A Simple In Vivo Fluorescence Method for the Selective Detection and Quantification of Freshwater Cyanobacteria and Eukaryotic Algae

Abstract

A method for the rapid detection of cyanobacteria (blue-green algae) and their differenand Ecotoxicology, Kvě tná 8, tiation from eukaryotic algae in natural phytoplankton assemblages is presented. Fluo-603 65 Brno, Czech Republic rescence emission of photosynthetic pigments at 670 nm was measured using a microplate fluorescence reader when excited at two different wavelengths Ϫ 485 nm and 590 nm. The ratio of fluorescence excited at these wavelengths (590 nm/485 nm) was proportional to the ratio of cyanobacteria and eukaryotic algae, which was determined by the in situ spectrofluorometer for the phytoplankton quantification. The fluorescence intensity was equal to the total chlorophyll-a content. These two fluorescence values can provide the first warning on a development of potentially toxic cyanobacteria in water.

Extraction methods for phycocyanin determination in freshwater filamentous cyanobacteria and their application in a shallow lake

“…The assessment of cyanobacterial presence or dominance with phycocyanin detection is well known: in situ fluorometric field applications (e.g. Seppälä et al, 2007), sensitive fluorometric techniques (Gregor & Maršálek, 2005), remote sensing (e.g. Simis et al, 2005;Hunter et al, 2008aHunter et al, , 2008b, and in vitro extraction methods have all been used for cyanobacterial quantification (Sarada et al, 1999).…”

Ultraviolet Fluorescence LiDAR (UFL) as a Measurement Tool for Water Quality Parameters in Turbid Lake Conditions

“…Following these results, the extension of this analysis to the creation of a UFL-specific fluorescence emissions spectral library, including other divisions of phytoplankton (especially those with varying accessory pigments) and identification of end-member and mixture spectra is suggested, as has been done through laboratory and in vivo fluorescence spectroscopy elsewhere [20,35,36]. Quantitative analysis of other pigment concentrations, not possible during this study, would be an important component of such an exercise.…”

Emerging (Bio)Sensing Technology for Assessing and Monitoring Freshwater Contamination - Methods and Applications

“…They are presented in table 2 (Almeida et al, 2006;Allum et al, 2008;Dawan et al, 2011;Ding & Mutharasan, 2010;Gregora & Marsálek, 2005;Hu et al, 2008;Lindner et al, 2009;Long et al, 2009Long et al, , 2010Loypraseta et al, 2008;Pyo et al, 2005Pyo et al, , 2006Pyo & Jin, 2007;Queirós et al, 2011;Sheng et al, 2007;Xia et al, 2010Xia et al, , 2011Zhang et al, 2007). Optical immuno-based techniques are by large the most widely techniques that have been developed for the detection of MCs Hu et al, 2008;Long et al, 2010;Loypraseta et al, 2008;Pyo et al, 2005Pyo et al, , 2006Pyo & Jin, 2007;Sheng et al, 2007;Xia et al, 2010).…”