On Sample Preparation for Surface-Enhanced Raman Scattering (SERS) of Bacteria and the Source of Spectral Features of the Spectra

Kahraman, Mehmet; Keseroğlu, Kemal; Çulha, Mustafa

doi:10.1366/10-06184

Cited by 66 publications

(76 citation statements)

References 32 publications

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“…The fluorescence quenching is another advantage of the technique to mention. Due to its remarkable and versatile features, this technique has been used heavily for detection and identification of bacteria [15][16][17][18][19][20][21], cells [22], tissues [23], and biomolecules [24] within the last decade. Therefore, SERS has a potential to be used for diagnostic purposes in clinical settings and several studies have been published on the way to realize this goal [25].…”

Section: Introductionmentioning

confidence: 99%

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Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

et al. 2015

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Urinary tract infection (UTI) is a widespread infection and affects millions of people around the globe. The gold standard for identification of microorganisms causing infection is urine culture. However, current methods require at least 24 h for the results. In clinical settings, identification and discrimination of bacteria with less time-consuming and cheaper methods are highly desired. In recent years, the power of surface-enhanced Raman scattering (SERS) for fast identification of bacteria and biomolecules has been demonstrated. In this study, we show discrimination of urinary tract infection causative pathogens within 1 h of incubation using principal component analysis (PCA) of SERS spectra of seven different UTI causative bacterial species. In addition, we showed differentiation of them at their different growth phases. We also analyzed origins of bacterial SERS spectra and demonstrated the highly dynamic structure of the bacteria cell wall during their growth. Graphical Abstract Collection of bacteria from urine sample, and their discrimination using their SERS spectra and multivariate analysis.

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Section: Introductionmentioning

confidence: 99%

“…There is a consensus among bacteria-SERS community that the origins of the spectra are bacteria cell wall components and some metabolites, which reside in the cell wall during spectral acquisition [16,30]. When the effective plasmon field of silver nanoparticles (up to 4 nm) is considered, this is highly logical.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

et al. 2015

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“…Figure 3F shows the SERS spectra of captured S. aureus on the are seen clearly and in accordance with those in previous reports categorized in Table S1. 51,52 The SERS signal becomes weak as the S. aureus concentration decreases. In addition, the major Raman peaks of S. aureus can be observed at the concentration of 10 6 …”

Section: Enrichment Performance and Sers Ability Of Fe 3 O 4 @Ag-van mentioning

confidence: 99%

“…51,52 It is observed that the SERS signal becomes weak as the concentration of bacteria decreases. The strongest peak observed at 733 cm 2 cells/mL) suggests that the low limit of detection of the combined system for S. aureus detection (5×10 2 cells/mL) lies within a large dynamic detection range ( Figure S7).…”

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confidence: 99%

Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria

Wang¹,

Liu³

et al. 2018

IJN

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Background Pathogenic bacteria have always been a significant threat to human health. The detection of pathogens needs to be rapid, accurate, and convenient. Methods We present a sensitive surface-enhanced Raman scattering (SERS) biosensor based on the combination of vancomycin-modified Ag-coated magnetic nanoparticles (Fe 3 O 4 @Ag-Van MNPs) and Au@Ag nanoparticles (NPs) that can effectively capture and discriminate bacterial pathogens from solution. The high-performance Fe 3 O 4 @Ag MNPs were modified with vancomycin and used as bacteria capturer for magnetic separation and enrichment. The modified MNPS were found to exhibit strong affinity with a broad range of Gram-positive and Gram-negative bacteria. After separating and rinsing bacteria, Fe 3 O 4 @Ag-Van MNPs and Au@Ag NPs were synergistically used to construct a very large number of hot spots on bacteria cells, leading to ultrasensitive SERS detection. Results The dominant merits of our dual enhanced strategy included high bacterial-capture efficiency (>65%) within a wide pH range (pH 3.0–11.0), a short assay time (<30 min), and a low detection limit (5×10 2 cells/mL). Moreover, the spiked tests show that this method is still valid in milk and blood samples. Owing to these capabilities, the combined system enabled the sensitive and specific discrimination of different pathogens in complex solution, as verified by its detection of Gram-positive bacterium Escherichia coli , Gram-positive bacterium Staphylococcus aureus , and methicillin-resistant S. aureus . Conclusion This method has great potential for field applications in food safety, environmental monitoring, and infectious disease diagnosis.

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“…The origin of the bands that appeared on the SERS spectra of bacteria was independently investigated by two groups. Kahraman et al demonstrated that the SERS spectra obtained from a bacterial sample after several washing steps originate from the bacterial structures on the bacteria wall with some contribution from metabolites released during the sample preparation, mixing, and drying [147]. Premasiri et al showed that the bacteria grown in different culture media resulted with the same SERS spectrum indicating the source of spectral features on the spectrum were from bacteria [148].…”

Section: Detection Identification and Characterization Of Biomacrommentioning

confidence: 99%

Surface-Enhanced Raman Scattering as an Emerging Characterization and Detection Technique

Çulha

Cullum

Lavrik

et al. 2012

Journal of Nanotechnology

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While surface-enhanced Raman spectroscopy (SERS) has been attracting a continuously increasing interest of scientific community since its discovery, it has enjoyed a particularly rapid growth in the last decade. Most notable recent advances in SERS include novel technological approaches to SERS substrates and innovative applications of SERS in medicine and molecular biology. While a number of excellent reviews devoted to SERS appeared in the literature over the last two decades, we will focus this paper more specifically on several promising trends that have been highlighted less frequently. In particular, we will briefly overview strategies in designing and fabricating SERS substrates using deterministic patterning and then cover most recent biological applications of SERS.

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On Sample Preparation for Surface-Enhanced Raman Scattering (SERS) of Bacteria and the Source of Spectral Features of the Spectra

Cited by 66 publications

References 32 publications

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

Discrimination of urinary tract infection pathogens by means of their growth profiles using surface enhanced Raman scattering

Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria

Surface-Enhanced Raman Scattering as an Emerging Characterization and Detection Technique

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