On the inactivity of Candida antartica lipase B towards strong acids

Green Chemistry in der Schule

“…Alternativ lassen sich mit Lipase analog zur säurekatalysierten Veresterung auch „Fruchtester“ synthetisieren. Hierbei sollte man allerdings darauf achten, dass die verwendete Carbonsäure nicht zu sauer ist, da sie sonst die Lipase inaktiviert . So lässt sich Ethyllactat in tert ‐butyl‐Alkohol unter kontrollierten Bedingungen synthetisieren.…”

Molecular Modeling Approach to Enzymatic Polymerization

“…This can be understood in the light of a recent publication by Hollmann et al . [16] who could demonstrate that organic acids having a p K a value of < 4.8 irreversibly inhibit CALB, presumably by protonation of His 224 , which in turn prevents the deprotonation of Ser 105 necessary as part of the catalytic process. β -Alanine has a fi rst p K a of 3.6 [17] which clearly is below the critical value.…”

A novel lipase-based stationary phase in liquid chromatography

“…Novozyme 435, which consists of CALB physically adsorbed onto macroporous acrylic polymer resin [29], is a commercial enzyme product being used successfully and widely. Various factors affecting the activity of CALB have been investigated [30][31][32].…”