψ‐shaped microchannel (Fig. , upper panel) via the wall‐induced electrical lift.…”

Section: Straight Microchannels With Uniform Cross Sectionsmentioning

confidence: 99%

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

Xuan

2019

Electrophoresis

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Microfluidic devices have been extensively used to achieve precise transport and placement of a variety of particles for numerous applications. A range of force fields have thus far been demonstrated to control the motion of particles in microchannels. Among them, electric field‐driven particle manipulation may be the most popular and versatile technique because of its general applicability and adaptability as well as the ease of operation and integration into lab‐on‐a‐chip systems. This article is aimed to review the recent advances in direct current (DC) (and as well DC‐biased alternating current) electrokinetic manipulation of particles for microfluidic applications. The electric voltages are applied through electrodes that are positioned into the distant channel‐end reservoirs for a concurrent transport of the suspending fluid and manipulation of the suspended particles. The focus of this review is upon the cross‐stream nonlinear electrokinetic motions of particles in the linear electroosmotic flow of fluids, which enable the diverse control of particle transport in microchannels via the wall‐induced electrical lift and/or the insulating structure‐induced dielectrophoretic force.

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Section: Straight Microchannels With Uniform Cross Sectionsmentioning

confidence: 99%

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

Xuan

2019

Electrophoresis

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“…The devices can incorporate various processing techniques such as acoustophoresis [7,8], magnetophoresis [9,10], dielectrophoresis (DEP) [11,12], and hydrophoresis [13]. DEP is frequently used to capture or separate particles [14,15] and biological cells such as tumor cells [16,17], yeast cells [18,19], and microorganisms [20,21]. For the diagnosis of malaria, the case of low parasitemia is challenging.…”

Section: Introductionmentioning

confidence: 99%

A discrete dielectrophoresis device for the separation of malaria‐infected cells

et al. 2022

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Malaria is a serious disease caused by Plasmodium parasites that infect red blood cells (RBCs). This paper presents the continuous separation of malaria‐infected RBCs (iRBCs) from normal blood cells. The proposed method employed the discrete dielectrophoresis (DEP) in a microfluidic device with interdigitated electrodes. Our aim is to treat a sample having high concentration of cells to realize high throughput and to prevent the clogging of the microchannel with the use of the discrete DEP. The discrete DEP force for deflecting cells in the device was controlled by adjusting the magnitude, frequency, and duty cycle of the applied voltage. The effectiveness of the proposed method was demonstrated by separating the malaria‐infected cells in samples having a cell concentration of 106 cells/µl. From experimental results, we determined the enrichment that is needed to enhance the detection in the case of low parasitemia. The enrichment of the infected cells at the device output was 3000 times as high as that of the input containing 1 infected cell to 106 normal cells. Therefore, the proposed method is highly effective and can significantly facilitate the detection of the infected cells for the identification of Malaria patients.

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“…It has also been used to separate particles of different diameters due to its strong dependence on particle size . Moreover, the electrical lift has been demonstrated to separate particles based on the difference in surface charge , or equivalently, the particle zeta potential that has a direct impact on the electrokinetic motion .…”

Section: Introductionmentioning

confidence: 99%

Revisit of wall‐induced lateral migration in particle electrophoresis through a straight rectangular microchannel: Effects of particle zeta potential

Liu

Saffarian

et al. 2018

Electrophoresis

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Previous studies have reported a lateral migration in particle electrophoresis through a straight rectangular microchannel. This phenomenon arises from the inherent wall-induced electrical lift that can be exploited to focus and separate particles for microfluidic applications. Such a dielectrophoretic-like force has been recently found to vary with the buffer concentration. We demonstrate in this work that the particle zeta potential also has a significant effect on the wall-induced electrical lift. We perform an experimental study of the lateral migration of equal-sized polystyrene particles with varying surface charges under identical electrokinetic flow conditions. Surprisingly, an enhanced focusing is observed for particles with a faster electrokinetic motion, which indicates a substantially larger electrical lift for particles with a smaller zeta potential. We speculate this phenomenon may be correlated with the particle surface conduction that is a strong function of particle and fluid properties.

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Charge‐based separation of particles and cells with similar sizes via the wall‐induced electrical lift

Cited by 12 publications

References 48 publications

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

Recent advances in direct current electrokinetic manipulation of particles for microfluidic applications

A discrete dielectrophoresis device for the separation of malaria‐infected cells

Revisit of wall‐induced lateral migration in particle electrophoresis through a straight rectangular microchannel: Effects of particle zeta potential

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