On the Effect of Soft Molecularly Imprinted Nanoparticles Receptors Combined to Nanoplasmonic Probes for Biomedical Applications

Cennamo, Nunzio; Bossi, Alessandra Maria; Arcadio, Francesco; Maniglio, Devid; Zeni, Luigi

doi:10.3389/fbioe.2021.801489

Cited by 15 publications

(11 citation statements)

References 71 publications

(107 reference statements)

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“…Here, we explored for the first time the effect of coupling nanogels to a POF plasmonic probe as a modification aspect of a state-of-the-art coupling of SPR POF probes with molecularly imprinted nanoparticles (nanoMIPs), synthetic polymeric receptors optimized for recognizing a target analyte using template-assisted synthesis and [15][16][17].…”

Section: Discussionmentioning

confidence: 99%

“…Nanogels were coupled to the POF, as described in [16]. A SAM was formed by incubating the gold POF surface in a 300 µM (R)-(+)-α-lipoic acid in ethanol 8% v/v solution overnight.…”

Section: Preparation Of the Nanogel-pof Platformmentioning

confidence: 99%

“…Thermo-and solvent-responsive hydrogels have been demonstrated to provide improved optical sensitivity [14]. Substantial enhancement in sensitivity has also been achieved using soft molecularly imprinted nanogels if/when coupled to plasmonics [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Non-Specific Responsive Nanogels and Plasmonics to Design MathMaterial Sensing Interfaces: The Case of a Solvent Sensor

Cennamo

Arcadio

Capasso

et al. 2022

Sensors

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The combination of non-specific deformable nanogels and plasmonic optical probes provides an innovative solution for specific sensing using a generalistic recognition layer. Soft polyacrylamide nanogels that lack specific selectivity but are characterized by responsive behavior, i.e., shrinking and swelling dependent on the surrounding environment, were grafted to a gold plasmonic D-shaped plastic optical fiber (POF) probe. The nanogel–POF cyclically challenged with water or alcoholic solutions optically reported the reversible solvent-to-phase transitions of the nanomaterial, embodying a primary optical switch. Additionally, the non-specific nanogel–POF interface exhibited more degrees of freedom through which specific sensing was enabled. The real-time monitoring of the refractive index variations due to the time-related volume-to-phase transition effects of the nanogels enabled us to determine the environment’s characteristics and broadly classify solvents. Hence the nanogel–POF interface was a descriptor of mathematical functions for substance identification and classification processes. These results epitomize the concept of responsive non-specific nanomaterials to perform a multiparametric description of the environment, offering a specific set of features for the processing stage and particularly suitable for machine and deep learning. Thus, soft MathMaterial interfaces provide the ground to devise devices suitable for the next generation of smart intelligent sensing processes.

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Section: Discussionmentioning

confidence: 99%

“…Nanogels were coupled to the POF, as described in [16]. A SAM was formed by incubating the gold POF surface in a 300 µM (R)-(+)-α-lipoic acid in ethanol 8% v/v solution overnight.…”

Section: Preparation Of the Nanogel-pof Platformmentioning

confidence: 99%

See 1 more Smart Citation

Non-Specific Responsive Nanogels and Plasmonics to Design MathMaterial Sensing Interfaces: The Case of a Solvent Sensor

Cennamo

Arcadio

Capasso

et al. 2022

Sensors

Self Cite

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“…Although the sensing systems were successfully applied for detection of HTR in (10 6 times) diluted serum mimic and human serum, 72 more applied work of developed plasmonic devices operating in complex biofluids is needed. Still, these works demonstrated that soft nanoMIPs can be promising receptors for improved protein analysis by active plasmonics 72,73,98 or MALDI-TOF-MS 99 and can bring in new opportunities to the MIP biosensing field. The scientific community is currently making efforts to come up with new synthetic procedures to prepare nanocomposites with efficient Raman enhancement.…”

