On the Radiomimetic Effects of Cupferron and Potassium Cyanide

Kihlman, B.A.

doi:10.1083/jcb.5.2.351

Cited by 11 publications

(2 citation statements)

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“…We have devised a modification of the conventional protoplast fusion procedure in which cells of one of the haploid strains (the donor strain) have been killed by treatment with the chromosome-fragmenting agents cupferron or hydroxylamine. The chromosome-breaking properties of these two chemicals have been well established in other systems [8,10,11]. It has been demonstrated (unpublished results) using contour clamped homogeneous-electric field electrophoresis (CHEF) that treatment of S. cerevisiae with these agents destroys the normal chromosome banding pat-tern, thus indicating that chromosome fragmentation is occurring here also.…”

Section: Discussionmentioning

confidence: 88%

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Multiple transformation of Saccharomyces cerevisiae by protoplast fusion

Reeves

1992

FEMS Microbiology Letters

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A technique for the multiple transformation of yeast by protoplast fusion is described. This involved the PEG-induced fusion of protoplasts from cells which had been treated with chromosome-fragmenting agents (in this case cupferron and hydroxylamine) with protoplasts of triply auxotrophic cells. The recovery of transformants was increased significantly if one of the amino acid requirements of the recipient strain was included in the selection medium. Transformants isolated on supplemented media remained auxotrophic for that requirement. Prototrophic, uninucleate transformants had a DNA content and cellular volume similar to that of the parental strains. Possible mechanisms of gene transfer are discussed. This technique offers the possibility of transferring desirable characteristics from one yeast strain to another without altering the ploidy level of the recipient strain.

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Section: Discussionmentioning

confidence: 88%

“…Cupferron acts in a similar manner and also functions as a chelating agent capable of forming complexes with iron and copper. The chromosome-fragmenting effects of cupferron were discovered using root cells of Vicia faba [10], inducing damage to the chromatids [11].…”

Section: Introductionmentioning

confidence: 99%