Fast liquid chromatography/tandem mass spectrometry determination of cannabinoids in micro volume blood samples after dabsyl derivatization

Lacroix, Christian; Saussereau, Élodie

doi:10.1016/j.jchromb.2012.08.006

Cited by 50 publications

(33 citation statements)

References 46 publications

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“…Plasma THC content was quantified using fast liquid chromatography/mass spectrometry (LC/MS) adapted from (Irimia et al , 2015) and (Lacroix and Saussereau, 2012). 50 µL of plasma was mixed with 100 µL of deuterated internal standard (200 ng/mL THC-d3) and plasma cannabinoids were extracted into 400 µL ethyl acetate/acetonitrile (1:1) and then dried.…”

Section: Methodsmentioning

confidence: 99%

Inhaled delivery of Δ9-tetrahydrocannabinol (THC) to rats by e-cigarette vapor technology

et al. 2016

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Most human Δ9-tetrahydrocannabinol (THC) use is via inhalation, and yet few animal studies of inhalation exposure are available. Popularization of non-combusted methods for the inhalation of psychoactive drugs (Volcano®, e-cigarettes) further stimulates a need for rodent models of this route of administration. This study was designed to develop and validate a rodent chamber suitable for controlled exposure to vaporized THC in a propylene glycol vehicle, using an e-cigarette delivery system adapted to standard size, sealed rat housing chambers. The in vivo efficacy of inhaled THC was validated using radiotelemetry to assess body temperature and locomotor responses, a tail-flick assay for nociception and plasma analysis to verify exposure levels. Hypothermic responses to inhaled THC in male rats depended on the duration of exposure and the concentration of THC in the vehicle. The temperature nadir was reached after ~40 min of exposure, was of comparable magnitude (~3 °Celsius) to that produced by 20 mg/kg THC, i.p. and resolved within 3 hours (compared with a 6 hour time course following i.p. THC). Female rats were more sensitive to hypothermic effects of 30 min of lower-dose THC inhalation. Male rat tail-flick latency was increased by THC vapor inhalation; this effect was blocked by SR141716 pretreatment. The plasma THC concentration after 30 min of inhalation was similar to that produced by 10 mg/kg THC i.p.. This approach is flexible, robust and effective for use in laboratory rats and will be of increasing utility as users continue to adopt “vaping” for the administration of cannabis.

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Section: Methodsmentioning

confidence: 99%

Inhaled delivery of Δ9-tetrahydrocannabinol (THC) to rats by e-cigarette vapor technology

et al. 2016

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“…O coeficiente de determinação (r 2 ) foi 0,9915. Estudo com LC-MS-MS mostrou limite máximo do intervalo de linearidade até 250 ng mL -1 [4]. Com essa mesma técnica de análise, outro trabalho obteve o valor máximo de 500 ng mL -1 [22].…”

Section: Figura 5 Teste De Seletividade Cromatogramas De íOns Totaiunclassified

“…Os canabinóides mais abundantes são o THC, seu precursor canabidiol e o canabinol, um produto de decomposição formado espontaneamente a partir do THC [2] As principais matrizes para a análise de canabinóides em usuários são: cabelo [3], sangue [4] e urina [5]. Esta última é a amostra biológica mais utilizada para identificação do uso recente da maconha, por ser mais fácil de coletar, não invasiva e as concentrações e seus produtos de biotransformação são relativamente altas quando comparadas com as demais amostras biológicas [5].…”

Section: Introductionunclassified

Aplicação da microextração líquido-líquido dispersiva com solidificação da gota orgânica flutuante para determinação de 11-nor-9-carboxi-Δ9-tetraidrocanabinol em urina humana com derivação on-line no sistema de injeção cromatográfico

