On-Site Colorimetric Detection of Cholesterol Based on Polypyrrole Nanoparticles

Hong, Chengyi; Zhang, Xiaoxia; Wu, Chengzhi; Chen, Qin; Yang, Hongfen; Yang, Dan; Huang, Zhiyong; Cai, Ren; Tan, Weihong

doi:10.1021/acsami.0c15900

Cited by 95 publications

(52 citation statements)

References 36 publications

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“…The human serum sample was prediluted 150‐fold with PBS (10 mmol L −1 , pH 7) containing triton X‐100 and isopropanol to avoid matrix effect. [ 86 ] First, the cholesterol concentration in diluted unspiked serum sample was determined using a commercial kit to be 0.03 mmol L −1 . Then spiked cholesterol concentrations (0.3, 2, and 5 mmol L −1 ) were prepared by adding standard cholesterol in the diluted human serum sample.…”

Section: Methodsmentioning

confidence: 99%

L‐Cysteine‐Meditated Self‐Assembled PtRu Derived Bimetallic Metal–Carbon Hybrid: An Excellent Peroxidase Mimics for Colorimetric and Fluorometric Detection of Hydrogen Peroxide and Cholesterol

Gupta

Son

Seong

2021

Adv Materials Inter

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Abnormal cholesterol concentrations are related to dyslipidemia, cardiovascular disease, anemia, hypothyroidism, cerebral hemorrhages, and cancer. [2] The primary sources of human cholesterol are diet and synthesis; thus, cholesterol levels can represent the diet and serum spectrum, and are important in food quality control and clinical diagnosing. Various methods, such as colorimetric, enzymatic, fluorometric, chromatographic, and electrochemical approaches, have been proposed for cholesterol quantification. [3,4] Among those methods, indirect cholesterol quantification through an optical analysis of the hydrogen peroxide (H 2 O 2 ) produced by cholesterol oxidation catalyzed by cholesterol oxidase (ChO x ) has proved to be popular and effective. [5,6] In the human body, H 2 O 2 production occurs in response to various enzymatic reactions, and thus it has been suggested as a potential biomarker. [7] Apart from its well-known cytotoxic effects, H 2 O 2 plays a crucial role in many physiological and biological processes, and has significant applications in textiles, cosmetics, agriculture, heavy industry, room decontamination, and food processing. [8] The conventional optical detection methods use natural enzymes (such as horseradish peroxide, HRP), which catalyze H 2 O 2 and produce hydroxyl radicals that oxidize specific substrates and generate readable signals. [3,[9][10][11][12] Although natural enzymes have the advantages of high sensitivity and selectivity, they suffer from complicated immobilization mechanisms, high costs, slow catalytic activity, and low reliability under harsh environmental conditions. [13] When they are used in sensing methods, natural enzymes are particularly vulnerable to interfering substances. [13,14] Therefore, the use of artificial enzymes has gained considerable interest because of their ability to overcome the shortcomings of natural enzymes. [15][16][17][18][19][20][21][22] Since the first artificial enzyme (Fe 3 O 4 ) was discovered, a range of materials has been found to have intrinsic enzymelike activity, including noble metals, transition metals/metal oxides, carbon variants, composites, and metal-organic frameworks. [23][24][25][26] Nanozymes based on metal-carbon hybrids have proven to be particularly effective. [27] Bao et. al. prepared a Artificial enzymes have attracted considerable attention because of their significant advantages over natural enzymes. Herein, the authors report a simple hydrothermal strategy for producing a bimetallic metal -N-, S-doped carbon (NS-C@PtRu) hybrid derived from L-cysteine-mediated self-assembly of PtRu. The peroxidase-like activity and colloidal stability of the NS-C@PtRu are tuned by varying the amount of L-cysteine, the source material for the N-, S-doped carbon. The NS-C@PtRu exhibits good dispersibility, synergistic interactions (metal-metal, metal-carbon), non-metal (N, S) doping, more catalytic active sites, improved noble metallic catalyst performance, and high utilization efficiency. As an excellent peroxidase mimic, NS-C@PtRu ca...

