2002
DOI: 10.1002/rcm.727
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On‐line on‐chip post‐column derivatisation reactions for pre‐ionisation of analytes and cluster analysis in gradient µ‐liquid chromatography/electrospray mass spectrometry

Abstract: A system is presented that demonstrates the principle of on-line and on-chip post-column derivatisation reactions in micro-high-performance liquid chromatography (micro-HPLC) hyphenated to electrospray time-of-flight mass spectrometry (ESI-TOFMS). In this micro-HPLC-chip-MS set-up, the analytes are separated using gradient micro-HPLC and subsequently derivatised on-chip and detected. One of the major limitations of MS detection is its dependency on the degree of ionisation, which is widely variable and compoun… Show more

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Cited by 20 publications
(22 citation statements)
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“…When using MS detection coupled to microfluidic devices, ESI is the favorable source of ionization [1][2][3] due to atmospheric operation conditions and above all the ability to handle liquid flow rates similar to those in microfluidic systems (nL-mL/min).…”
Section: Introductionmentioning
confidence: 99%
“…When using MS detection coupled to microfluidic devices, ESI is the favorable source of ionization [1][2][3] due to atmospheric operation conditions and above all the ability to handle liquid flow rates similar to those in microfluidic systems (nL-mL/min).…”
Section: Introductionmentioning
confidence: 99%
“…[3,8,[12][13][14][15] Derivatization in urine, however, poses substantial challenges as 55 mmol/day and 64 mmol/day of small organic acids are excreted in women and men, respectively, with Table 1. [3,8,[12][13][14][15] Derivatization in urine, however, poses substantial challenges as 55 mmol/day and 64 mmol/day of small organic acids are excreted in women and men, respectively, with Table 1.…”
Section: Discussionmentioning
confidence: 99%
“…This would not only improve on the selectivity of the method but also improve the sensitivity. There are two approaches to performing integrated labeling on chip: post-and pre-column derivatization [23][24][25][26][27]. Recently, Ro et al [28] have performed pre-column onchip labeling of biogenic amines using the derivatizing agent o-phthaladehyde (OPA) followed by separation by MEKC.…”
Section: Introductionmentioning
confidence: 99%