Multiple transformation of Saccharomyces cerevisiae by protoplast fusion

Reeves, Emer P.

doi:10.1016/0378-1097(92)90024-i

Cited by 2 publications

(1 citation statement)

References 12 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research from the 1970s found that HA reacts with the formyl group in 5-formyltetrahyrofolate, and likely other folates, in S. cerevisiae ( Reeves, Kavanagh, and Whittaker 1992 ). This reaction forms formoxime, which was shown as an inhibitor of thymidylate synthetase in E. coli ( Klein et al .…”

Section: Resultsmentioning

confidence: 99%

Profiling the compendium of changes in Saccharomyces cerevisiae due to mutations that alter availability of the main methyl donor S-Adenosylmethionine

Remines,

Schoonover,

Knox

et al. 2024

G3: Genes, Genomes, Genetics

View full text Add to dashboard Cite

The SAM1 and SAM2 genes encode for S-AdenosylMethionine (AdoMet) synthetase enzymes, with AdoMet serving as the main cellular methyl donor. We have previously shown that independent deletion of these genes alters chromosome stability and AdoMet concentrations in opposite ways in Saccharomyces cerevisiae. To characterize other changes occurring in these mutants, we grew wildtype, sam1Δ/sam1Δ, and sam2Δ/sam2Δ strains in 15 different Phenotypic Microarray plates with different components and measured growth variations. RNA-Sequencing was also carried out on these strains and differential gene expression determined for each mutant. We explored how the phenotypic growth differences are linked to the altered gene expression, and hypothesize mechanisms by which loss of the SAM genes and subsequent AdoMet level changes, impact pathways and processes. We present six stories, discussing changes in sensitivity or resistance to azoles, cisplatin, oxidative stress, arginine biosynthesis perturbations, DNA synthesis inhibitors, and tamoxifen, to demonstrate the power of this novel methodology to broadly profile changes due to gene mutations. The large number of conditions that result in altered growth, as well as the large number of differentially expressed genes with wide-ranging functionality, speaks to the broad array of impacts that altering methyl donor abundance can impart. Our findings demonstrate that some cellular changes are directly related to AdoMet-dependent methyltransferases and AdoMet availability, some are directly linked to the methyl cycle and its role in production of several important cellular components, and others reveal impacts of SAM gene mutations on previously unconnected pathways.

show abstract

Section: Resultsmentioning

confidence: 99%

Profiling the compendium of changes in Saccharomyces cerevisiae due to mutations that alter availability of the main methyl donor S-Adenosylmethionine

Remines,

Schoonover,

Knox

et al. 2024

G3: Genes, Genomes, Genetics

View full text Add to dashboard Cite

show abstract

Profiling The Compendium Of Changes InSaccharomyces cerevisiaeDue To Mutations That Alter Availability Of The Main Methyl Donor S-Adenosylmethionine

Remines,

Schoonover,

Knox

et al. 2023

Preprint

View full text Add to dashboard Cite

TheSAM1andSAM2genes encode for S-AdenosylMethionine (AdoMet) synthetase enzymes, with AdoMet serving as the main methyl donor. We have previously shown that independent deletion of these genes alters chromosome stability and AdoMet concentrations in opposite ways inS. cerevisiae. To characterize other changes occurring in these mutants, we grew wildtype,sam1Δ/sam11Δ,andsam21Δ/sam21Δstrains in 15 different Phenotypic Microarray plates with different components, equal to 1440 wells, and measured for growth variations. RNA-Sequencing was also carried out on these strains and differential gene expression determined for each mutant. In this study, we explore how the phenotypic growth differences are linked to the altered gene expression, and thereby predict the mechanisms by which loss of theSAMgenes and subsequent AdoMet level changes, impactS. cerevisiaepathways and processes. We present six stories, discussing changes in sensitivity or resistance to azoles, cisplatin, oxidative stress, arginine biosynthesis perturbations, DNA synthesis inhibitors, and tamoxifen, to demonstrate the power of this novel methodology to broadly profile changes due to gene mutations. The large number of conditions that result in altered growth, as well as the large number of differentially expressed genes with wide-ranging functionality, speaks to the broad array of impacts that altering methyl donor abundance can impart, even when the conditions tested were not specifically selected as targeting known methyl involving pathways. Our findings demonstrate that some cellular changes are directly related to AdoMet-dependent methyltransferases and AdoMet availability, some are directly linked to the methyl cycle and its role is production of several important cellular components, and others reveal impacts of SAM gene mutations on previously unconnected pathways.

show abstract

Multiple transformation of Saccharomyces cerevisiae by protoplast fusion

Cited by 2 publications

References 12 publications

Profiling the compendium of changes in Saccharomyces cerevisiae due to mutations that alter availability of the main methyl donor S-Adenosylmethionine

Profiling the compendium of changes in Saccharomyces cerevisiae due to mutations that alter availability of the main methyl donor S-Adenosylmethionine

Profiling The Compendium Of Changes InSaccharomyces cerevisiaeDue To Mutations That Alter Availability Of The Main Methyl Donor S-Adenosylmethionine

Contact Info

Product

Resources

About