On-line sample cleanup and chiral separation of gemifloxacin in a urinary solution using chiral crown ether as a chiral selector in microchip electrophoresis

Cho, Seung Il; Shim, Jiyeon; Kim, Minsu; Kim, Yong-Kweon; Chung, Doo Soo

doi:10.1016/j.chroma.2004.08.124

Cited by 53 publications

(30 citation statements)

References 21 publications

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“…Thus, MCE has been applied for electrophoretic separation for a variety of biochemical and chemical applications [6,7] since it was introduced by Manz's group [8]. Particularly, enantiomeric separations on MCE have drawn much attention recently [9][10][11][12][13][14][15][16][17][18][19][20][21][22]. Among these, CDs and their derivatives were basically used as chiral selectors, and these chiral additives were dissolved in the electrolyte as the so-called pseudostationary phases.…”

Section: Introductionmentioning

confidence: 99%

On‐chip chiral separation based on bovine serum albumin‐conjugated carbon nanotubes as stationary phase in a microchannel

Weng

Liu

et al. 2006

Electrophoresis

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A novel method of chiral separation based on protein-stationary phase immobilized in a poly(methyl methacrylate) microfluidic chip was developed. BSA conjugated with the shortened carboxylic single-walled carbon nanotubes (SWNTs) was employed as the chiral selector. Successful separation of tryptophan enantiomers was achieved in less than 70 s with a resolution factor of 1.35 utilizing a separation length of 32 mm. This is the first example of chiral separation based on SWNTs-BSA conjugates as stationary phase immobilized in microchip channel. The stability of the stationary phase in the channel was examined by microchip electrophoresis with laser-induced fluorescence detection. Factors that influenced the chiral separation resolution were examined. Under the optimized conditions, the proposed modified chip revealed adequate repeatability concerning run-to-run. These results show that the use of SWNTs-BSA conjugates within microfluidic channels hold great promise for a variety of analytical schemes.

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Section: Introductionmentioning

confidence: 99%

On‐chip chiral separation based on bovine serum albumin‐conjugated carbon nanotubes as stationary phase in a microchannel

Weng

Liu

et al. 2006

Electrophoresis

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“…As in classical CE the most commonly used chiral selector are the CD derivatives [175][176][177]. However, in the last year also chiral separations with ligand-exchange [178] and crown ether [179] have been described. MCE has great potential for chiral highthroughput screening and also for real-time process control [180].…”

Section: Concluding Remarks and Future Trendsmentioning

confidence: 99%

Chiral separations by capillary electrophoresis: Recent developments and applications

2006

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“…This increases claims on the purity and composition of electrolyte systems and limits some applications. Therefore, to our best knowledge, there is only one application of CWE to the chiral analysis of biological sample from 2000 [97]. 18-Crown-6-tetracarboxylic acid crown ether was applied for enantioseparation of gemifloxacin in urine (for details see Table 1).…”

Section: Crown Ethersmentioning

confidence: 99%

Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples

Mikuš

Maráková

2009

Electrophoresis

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An analysis of recent trends indicates that CE can show real advantages over chromatographic methods in ultratrace enantioselective determination of biologically active compounds in complex biological matrices. It is due to high separation efficiency and many applicable in-capillary electromigration effects in CE (countercurrent migration, stacking effects) enhancing significantly (enantio)separability and enabling effective sample preparation (preconcentration, purification, analyte derivatization). Other possible on-line combinations of CE, such as column coupled CE-CE techniques and implementation of nonelectrophoretic techniques (extraction, membrane filtration, flow injection) into CE, offer additional approaches for highly effective sample preparation and separation. CE matured to a highly flexible and compatible technique enabling its hyphenation with powerful detection systems allowing extremely sensitive detection (e.g. LIF) and/or structural characterization of analytes (e.g. MS). Within the last decade, more as well as less conventional analytical on-line approaches have been effectively utilized in this field and their practical potentialities are demonstrated on many new application examples in this article. Here, three basic areas of (enantioselective) drug bioanalysis are highlighted and supported by a brief theoretical description of each individual approach in a compact review structure (to create integrated view on the topic), including (i) progressive enantioseparation approaches and new enantioselective agents, (ii) in-capillary sample preparation (preconcentration, purification, derivatization), and (iii) detection possibilities related to enhanced sensitivity and structural characterization.

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On-line sample cleanup and chiral separation of gemifloxacin in a urinary solution using chiral crown ether as a chiral selector in microchip electrophoresis

Cited by 53 publications

References 21 publications

On‐chip chiral separation based on bovine serum albumin‐conjugated carbon nanotubes as stationary phase in a microchannel

On‐chip chiral separation based on bovine serum albumin‐conjugated carbon nanotubes as stationary phase in a microchannel

Chiral separations by capillary electrophoresis: Recent developments and applications

Advanced CE for chiral analysis of drugs, metabolites, and biomarkers in biological samples

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