On-line determination of glucose in biotechnological processes: comparison between FIA and an in situ enzyme electrode

Filippini, Claudio; Sonnleitner, Bernhard; Fiechter, Armin; Bradley, J.; Schmid, Rolf D.

doi:10.1016/0168-1656(91)90243-o

Cited by 43 publications

(10 citation statements)

References 8 publications

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“…The solutions were kept dark all time and did not change in concentration to a measurable extent. H 2 O 2 was quanti®ed with ABTS according to [6].…”

Section: Methodsmentioning

confidence: 99%

Robust oxygen supply by controlled addition of hydrogen peroxide to microbial cultures

Sonnleitner

Hahnemann

1997

Bioprocess Engineering

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The supply of oxygen can be improved by the direct addition of hydrogen peroxide to cultures of aerobic microbes expressing suf®cient amounts of catalase. This is of special interest if normal aeration has to be kept low, for instance, in order to minimize evaporation of volatile compounds (either substrates or products) or to minimize foaming. Also, if the mechanical power input to the bioreactor is or has to be limited, addition of hydrogen peroxide may be useful.The appropriate dosage of hydrogen peroxide can be simply determined by a controller of the oxygen partial pressure or of the oxygen content in the exhaust gas using various control algorithms. The added hydrogen peroxide can be either a stabilized concentrate, e.g. 30%, or any dilute form of this. In high density cultures, Pseudomonas cells tolerated even harsh controller disturbances. This approach proved to be very robust and reliable.

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“…The solutions were kept dark all time and did not change in concentration to a measurable extent. H 2 O 2 was quanti®ed with ABTS according to [6].…”

Section: Methodsmentioning

confidence: 99%

Robust oxygen supply by controlled addition of hydrogen peroxide to microbial cultures

Sonnleitner

Hahnemann

1997

Bioprocess Engineering

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“…Fiber optic-based sensors have been used in situ for NADH measurements in bioreactors (Luong and Mulchandani, 1990). In situ electrode probes for monitoring glucose in Escherichia coli and Saccharomyces cerevisiae fermentations have also been reported Enfors, 1983, 1984;Bradley et al, 1989a, b, c;Filippini et al, 1991) and are described subsequently. Furthermore, in order to maximize the response, conditions of operation for these detectors need to be optimized with respect to temperature, pH, and ionic strength.…”

Section: In Situ Monitoringmentioning

confidence: 95%

Principles and Applications of Biosensors for Bioprocess Monitoring and Control

Mulchandani

Bassi

1995

Critical Reviews in Biotechnology

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Biosensors are useful analytical devices that can be integrated with on-line process monitoring schemes. In this article, the principles and applications of these devices for bioprocess monitoring are considered. Several different types of biosensors are described, and the applications and limitations of flow injection analysis (FIA) for these applications are discussed. It is hoped that the background provided here can be useful to researchers in this area.

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“…Besides a few sensors based on enzymatic analysis of medium compo nents, e.g. for glucose analysis Filippini et al, 1991], all these sensors belong to the group of optical sensors, which includes: 1) sensors for measurement of culture fluorescence; 2) sensors for measurement of light scattering; and 3) sensors based on near infrared spectroscopy. Measurements of light scattering is useful for on-line monitoring of the biomass concentration, as illustrated for cultivation with S. cerevisiae [Locher, 1991;Lid6n, 1993a].…”

Section: In-situ Sensorsmentioning

confidence: 99%

Physiological Engineering

1997

Physiological Engineering Aspects of Penicillium Chrysogenum

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On-line determination of glucose in biotechnological processes: comparison between FIA and an in situ enzyme electrode

Cited by 43 publications

References 8 publications

Robust oxygen supply by controlled addition of hydrogen peroxide to microbial cultures

Robust oxygen supply by controlled addition of hydrogen peroxide to microbial cultures

Principles and Applications of Biosensors for Bioprocess Monitoring and Control

Physiological Engineering

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