On-line capillary gas chromatography with automated liquid sampling, a powerful tool in biotechnology

Filippini, Claudio; Moser, Johann; Sonnleitner, Bernhard; Fiechter, Armin

doi:10.1016/0003-2670(91)85091-6

Cited by 35 publications

(17 citation statements)

References 17 publications

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“…Gas chromatography was chosen for the measurement of ethanol, acetic acid, and acetaldehyde based on the method presented by Filippini et al (1991). The gas chromatograph GC-14A (Shimadzu, Tokyo, Japan) was modified with a heated piston injector (Mess und Apparatetechnik, Garching, Germany), wich allows injecting a well-defined volume of 1-L liquid sample into the carrier steam (N 2 ) with instantaneous evaporation, to enter a capillary column (Permabond FFAP, Macherey & Nagel, Oensingen, Switzerland).…”

Section: Bioprocess Set-upmentioning

confidence: 99%

On‐line stoichiometry and identification of metabolic state under dynamic process conditions

Herwig

Marison

Stockar

2001

Biotech & Bioengineering

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A method for the on-line calculation of conversion rates and yield coefficients under dynamic process conditions was developed. The method is based on cumulated mass balances using a moving average method. Elemental balances were used to test the measured cumulated quantities for gross errors and inappropriate stoichiometry definition followed by data reconciliation and estimation of non-measured conversion rates, using a bioprocess set-up including multiple on-line analysis techniques. The quantitative potential of the proposed method is demonstrated by executing transient experiments in aerobic cultures of Saccharomyces cerevisiae on glucose. Rates and yield coefficients could be consistently quantified in shift-up, shift-down, and accelerostat experiments. The method shows the capability to describe quantitatively transient changes in metabolism including uncoupling of catabolism and anabolism, also for the case when multiple components of metabolism are not measured. The validity of the experiment can be evaluated on-line. Additionally, the method detects with high sensitivity inappropriate stoichiometry definition, such as a change in state of metabolism. It was shown that concentration values can be misleading for the identification of the metabolic state. In contrast, the proposed method provides a clear picture of the metabolic state and new physiological regulations could be revealed. Hence, the novelty of the proposed method is the on-line availability of consistent stoichiometric coefficients allowing a significant speed up in strain characterization and bioprocess development using minimal knowledge of the metabolism. Additionally, it opens up the use of transient experiments for physiological studies.

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Section: Bioprocess Set-upmentioning

confidence: 99%

On‐line stoichiometry and identification of metabolic state under dynamic process conditions

Herwig

Marison

Stockar

2001

Biotech & Bioengineering

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“…In practice, the use of filtration membranes with an MWCO of 50-100 kDa is suitable for most applications [78]. Most on-line filtration applications are used in combination with LC [88,89,90], although some GC applications have also been published [91,92].…”

Section: Filtrationmentioning

confidence: 99%

“…Membranes can be utilised in sample preparation in several ways [75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115]. Very generally, a membrane can be classified as a selective barrier between two phases [75,76,77].…”

Section: Membrane-based Sample Pretreatmentmentioning

confidence: 99%

Approaches for on-line coupling of extraction and chromatography

Hyötyläinen

Riekkola

2004

Analytical and Bioanalytical Chemistry

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This review provides an overview of the approaches available in order to perform on-line coupling of various extraction techniques with liquid and gas chromatography, for the analysis of semivolatile and nonvolatile analytes in liquid and solid samples. The main focus is on the instrumental set-up of these techniques. Selected real applications are described by way of illustration. The extraction methods suitable for on-line coupling covered in this review are: liquid-liquid extraction, solid-phase extraction, membrane-based techniques, pressurised liquid extraction, supercritical fluid extraction, and microwave- and sonication-assisted extractions. The following systems are not covered in this review: on-line coupled solid-phase extraction-liquid chromatography, purge-and-trap-GC, and membrane extraction with a sorbent interface-GC.

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“…So ist z. B. die Radiofrequenzspektroskopie die weitest verbreitete Methode für die Biomassebestimmung [18] [27,28] und der Prozess gesteuert werden [29]. [12].…”

Section: Bioprozess-entwicklungunclassified

Prozess Analytische Technologie in der Biotechnologie

Herwig¹

2010

Chemie Ingenieur Technik

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On-line capillary gas chromatography with automated liquid sampling, a powerful tool in biotechnology

Cited by 35 publications

References 17 publications

On‐line stoichiometry and identification of metabolic state under dynamic process conditions

On‐line stoichiometry and identification of metabolic state under dynamic process conditions

Approaches for on-line coupling of extraction and chromatography

Prozess Analytische Technologie in der Biotechnologie

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