On the photocatalytic reduction of MTT tetrazolium salt on the surface of TiO2 nanoparticles: Formazan production kinetics and mechanism

Popescu, Traian; Lupu, Andreea-Roxana; Rădiţoiu, Valentin; Purcar, Violeta; Teodorescu, V. S.

doi:10.1016/j.jcis.2015.07.005

Cited by 15 publications

(14 citation statements)

References 69 publications

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“…After the 24 h treatment period, the toxicity of the samples on each cell line was quantified. To achieve this, the medium in each well was replaced by MTT solution (500 μg/mL in medium) and incubated for 2 h. Viability of cells was assessed by their ability to reduce the yellow dye MTT to a blue formazan product [35]. The MTT reagent was removed, the formazan crystals were dissolved in isopropanol, and the absorbance at 560 nm determined using a microplate reader (CLARIO Star Microplate Reader, BMG Labtech, UK).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic Stilbenes and Canthinone Alkaloids from Brucea antidysenterica (Simaroubaceae)

et al. 2019

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A phytochemical study of the root and bark of Brucea antidysenterica J. F. Mill. (Simaroubaceae) afforded three new compounds, including a stilbene glycoside bruceanoside A (1), and two canthinone alkaloids bruceacanthinones A (3) and B (4), along with ten known secondary metabolites, rhaponticin (2), 1,11-dimethoxycanthin-6-one (5), canthin-6-one (6), 1-methoxycanthin-6-one (7), 2-methoxycanthin-6-one (8), 2-hydroxy-1,11-dimethoxycanthin-6-one (9), β-carboline-1-propionic acid (10), cleomiscosin C (11), cleomiscosin A (12), and hydnocarpin (13). The structures of all the compounds were determined using spectrometric and spectroscopic methods including 1D and 2D NMR, and HRSEIMS. The identities of the known compounds were further confirmed by comparison of their data with those reported in the literature. The root and bark methanolic extracts, the dichloromethane and ethyl acetate soluble fractions, and the isolated compounds (3–13), were assessed for their cytotoxicity against the cancer cell lines A-549, MCF-7, and PC-3. The results suggested that compounds in the extracts might possess a synergic action in their cytotoxicity.

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Section: Methodsmentioning

confidence: 99%

Cytotoxic Stilbenes and Canthinone Alkaloids from Brucea antidysenterica (Simaroubaceae)

et al. 2019

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“…After the 24-h treatment period, the toxicity of the samples on each cell line was quantified. To achieve this, the medium in each well was replaced by MTT solution (500 μg/mL in medium) and incubated for 2 h. Viability of cells was assessed by their ability to reduce the yellow dye MTT to a blue formazan product (Popescu, Lupu, Raditoiu, et al, 2015). The MTT reagent was removed, the formazan crystals were dissolved in isopropanol and the absorbance at 560 nm determined using a microplate reader (CLARIO Star Microplate Reader, BMG Labtech, UK).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic Properties of the Stem Bark ofCitrus reticulataBlanco (Rutaceae)

et al. 2017

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The bioassay-guided fractionation of the n-hexane extract of Citrus reticulata Blanco (Rutaceae) stem bark yielded scoparone (1), xanthyletin (2), lupeol (3), β-amyrin (4), stigmasterol (5), β-sitosterol (6) and palmitic acid. The structures of these compounds were determined by comprehensive spectroscopic analyses, i.e., 1D and 2D NMR and EI-MS, and by comparison with the reported data. Extracts, fractions and isolated compounds 1-6 were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-dphenyltetrazolium bromide (MTT) assay against three human cancer cell lines, i.e., human lung adenocarcinoma cell line A549, human breast adenocarcinoma cell line MCF7 and human Caucasian prostate adenocarcinoma cell line PC3. Significant activity of the n-hexane and the dichloromethane extracts was observed against the breast cancer cell line MCF7 with IC s of 45.6 and 54.7 μg/mL, respectively. Moreover, the 70% ethyl acetate in n-hexane chromatographic fraction showed significant activity displaying IC values of 53.0, 52.4 and 49.1 μg/mL against the cancer cell lines A549, MCF7 and PC3, respectively. Encouragingly, an IC of 510.0 μg/mL against the human normal prostate cell line PNT2 indicated very low toxicity and hence favourable selectivity indices for the 70% ethyl acetate in n-hexane fraction in the range of 9.6-10.4 towards cell lines A549, MCF7 and PC3. Because compounds isolated from the above fraction only delivered IC values in the range of 18.2-96.3, 9.2-34.1 and 7.5-97.2 μg/mL against A549, MCF7 and PC3 cell lines, respectively, synergistic action between compounds is suggested. Bioassay results valorize the anticancer effectivity of the stem bark of this plant in Cameroonian pharmacopoeia. Copyright © 2017 John Wiley & Sons, Ltd.

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“…In addition, the number and activity of mitochondria in the tested cells are important, especially when discussing the comparative effect of materials on different cell lines or depending on the stage of cell differentiation [1,67]. In methods in which liquid biological samples containing the compounds of interest are subjected to optical absorbance determinations, the materials possessing a high refractive index, as well as fluorescence and photocatalytic properties, may induce altered results [68,69,70,71,72]. During optical density determinations, by absorbing or reflecting the incident light, suspended nanoparticles may increase the measured absorbance (turbidity/opacity).…”

Section: Biocompatibility Assessment Of Cap-based Bioceramicsmentioning

confidence: 99%

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

et al. 2019

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Recently, a large spectrum of biomaterials emerged, with emphasis on various pure, blended, or doped calcium phosphates (CaPs). Although basic cytocompatibility testing protocols are referred by International Organization for Standardization (ISO) 10993 (parts 1–22), rigorous in vitro testing using cutting-edge technologies should be carried out in order to fully understand the behavior of various biomaterials (whether in bulk or low-dimensional object form) and to better gauge their outcome when implanted. In this review, current molecular techniques are assessed for the in-depth characterization of angiogenic potential, osteogenic capability, and the modulation of oxidative stress and inflammation properties of CaPs and their cation- and/or anion-substituted derivatives. Using such techniques, mechanisms of action of these compounds can be deciphered, highlighting the signaling pathway activation, cross-talk, and modulation by microRNA expression, which in turn can safely pave the road toward a better filtering of the truly functional, application-ready innovative therapeutic bioceramic-based solutions.

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On the photocatalytic reduction of MTT tetrazolium salt on the surface of TiO2 nanoparticles: Formazan production kinetics and mechanism

Cited by 15 publications

References 69 publications

Cytotoxic Stilbenes and Canthinone Alkaloids from Brucea antidysenterica (Simaroubaceae)

Cytotoxic Stilbenes and Canthinone Alkaloids from Brucea antidysenterica (Simaroubaceae)

Cytotoxic Properties of the Stem Bark ofCitrus reticulataBlanco (Rutaceae)

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

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