On the mutagenicity of homologous recombination and double-strand break repair in bacteriophage

Shcherbakov, Victor P.; Plugina, Lidiya; Shcherbakova, Tamara; Sizova, Svetlana; Kudryashova, Elena

doi:10.1016/j.dnarep.2010.09.006

Cited by 2 publications

(2 citation statements)

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“…Besides the probability that recombinant viruses might experience elevated degrees of positive selection favouring the restoration of recombinationally disrupted intra-genome interactions (a process that might be associated with increased numbers of detected mutation events), it is also plausible that the recombination process itself might be inherently mutagenic [31,32]. We therefore compared the mutation rates of the laboratory constructed recombinant, VWMPCPLIRMat, and recombinant MSV genomes [47], with those of the wt MSV-MatA and MSV-VW genomes.…”

Section: Resultsmentioning

confidence: 99%

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Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus

et al. 2012

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BackgroundSingle-stranded (ss) DNA viruses in the family Geminiviridae are proving to be very useful in real-time evolution studies. The high mutation rate of geminiviruses and other ssDNA viruses is somewhat mysterious in that their DNA genomes are replicated in host nuclei by high fidelity host polymerases. Although strand specific mutation biases observed in virus species from the geminivirus genus Mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational processes that operate specifically on ssDNA, it is currently unknown whether viruses from other genera display similar strand specific mutation biases. Also, geminivirus genomes frequently recombine with one another and an alternative cause of their high mutation rates could be that the recombination process is either directly mutagenic or produces a selective environment in which the survival of mutants is favoured. To investigate whether there is an association between recombination and increased basal mutation rates or increased degrees of selection favoring the survival of mutations, we compared the mutation dynamics of the MSV-MatA and MSV-VW field isolates of Maize streak virus (MSV; Mastrevirus), with both a laboratory constructed MSV recombinant, and MSV recombinants closely resembling MSV-MatA. To determine whether strand specific mutation biases are a general characteristic of geminivirus evolution we compared mutation spectra arising during these MSV experiments with those arising during similar experiments involving the geminivirus Tomato yellow leaf curl virus (Begomovirus genus).ResultsAlthough both the genomic distribution of mutations and the occurrence of various convergent mutations at specific genomic sites indicated that either mutation hotspots or selection for adaptive mutations might elevate observed mutation rates in MSV, we found no association between recombination and mutation rates. Importantly, when comparing the mutation spectra of MSV and TYLCV we observed similar strand specific mutation biases arising predominantly from imbalances in the complementary mutations G → T: C → A.ConclusionsWhile our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state.

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Section: Resultsmentioning

confidence: 99%

“…Another factor potentially influencing both the substitution and mutation rates of geminiviruses is genetic recombination, which in some instances has been shown to be mutagenic [30-32]. The possible interplay between mutation, substitution and recombination rates is particularly interesting when considering the geminiviruses.…”

Section: Introductionmentioning

confidence: 99%