On the problem of lamination in the superficial dorsal horn of mammals: A reappraisal of the substantia gelatinosa in postnatal life

Woodbury, C. Jeffery; Ritter, Amy M.; Koerber, H. Richard

doi:10.1002/(sici)1096-9861(20000131)417:1<88::aid-cne7>3.0.co;2-u

Cited by 81 publications

(55 citation statements)

References 81 publications

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“…One possibility is that this more superficial termination of D-HFAs is more apparent than real, because the SG appears to be thicker in mice than in larger species (see discussion in Woodbury et al, 2000); thus, whereas a ventral expansion of this region may explain a relatively more dorsal termination of mouse D-HFAs overall, it cannot explain D-hair arbors abutting the lamina I/II border (see Results). A related possibility is that this more superficial termination reflects significant species and/or segmental differences in D-HFAs themselves, as previous studies in adults were conducted at caudal levels in cat and monkey or cranial levels in cat (Hayashi, 1985).…”

Section: Substantia Gelatinosa: Pleasure or Pain?mentioning

confidence: 95%

“…The latter preferentially binds myelinated cutaneous afferents in rodents and has been widely used in studies of nerve central labeling patterns in normal animals (for review, see Woodbury et al, 2001). Because labeling is observed in lamina I after cholera toxin transport (Woodbury et al, 2000), it is unlikely that myelinated nociceptors simply fail to transport this marker. A more likely explanation is that bulk-transport techniques simply lack sufficient sensitivity for visualization of these diffuse inputs.…”

Section: Lamina I-v Nociceptorsmentioning

confidence: 99%

“…However, observations of isolated D-hair arbors invading "the deep two-thirds of lamina II" in the spinal trigeminal nucleus caudalis of cat (Hayashi, 1985) not only suggest otherwise, but may help explain the substantial portion of cat SG that remains unlabeled at spinal levels after isolectin-B 4 transport (Woodbury et al, 2000). Moreover, a more superficial termination of tactile afferents than commonly held is supported by the finding that type II synaptic glomeruli, formed by D-HFAs (Rethelyi et al, 1982), constitute ϳ20% of the glomerular structures in lamina IIo (Ribeiro-da-Silva and Coimbra, 1982).…”

Section: Substantia Gelatinosa: Pleasure or Pain?mentioning

confidence: 99%

“…If true, this would predict that the growth of myelinated nociceptor central projections is initially exuberant and these arbors subsequently undergo extensive pruning during early postnatal life or, alternatively, that the cells responsible for these widespread SG projections represent a transient population that is lost through cell death postnatally. However, uncertainty surrounds the identities of afferents that transport cholera toxin (Tong et al, 1999;Woodbury et al, 2001;Sántha and Jancsó , 2003), and bulklabeling studies may lack sufficient power to resolve this important question regarding SG functional organization (Woodbury et al, 2000(Woodbury et al, , 2001Woodbury and Koerber, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Myelinated Skin Sensory Neurons Project Extensively throughout Adult Mouse Substantia Gelatinosa

Boada¹,

Woodbury²

2008

J. Neurosci.

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The substantia gelatinosa (SG) of the dorsal horn of the spinal cord is a recipient zone for unmyelinated sensory neurons in adults. Recent studies of the central anatomy of physiologically identified skin sensory neurons in neonatal mice have shown that this region also receives substantial inputs from a variety of myelinated afferents. The present experiments were performed to determine whether these neonatal inputs represent a transient phenotype that retracts from the SG. Studies were conducted in an in vivo spinal cord preparation from adult mice; thoracic levels were targeted to facilitate comparisons with previous in vitro findings. We show that the SG continues to receive substantial projections from myelinated skin sensory neurons throughout life. A large population of myelinated nociceptors conducting in the upper A␦ and low A␤ range maintained extensive projections throughout all areas of the SG well into adulthood; the latter gave rise to dorsally recurving "flame"-shaped arbors extending into the marginal layer that were identical to afferents described in neonates and after nerve injury in adult rats. Furthermore, exquisitely sensitive down hair follicle afferents projected throughout the inner half of the SG (i.e., lamina IIi) and sent dense clusters of terminals well into the outer SG (IIo), where they intermingled with those of unmyelinated nociceptors. Arguments are presented that the SG likely plays a predominant role in tactile processing under normal conditions, but that this role switches rapidly to nociceptive-only during environmental exigencies imposed by temperature extremes.

