On-line monitoring of marine cyanobacterial cultivation based on phycocyanïn fluorescence

Sode, Koji; Horikoshi, Kenichi; Takeyama, Haruko; Nakamura, Noriyuki; Matsunaga, Tadashi

doi:10.1016/0168-1656(91)90042-t

Cited by 26 publications

(7 citation statements)

References 12 publications

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“…The 590 nm filter was used for the excitation of phycocyanin, although the excitation maximum of this pigment lies at 620 nm. This wavelength can be used [15], since the excitation peak is relatively broad. Moreover, chlorophylls and carotenoids of eukaryotic algae are weakly excited between 550 nm and 600 nm, while they show secondary excitation peaks in the red part of the spectrum [16].…”

Section: Discussionmentioning

confidence: 99%

A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae

Gregor

Maršálek

2005

Acta hydrochim. hydrobiol.

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A method for the rapid detection of cyanobacteria (blue-green algae) and their differenand Ecotoxicology, Kvě tná 8, tiation from eukaryotic algae in natural phytoplankton assemblages is presented. Fluo-603 65 Brno, Czech Republic rescence emission of photosynthetic pigments at 670 nm was measured using a microplate fluorescence reader when excited at two different wavelengths Ϫ 485 nm and 590 nm. The ratio of fluorescence excited at these wavelengths (590 nm/485 nm) was proportional to the ratio of cyanobacteria and eukaryotic algae, which was determined by the in situ spectrofluorometer for the phytoplankton quantification. The fluorescence intensity was equal to the total chlorophyll-a content. These two fluorescence values can provide the first warning on a development of potentially toxic cyanobacteria in water.

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Section: Discussionmentioning

confidence: 99%

A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae

Gregor

Maršálek

2005

Acta hydrochim. hydrobiol.

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“…Phycocyanin was extracted by incubating freeze-dried samples for 2 h at 37°C in a phosphate buffer (65 mM, pH 8.2) with added lysozyme (15 mg ml −1 ). Subsequently, the lysate was centrifuged at 3000 g for 20 min at 4°C, the supernatant's absorbance was measured in a spectrophotometer (Beckmann, DU640), and the pigment concentration was calculated previously described [87]. Chlorophylls and carotenoid pigments were extracted by overnight incubation of freeze-dried samples in 100% methanol at −20°C.…”

Section: Methodsmentioning

confidence: 99%

The Discovery of Stromatolites Developing at 3570 m above Sea Level in a High-Altitude Volcanic Lake Socompa, Argentinean Andes

et al. 2013

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We describe stromatolites forming at an altitude of 3570 m at the shore of a volcanic lake Socompa, Argentinean Andes. The water at the site of stromatolites formation is alkaline, hypersaline, rich in inorganic nutrients, very rich in arsenic, and warm (20–24°C) due to a hydrothermal input. The stromatolites do not lithify, but form broad, rounded and low-domed bioherms dominated by diatom frustules and aragonite micro-crystals agglutinated by extracellular substances. In comparison to other modern stromatolites, they harbour an atypical microbial community characterized by highly abundant representatives of Deinococcus-Thermus, Rhodobacteraceae, Desulfobacterales and Spirochaetes. Additionally, a high proportion of the sequences that could not be classified at phylum level showed less than 80% identity to the best hit in the NCBI database, suggesting the presence of novel distant lineages. The primary production in the stromatolites is generally high and likely dominated by Microcoleus sp. Through negative phototaxis, the location of these cyanobacteria in the stromatolites is controlled by UV light, which greatly influences their photosynthetic activity. Diatoms, dominated by Amphora sp., are abundant in the anoxic, sulfidic and essentially dark parts of the stromatolites. Although their origin in the stromatolites is unclear, they are possibly an important source of anaerobically degraded organic matter that induces in situ aragonite precipitation. To the best of our knowledge, this is so far the highest altitude with documented actively forming stromatolites. Their generally rich, diverse and to a large extent novel microbial community likely harbours valuable genetic and proteomic reserves, and thus deserves active protection. Furthermore, since the stromatolites flourish in an environment characterized by a multitude of extremes, including high exposure to UV radiation, they can be an excellent model system for studying microbial adaptations under conditions that, at least in part, resemble those during the early phase of life evolution on Earth.

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“…As described previously, also genetically stabilized C-PC fusion proteins fused to biospecific recognition domains have been used directly as biospecific fluorescent probes [17, 71]. The other applications of phycocyanins as fluorescent probe includes the utilization of in vivo fluorescence from phycocyanin for online monitoring of growth in cyanobacterial cultures [101], detection of toxic cyanobacteria in drinking water [102], and remote sensing of cyanobacteria in natural water bodies [103]. Badakere et al [104] and Kulkarni et al [89] reported the possibility of staining RBCs, WBCs, platelets, lymphocytes, nucleated cells, and genomic DNA with C-PC.…”

Section: Applications Of C-phycocyaninmentioning

confidence: 99%

Recent Developments in Production and Biotechnological Applications of C-Phycocyanin

Kuddus

Singh

Thomas

et al. 2013

BioMed Research International

227

122

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An extensive range of pigments including phycobiliproteins are present in algae. C-phycocyanin (C-PC), a phycobiliprotein, is one of the key pigments of Spirulina, a microalgae used in many countries as a dietary supplement. Algal pigments have massive commercial value as natural colorants in nutraceutical, cosmetics, and pharmaceutical industries, besides their health benefits. At present, increasing awareness of harmful effects of synthetic compounds and inclination of community towards the usage of natural products have led to the exploitation of microalgae as a source of natural pigments/colors. This review describes recent findings about the sources and production of C-PC, with emphasis on specific techniques for extraction and purification, along with potential industrial applications in diagnostics, foods, cosmetics, and pharmaceutical industries.

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On-line monitoring of marine cyanobacterial cultivation based on phycocyanïn fluorescence

Cited by 26 publications

References 12 publications

A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae

A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae

The Discovery of Stromatolites Developing at 3570 m above Sea Level in a High-Altitude Volcanic Lake Socompa, Argentinean Andes

Recent Developments in Production and Biotechnological Applications of C-Phycocyanin

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