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Section: Methodsmentioning

confidence: 99%

L‐Cysteine‐Meditated Self‐Assembled PtRu Derived Bimetallic Metal–Carbon Hybrid: An Excellent Peroxidase Mimics for Colorimetric and Fluorometric Detection of Hydrogen Peroxide and Cholesterol

Gupta

Son

Seong

2021

Adv Materials Inter

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“…Numerous efforts have been applied to integrating nanomaterials with analytical chemical approaches for the accurate quantitation of targeted metabolite biomarkers. [ 94–105 ]…”

Section: The Application Of Metabolic Analysis In Ivdmentioning

confidence: 99%

Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics

2022

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In vitro diagnostics (IVD) has played an indispensable role in healthcare system by providing necessary information to indicate disease condition and guide therapeutic decision. Metabolic analysis can be the primary choice to facilitate the IVD since it characterizes the downstream metabolites and offers real‐time feedback of the human body. Nanomaterials with well‐designed composition and nanostructure have been developed for the construction of high‐performance detection platforms toward metabolic analysis. Herein, we summarize the recent progress of nanomaterials‐assisted metabolic analysis and the related applications in IVD. We first introduce the important role that nanomaterials play in metabolic analysis when coupled with different detection platforms, including electrochemical sensors, optical spectrometry, and mass spectrometry. We further highlight the nanomaterials‐assisted metabolic analysis toward IVD applications, from the perspectives of both the targeted biomarker quantitation and untargeted fingerprint extraction. This review provides fundamental insights into the function of nanomaterials in metabolic analysis, thus facilitating the design of next‐generation diagnostic devices in clinical practice.

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“…In this regard, various methods of identifying and preparing samples for the analysis of tetracycline residues in food products are being developed. A highly selective method for the determination of the tetracycline traces in food is presented in the article [20]. The authors of the article prepared an aptamer attached to the tetrahedral nanostructured functionalized DNA magnetic beads (Apt-tet MB) as a probe to detect tetracycline.…”

Section: Tetracyclinesmentioning

confidence: 99%

Untitled

2022

J. Med. Chem. Sci.

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Due to the widespread use of veterinary drugs and antibiotics in animal husbandry, one of the most critical problems in ensuring food safety is the presence of residual antibiotic traces in animal products. Concentrations of such traces in food products pose a danger to both humans and the environment as a whole. Irrational use of antibiotics in agriculture stimulates resistant bacteria that can cause infectious diseases in humans and animals that cannot be treated with modern drugs. Due to the potential risk to human health in many countries, maximum permissible limits for the content of residual traces of antibiotics are regulated. This review aimed at investigating the recent publications in the field of identification of residual antibiotic traces in food. Various types of high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) combined with solid-phase extraction were mainly used for the quantitative determination of analytes. Methods of enzyme immunoassay, capillary electrophoresis, and infrared spectroscopy with Fourier transform, biosensor, and fluorescence analysis methods are also presented. It is shown that the development of new susceptible, accurate, simple, and cost-effective methods for the determination traces of antibiotics in food remains an urgent task and is highly demanded in the modern world.

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On-Site Colorimetric Detection of Cholesterol Based on Polypyrrole Nanoparticles

Cited by 95 publications

References 36 publications

L‐Cysteine‐Meditated Self‐Assembled PtRu Derived Bimetallic Metal–Carbon Hybrid: An Excellent Peroxidase Mimics for Colorimetric and Fluorometric Detection of Hydrogen Peroxide and Cholesterol

L‐Cysteine‐Meditated Self‐Assembled PtRu Derived Bimetallic Metal–Carbon Hybrid: An Excellent Peroxidase Mimics for Colorimetric and Fluorometric Detection of Hydrogen Peroxide and Cholesterol

Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics

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