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Section: Substantia Gelatinosa: Pleasure or Pain?mentioning

confidence: 95%

Section: Lamina I-v Nociceptorsmentioning

confidence: 99%

Section: Substantia Gelatinosa: Pleasure or Pain?mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Myelinated Skin Sensory Neurons Project Extensively throughout Adult Mouse Substantia Gelatinosa

Boada¹,

Woodbury²

2008

J. Neurosci.

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“…Lamina II can be subdivided into inner (IIi) and outer (IIo) layers (Woodbury et al, 2000) and consists of heterogeneous populations of excitatory and inhibitory interneurons (Millan, 1999). Interneuron subtypes can be distinguished on the basis of morphology, neurotransmitter content, and receptor expression (Millan, 1999).…”

Section: Introductionmentioning

confidence: 99%

Distinct Populations of Spinal Cord Lamina II Interneurons Expressing G-Protein-Gated Potassium Channels

Marker¹,

Luján²,

Colón

et al. 2006

J. Neurosci.

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Noxious stimuli are sensed and carried to the spinal cord dorsal horn by A␦ and C primary afferent fibers. Some of this input is relayed directly to supraspinal sites by projection neurons, whereas much of the input impinges on a heterogeneous population of interneurons in lamina II. Previously, we demonstrated that G-protein-gated inwardly rectifying potassium (GIRK) channels are expressed in lamina II of the mouse spinal cord and that pharmacologic ablation of spinal GIRK channels selectively blunts the analgesic effect of high but not lower doses of intrathecal -opioid receptor (MOR) agonists. Here, we report that GIRK channels formed by GIRK1 and GIRK2 subunits are found in two large populations of lamina II excitatory interneurons. One population displays relatively large apparent whole-cell capacitances and prominent GIRK-dependent current responses to the MOR agonist [D-Ala 2 ,N-MePhe 4 ,Gly-ol 5 ]-enkephalin (DAMGO). A second population shows smaller apparent capacitance values and a GIRK-dependent response to the GABA B receptor agonist baclofen, but not DAMGO. Ultrastructural analysis revealed that GIRK subunits preferentially label type I synaptic glomeruli, suggesting that GIRK-containing lamina II interneurons receive prominent input from C fibers, while receiving little input from A␦ fibers. Thus, excitatory interneurons in lamina II of the mouse spinal cord can be subdivided into different populations based on the neurotransmitter system coupled to GIRK channels. This important distinction will afford a unique opportunity to characterize spinal nociceptive circuitry with defined physiological significance.

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The tracts, cytoarchitecture, and neurochemistry of the spinal cord

Tan

Faull

Curtis

2022

The Anatomical Record

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The human spinal cord can be described using a range of nomenclatures with each providing insight into its structure and function. Here we have comprehensively reviewed the key literature detailing the general structure, configuration of tracts, the cytoarchitecture of Rexed's laminae, and the neurochemistry at the spinal segmental level. The purpose of this review is to detail current anatomical understanding of how the spinal cord is structured and to aid researchers in identifying gaps in the literature that need to be studied to improve our knowledge of the spinal cord which in turn will improve the potential of therapeutic intervention for disorders of the spinal cord.

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On the problem of lamination in the superficial dorsal horn of mammals: A reappraisal of the substantia gelatinosa in postnatal life

Cited by 81 publications

References 81 publications

Myelinated Skin Sensory Neurons Project Extensively throughout Adult Mouse Substantia Gelatinosa

Myelinated Skin Sensory Neurons Project Extensively throughout Adult Mouse Substantia Gelatinosa

Distinct Populations of Spinal Cord Lamina II Interneurons Expressing G-Protein-Gated Potassium Channels

The tracts, cytoarchitecture, and neurochemistry of the spinal cord

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