The Korea Expressway Corporation has studied various preventive measures to prevent drowsiness driving accidents, such as providing various drowsiness driven prevention measures, establishing drowsiness rest areas, and operating alarms on drowsiness driving in highway tunnel have been implemented. Recently, the Korea Transportation Safety has also used the monitoring of the driven state (DSM-Driven State Monitoring) and the driving information of the vehicle (VDI-Vehicle Driving Information) to confirm the state of the driver in the running car and perform a drowsiness driving and We are showing good reaction by introducing a drowsiness driven prevention system which sounds vibration and warning sound. As described above, when a driver wakes up during a drowsiness operation, a warning sound plays an important role by installing it in a tunnel or in a car. In this paper, we further developed the warning sound for battle against drowsiness used in the tunnel of the existing highway and studied focusing on using inside the vehicle. This research aims to verify and further improve the warning sound part of the in-vehicle drowsiness driven prevention system which is just introduced and enforced, effectively and contribute effectively to prevent drowsiness driven accidents.