Dórea

Gomes²,

Prata³

et al. 2017

Scientia Plena

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Determinação de 11-nor-9-carboxi-Δ 9 -tetraidrocanabinol em urina humana por microextração líquido-líquido dispersiva com solidificação da gota orgânica flutuante e derivação online no sistema de injeção cromatográficoDetermination of 11-nor-9-carboxy-δ9-tetrahydrocannabinol in human urine by dispersive liquid-liquid microextraction with solidification of a floating organic drop and on-line derivatization in the injection system chromatographic H. A Cannabis sativa é a droga de abuso mais consumidas no mundo. Devido à baixa concentração dos metabólitos em amostras complexas, novas técnicas de extração estão sendo estudadas. O objetivo desse trabalho foi aplicar a Microextração Líquido-Líquido Dispersiva com Solidificação da Gota Orgânica Flutuante (DLLME-SFO) para determinar o 11-nor-9-carboxi-Δ 9 -tetraidrocanabinol (THC-COOH) em urina humana. Além disso, estabelecer um procedimento on line para a derivação do metabólito no injetor cromatográfico. O analito foi extraído da urina depois da hidrólise alcalina do glicuronídeo, usando o solvente de extração 1-dodecanol (30 µL) e o metanol (200 µL) como dispersor. NaCl 1% foi adicionado e o tempo de extração foi de 5 min. A melhor proporção entre BSTFA:extrato para derivação on line do THC-COOH foi 2:2, injetor operado no modo split 1:20 e temperatura de 240 °C. O extrato foi analisado por cromatografia gasosa acoplada a espectrometria de massas no modo tandem (GC-MS/MS), depois da definição dos ions pais (m/z), da energia de excitação (modo ressonante) e dos ions filhos para a quantificação e confirmação (m/z) do canabinóide e do padrão interno deuterado. Os limites de detecção (LD) e de quantificação (LQ) foram 1 e 10 ng mL -1 , respectivamente. O método analítico proposto apresentou vantagens com relação à sensibilidade, especificidade, linearidade e custo com relação à extração em fase sólida, assim como é adequado à química verde e seus limites são apropriados para analisar THC-COOH em urina humana.Palavras-chave: THC-COOH, urina, DLLME-SFO.Cannabis sativa is the most used drug in the world. Due to the low concentration of metabolites in complex samples, new extraction techniques are being studied. The objective of this study was to develop an analytical method by applying the technique of Dispersive Liquid-Liquid MicroextractionSolidification of a Floating Organic Drop (DLLME-SFO) to determine 11-nor -9-carboxyl -Δ 9 -tetrahydrocannabinol (THC-COOH) in human urine sample. In addition, it was established an online procedure for derivatisation of the metabolite in the chromatographic injector. After of the glucuronide alkaline hydrolysis, the analyte was extracted with 1-dodecanol (30 µL) and methanol (200 µL), as extraction and dispersive solvents, respectively, addition of 1 % NaCl and 5 min to extraction time. The best proportion between BSTFA:extract for on line derivatization of the THC-COOH was 2:2, the split was 1:20 and 240 °C for temperature. The extract was analyzed by gas chromatography coupled to tandem mass spectrometry (GC-MS/MS), after definition ...

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“…Generally, the ionization efficiency of CBs is extremely low due to their non-polar nature, resulting in poor LODs. To solve this problem, Lacroix and Saussereau functionalized the phenolic groups of CBs with chloride dabsyl to form a product with a tertiary amine and then significantly improved the LODs of cannabinoids [66]. In this approach, LC-ESI-MS/MS technique was used for the quantitative determination of THC, 11-OH–THC, THC–COOH, CBN and CBD in microvolume blood samples.…”

Section: Determination Of Neurochemicals By Lc-ms Related Techniquesmentioning

confidence: 99%

“…The LC-MS approaches also showed great potentiality to separate and detect the neurological drugs in biological samples with high sensitivity and high speed. Although the LC-MS techniques are effective for neurodrug analysis, they require specially designed columns, derivatization steps, and lengthy procedures for extraction and preconcentration of neurodrugs in biological samples [27, 44, 49, 63, 66]. …”

Section: Determination Of Neurochemicals By Lc-ms Related Techniquesmentioning

confidence: 99%

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Kailasa

2013

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Recently, mass spectrometric related techniques have been widely applied for the identification and quantification of neurochemicals and their metabolites in biofluids. This article presents an overview of mass spectrometric techniques applied in the detection of neurological substances and their metabolites from biological samples. In addition, the advances of chromatographic methods (LC, GC and CE) coupled with mass spectrometric techniques for analysis of neurochemicals in pharmaceutical and biological samples are also discussed.

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Fast liquid chromatography/tandem mass spectrometry determination of cannabinoids in micro volume blood samples after dabsyl derivatization

Cited by 50 publications

References 46 publications

Inhaled delivery of Δ9-tetrahydrocannabinol (THC) to rats by e-cigarette vapor technology

Inhaled delivery of Δ9-tetrahydrocannabinol (THC) to rats by e-cigarette vapor technology

Aplicação da microextração líquido-líquido dispersiva com solidificação da gota orgânica flutuante para determinação de 11-nor-9-carboxi-Δ9-tetraidrocanabinol em urina humana com derivação on-line no sistema de injeção cromatográfico

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

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