On the importance of the supporting material for activity of immobilized Candida antarctica lipase B in ionic liquid/hexane and ionic liquid/supercritical carbon dioxide biphasic media

Lozano, Pedro; Diego, Teresa De; Sauer, Tanja; Vaultier, Michel; Gmouh, Saïd; Iborra, José L.

doi:10.1016/j.supflu.2006.03.025

Cited by 75 publications

(45 citation statements)

References 26 publications

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“…For instance, Contesini and Oliveira [85] N] are not soluble in hexane, so mass-transfer limitations were introduced in the liquid/liquid biphasic system of their mixture. Moreover, the authors stated that the homogeneous distribution of the enzyme onto a support with preferential enzyme-surface interactions and at an optimal hydration level were crucial for the enzyme activity, indicating that a suitable water content in the enzyme microenvironment was essential for the retaining of the native structure of the enzyme and therefore its activity [70,71,86].…”

Section: Biocatalysis In Mixture Solvents Of Organic Solvent and Ionimentioning

confidence: 99%

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

et al. 2016

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Abstract:Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water-dependent side reactions. It has been demonstrated that enzyme has high activity and stability in non-aqueous media, and the variation of enzyme activity is attributed to its conformational modifications. This review comprehensively addresses the stability and activity of the intact enzymes in various non-aqueous systems, such as organic solvents, ionic liquids, sub-/super-critical fluids and their combined mixtures. It has been revealed that critical factors such as Log P, functional groups and the molecular structures of the solvents define the microenvironment surrounding the enzyme molecule and affect enzyme tertiary and secondary structure, influencing enzyme catalytic properties. Therefore, it is of high importance for biocatalysis in non-aqueous media to elucidate the links between the microenvironment surrounding enzyme surface and its stability and activity. In fact, a better understanding of the correlation between different non-aqueous environments and enzyme structure, stability and activity can contribute to identifying the most suitable reaction medium for a given biotransformation.

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Section: Biocatalysis In Mixture Solvents Of Organic Solvent and Ionimentioning

confidence: 99%

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

et al. 2016

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“…In some cases, supercritical fluids have been used in some reactions, and the combined use with ionic liquids has some advantages, improving lipase performance in this system [73][74][75].…”

Section: Pds Coated With Ionic Liquidsmentioning

confidence: 99%

Immobilization of Proteins in Poly-Styrene-Divinylbenzene Matrices: Functional Properties and Applications

Rafael¹,

Hernández²,

Barbosa³

et al. 2015

COC

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Supports based on poly-styrene-divinylbenzene (PSD) are commercially available since a long time ago. However, they are not commonly used as enzyme immobilization matrices. The main reason for this lies in the negative effect of the very hydrophobic surface on enzyme stability that produces the instantaneous enzyme inactivation in many instances. However, they have recently regained some impact on enzyme immobilization. They are easy to modify, and have been prepared with different modifiers. We will pay special attention to the coating of these supports with ionic liquids, which permits to have the ionic liquid phase anchored to the solid and modulate the enzyme properties without risk of losing these expensive and potentially toxic compounds. Thus, this review will present the covalent or physical immobilization of enzymes on PSD supports, submitted to different modifications. Moreover, lipases immobilized via interfacial activation on some naked PSD supports have shown some unexpected improvement in their catalytic properties, with uses in reactions like hydrolysis, esterification or transesterification.

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“…ILs can absorb large quantities of CO 2 at low pressure (0.6 mol fraction at 10 MPa), although the amount of IL dissolved in CO 2 is negligible. This fact not only shows the exceptional ability of SC CO 2 to extract a wide variety of hydrophophic compounds from ILs, but also decreases the viscosity of ILs, thus, improving the mass-transfer phenomena (Lozano et al, 2007b). Applying enzymes in SC CO 2 /ILs biphasic systems has a very short history.…”

Section: An Overview Of Biocatalysis In Ils and In Scfs/ils Systemsmentioning

confidence: 99%

“…The formation of undesired (S)-1-phenylethyl propionate was obtained in low levels, which could be related to increased mass-transfer limitations for substrates and products in SC CO 2 . A commercial solution of free CALB (Novozym 525L) was immobilized onto 12 different silica supports modified with specific side chains, which were assayed for the kinetic resolution of rac-1-phenylethanol in both IL/hexane and SC CO 2 /IL biphasic media (Lozano, et al, 2007b ]) improved the synthetic activity of the enzyme in SC CO 2 by up to 6 times with respect to the hexane medium. This could be due to the excellent ability of SC CO 2 to transport dissolved solutes through the IL phase, which improves the transfer rate of substrates to the enzyme microenvironment compared with the liquid systems.…”

Section: An Overview Of Biocatalysis In Ils and In Scfs/ils Systemsmentioning

confidence: 99%