Section: ■ Epitope Imprintingmentioning

confidence: 99%

Imprinted Hydrogel Nanoparticles for Protein Biosensing: A Review

et al. 2023

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Over the past decade, molecular imprinting (MI) technology has made tremendous progress, and the advancements in nanotechnology have been the major driving force behind the improvement of MI technology. The preparation of nanoscale imprinted materials, i.e., molecularly imprinted polymer nanoparticles (MIP NPs, also commonly called nanoMIPs), opened new horizons in terms of practical applications, including in the field of sensors. Currently, hydrogels are very promising for applications in bioanalytical assays and sensors due to their high biocompatibility and possibility to tune chemical composition, size (microgels, nanogels, etc.), and format (nanostructures, MIP film, fibers, etc.) to prepare optimized analyte-responsive imprinted materials. This review aims to highlight the recent progress on the use of hydrogel MIP NPs for biosensing purposes over the past decade, mainly focusing on their incorporation on sensing devices for detection of a fundamental class of biomolecules, the peptides and proteins. The review begins by directing its focus on the ability of MIPs to replace biological antibodies in (bio)analytical assays and highlight their great potential to face the current demands of chemical sensing in several fields, such as disease diagnosis, food safety, environmental monitoring, among others. After that, we address the general advantages of nanosized MIPs over macro/micro-MIP materials, such as higher affinity toward target analytes and improved binding kinetics. Then, we provide a general overview on hydrogel properties and their great advantages for applications in the field of Sensors, followed by a brief description on current popular routes for synthesis of imprinted hydrogel nanospheres targeting large biomolecules, namely precipitation polymerization and solid-phase synthesis, along with fruitful combination with epitope imprinting as reliable approaches for developing optimized protein-imprinted materials. In the second part of the review, we have provided the state of the art on the application of MIP nanogels for screening macromolecules with sensors having different transduction modes (optical, electrochemical, thermal, etc.) and design formats for single use, reusable, continuous monitoring, and even multiple analyte detection in specialized laboratories or in situ using mobile technology. Finally, we explore aspects about the development of this technology and its applications and discuss areas of future growth.

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“…In the past, researchers have exploited other advanced techniques to address different drawbacks of NAT-and molecular-based detection methods. These techniques include electrochemical [9,10], quartz crystal microbalance (QCM) [11,12], surface acoustic waves (SAW) [13], surface plasmon resonance/imaging (SPR(i)) [14,15], optical fiber [16], and surface-enhanced Raman spectroscopy (SERS) [17] that have been developed for the detection of viral infections. Table 1 also lists the existing sensor methods for COVID-19 diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Molecularly Imprinted Polymer Nanoparticles for SARS-CoV-2 Virus Detection Using Surface Plasmon Resonance

et al. 2022

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COVID-19 caused by a SARS-CoV-2 infection was first reported from Wuhan, China, and later recognized as a pandemic on March 11, 2020, by the World Health Organization (WHO). Gold standard nucleic acid and molecular-based testing have largely satisfied the requirements of early diagnosis and management of this infectious disease; however, these techniques are expensive and not readily available for point-of-care (POC) applications. The COVID-19 pandemic of the 21st century has emphasized that medicine is in dire need of advanced, rapid, and cheap diagnostic tools. Herein, we report on molecularly imprinted polymer nanoparticles (MIP-NPs/nanoMIPs) as plastic antibodies for the specific detection of SARS-CoV-2 by employing a surface plasmon resonance (SPR) sensor. High-affinity MIP-NPs directed against SARS-CoV-2 were manufactured using a solid-phase imprinting method. The MIP-NPs were then characterized using dynamic light scattering (DLS) and atomic force microscopy (AFM) prior to their incorporation into a label-free portable SPR device. Detection of SARS-CoV-2 was studied within a range of 104–106 PFU mL−1. The MIP-NPs demonstrated good binding affinity (KD = 0.12 pM) and selectivity toward SARS-CoV-2. The AFM, cyclic voltammetry, and square-wave voltammetry studies revealed the successful stepwise preparation of the sensor. A cross-reactivity test confirmed the specificity of the sensor. For the first time, this study demonstrates the potential of molecular imprinting technology in conjunction with miniaturized SPR devices for the detection of SARS-CoV-2 particles with high-affinity and specificity. Such sensors could help monitor and manage the risks related to virus contamination and infections also beyond the current pandemic.

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On the Effect of Soft Molecularly Imprinted Nanoparticles Receptors Combined to Nanoplasmonic Probes for Biomedical Applications

Cited by 15 publications

References 71 publications

Non-Specific Responsive Nanogels and Plasmonics to Design MathMaterial Sensing Interfaces: The Case of a Solvent Sensor

Non-Specific Responsive Nanogels and Plasmonics to Design MathMaterial Sensing Interfaces: The Case of a Solvent Sensor

Imprinted Hydrogel Nanoparticles for Protein Biosensing: A Review

Synthesis of Molecularly Imprinted Polymer Nanoparticles for SARS-CoV-2 Virus Detection Using Surface Plasmon Resonance

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