On the location of glutamate decarboxylase in the caryopsis of hard wheat (Triticum durum) and its activity during early germination

Succinic semialdehyde dehydrogenase (EC 1.2.1.16) was purified 74-fold from wheat grain (Triticum durum Desf.). The enzyme appears quite specific for succinic semialdehyde (SSA). Both NAD and NADP support the oxidation of the substrate, but the former is 7-fold more active than the latter. The optimum pH for activity is around 9; the enzyme is stable in the pH range 6-9 and retains its whole activity up to 40°C. The enzyme activity is strongly dependent on the presence of mercaptoethanol, other thiol compounds being much less effective. Kinetic data support the formation of a ternary complex between enzyme, substrate and coenzyme. The K m for SSA and for NAD are 7.4x10(-6) M and 2x10(-4) M, respectively. The molecular weight of the enzyme protein was estimated by gel-filtration to be about 130,000.

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Succinic semialdehyde dehydrogenase of wheat grain

Galleschi

Tozzi

Cozzani

et al. 1978

Planta

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Variation of glutamate decarboxylase activity andγ-amino butyric acid content of wheat embryos during ripening of seeds

1977

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GAD activity and gamma-ABA content of wheat embryos at 7 ripening stages were verified with the aim of studying the metabolic activity of embryo during dehydration and quiescence of caryopsis. Data showed that in the early stage of ripening GAD activity is very low, increases rapidly at dough-stage, remaining constant up to waxy-stage, and decreases in the last fully-ripe embryos. gamma-ABA content appears to be roughly parallel to the variations of GAD activity.

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The γ-aminobutyric acid shunt in germinating Sinapis alba seeds

Vandewalle

Olsson

1983

Plant Science Letters

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On the location of glutamate decarboxylase in the caryopsis of hard wheat (Triticum durum) and its activity during early germination

Cited by 5 publications

References 6 publications

Succinic semialdehyde dehydrogenase of wheat grain

Succinic semialdehyde dehydrogenase of wheat grain

Variation of glutamate decarboxylase activity andγ-amino butyric acid content of wheat embryos during ripening of seeds

The γ-aminobutyric acid shunt in germinating Sinapis alba seeds

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