On-line liquid chromatography/tandem mass spectrometry simultaneous determination of opiates, cocainics and amphetamines in dried blood spots

Fast liquid chromatography/tandem mass spectrometry determination of cannabinoids in micro volume blood samples after dabsyl derivatization

“…water (containing 0.2% formic acid)/acetonitrile (containing 0.2% formic acid) (30/70, v/v), allowed the pre-concentration of the analytes onto the head of the column before the start of the gradient. An Atlantis C18 column was used in these studies and was the same as the one used for the quantitative analysis of other illicit drugs (opiates, amphetamines, cocainics) quantitative analysis in our laboratory [26,27]. Similar results were obtained with different octadecyl columns.…”

“…In the perspective of applying the developed method to simultaneous illicit drugs quantification in DUID cases, a part of deproteinized sample would be analyzed after dabsylation for cannabinoids, opiates, amphetamines, and the other underivatized part would be used for cocainics. In this context, the next step would be to apply the presented method to illicit drugs quantitative determination in dried blood spots [27].…”

“…Previously we have described a LC/MS-MS method coupled to on-line extraction allows a simultaneous quantification of opiates, cocainics and amphetamines using only 100 L of plasma or whole blood [26]. This on-line LC/MS-MS method has been successfully applied to dried blood spots using 30 L of whole blood [27]. This analytical technique illustrates perfectly the main objectives to reach for an acceptable LC/MS-MS method in forensic science i.e.…”

Current and future use of “dried blood spot” analyses in clinical chemistry

“…This detection method has been validated by the Food and Drug Administration (FDA) with sample stability ranging from 12 to over 90 days at room temperature [92]. In the field of toxicology, which is a major application of DBS [121], Saussereau et al [122] have, for example, developed a new drug screening method based on LC/MS using on-line extraction for the quantification of opiates, cocainics or amphetamines. The development of these new measurement techniques, based on LC/MS for xenobiotics, will greatly increase the interest of using DBS in clinical chemistry.…”

Fully automated drug screening of dried blood spots using online LC-MS/MS analysis

“…However, with modern mass spectrometry instruments, this issue is no longer a hurdle. The DBS technology emerged to further applications and markets such as therapeutic drug monitoring [3][4][5], pharmaco-toxicokinetic studies [6] and forensics [7][8][9][10][11][12][13]. Advantages of using DBS are simplified blood collection, reduced shipping, and storage costs and reduced analysis time and labor costs due to full automation [4,14].…”