On-line isotachophoretic sample focusing for loadability enhancement in capillary electrochromatography–mass spectrometry

Mazereeuw, M.; Spikmans, Val; Tjaden, U.R.; Greef, J. van der

doi:10.1016/s0021-9673(00)00259-4

Cited by 31 publications

(16 citation statements)

References 34 publications

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“…Besides this, the on-line isotachophoretic stage can serve also for a loadability enhancement (due to a large inner diameter of the ITP capillary). Both of these effects are then responsible for a dramatic reduction of the sample concentration detection limits through simultaneous acting of (i) large volume injection and (ii) analyte stacking (Mazereeuw et al, 2000). In the ITP-CEC combination (Fig.…”

Section: Itp-cecmentioning

confidence: 99%

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Column Coupling Electrophoresis in Biomedical Analysis

Mikuš¹,

Maráková²

2011

Biomedical Engineering - From Theory to Applications

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Section: Itp-cecmentioning

confidence: 99%

“…The ITP-CEC-MS hyphenation seems to be one of the most promissing methods for the fully automatized biomedical chiral analyses such as enantioselective pharmacokinetic studies, metabolomics, etc. (Mazereeuw et al, 2000). (1 and 2) and 75 m (3) are used.…”

Section: Itp-cecmentioning

confidence: 99%

Column Coupling Electrophoresis in Biomedical Analysis

Mikuš¹,

Maráková²

2011

Biomedical Engineering - From Theory to Applications

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“…Mazereeuw et al [35] have used on-line isotachophoretic sample focusing for loadability enhancement in CEC-MS. A coupled-column setup was used with a 220 mm ID capillary, in which counterflow focusing was performed, connected via a T-junction to a 75 mm ID CEC capillary. The use of isotachophoretic focusing resulted in a dramatic reduction of the analyte LODs.…”

Section: Clenbuterol and Salbutamolmentioning

confidence: 99%

Recent applications of capillary electrophoresis‐electrospray ionisation‐mass spectrometry in drug analysis

Smyth¹

2005

Electrophoresis

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A critical review of applications for the period 2000-2004, taken from the Web of Knowledge database, of the technique capillary electrophoresis-electrospray ionisation-mass spectrometry (CE-ESI-MS) in drug analysis is presented. The review is concerned with molecules of mass less than 500 Da, chosen according to selected structural classes in which they give ESI signals primarily as [M+H](+) ions although other ions, such as [M-H](-), [M+Na](+), and [M+NH(4)](+), are also reported. These structural classes are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, 1,4-benzodiazepines, other heterocyclic hypnotics, carbohydrates, sulphonylureas, anthracyclines, sulphonamides, penicillins, cephalosporins, tetracyclines, nitrocatechols, steroids, flavonoids/polyphenols, cannabinols, and miscellaneous molecules. Details are given on the fragmentations, where available, that these ionic species exhibit in-source and in ion-trap, triple quadrupole, and time of flight-mass spectrometers. The review gives a critical evaluation of these recent CE-ESI-MS analytical methods in drug analysis. Analytical information on, for example, sample concentration techniques, CE separation conditions, recoveries from biological media and limits of detection (LODs) are provided. Potential applications of CE-MS to particular drugs or drug classes are also briefly discussed in the text.

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“…Such coupling allows flexible and precise manipulation of both extraction and separation processes. Tjaden and co-workers [22] reported the on-line preconcentration of charged solutes via isotachophoretic sampling focusing in untreated fused-silica capillaries prior to CEC separation and UV/MS detection. Jinno and co-workers [23] established the on-line coupling of fiber-in-tube solid-phase microextraction (SPME) with CEC and reported its successful application to the preconcentration and separation of phthalate mixtures in water samples.…”

Section: Introductionmentioning

confidence: 99%

Development of in‐tube solid‐phase microextraction coupled to pressure‐assisted CEC and its application to the analysis of propranolol enantiomers in human urine

Lin

Zheng

et al. 2007

Electrophoresis

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A successful hyphenation of in-tube solid-phase microextraction (SPME) and pressure-assisted CEC (pCEC) was developed by installing a poly(methacrylic acid-co-ethylene glycol dimethacrylate) monolithic capillary to the six-port valve in a CEC system. The device designed was appropriate for on-line in-tube SPME coupled to pCEC or muHPLC. The evaluation of this hyphenation was first carried out for in-tube SPME-muHPLC with analytical capillaries packed with 3 microm octadecyl silica (ODS). Theobromine (TB), theophylline (TP), and caffeine (CA) were chosen as model drugs for an easy comparison with the results obtained by in tube SPME-HPLC. The detection limits of these three analytes were improved more than 100 times when compared with the direct analysis by muHPLC. Then in-tube SPME-pCEC with CEC capillaries packed with perphenylcarbamoylated beta-CD-bonded silica particles was applied to the determination and analysis of propranolol enantiomers in human urine. Under optimal extraction and separation conditions, the experimental LODs were 4 and 7 ng/mL for (S)-propranolol and (R)-propranolol, respectively. The calibration curves showed good linearity for both (S)-propranolol (R(2) = 0.9997) and (R)-propranolol (R(2) = 0.9996) over the concentration range from 20 to 5000 ng/mL. Reproducibility of the method was also investigated with intra- and interday precisions lower than 10% for both enantiomers at different concentration levels.

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On-line isotachophoretic sample focusing for loadability enhancement in capillary electrochromatography–mass spectrometry

Cited by 31 publications

References 34 publications

Column Coupling Electrophoresis in Biomedical Analysis

Column Coupling Electrophoresis in Biomedical Analysis

Recent applications of capillary electrophoresis‐electrospray ionisation‐mass spectrometry in drug analysis

Development of in‐tube solid‐phase microextraction coupled to pressure‐assisted CEC and its application to the analysis of propranolol enantiomers in human urine

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