A method to determine the characteristic impedance of transmission lines considering the substrate loss is presented. The extracted data shows that at microwave frequencies the finite loss tangent parameter impacts the characteristic impedance even in a low-loss substrate.Introduction: Thru -reflect -line (TRL) [1] is the most popular calibration method applied when measuring using transmission line (TL) test fixtures. However, the accuracy of TRL depends on the reliable determination of the characteristic impedance (Z c ) of the TL that establishes the reference for the calibrated data [2]. For years, the extremely simple method in [3] has been used to obtain Z c in semiconductor [4] as well as in printed circuit board (PCB) technologies [5]. Unfortunately, this method neglects the effect of the per unit length conductance (G) of the TL by assuming that the leakage currents and the effective loss tangent (tand eff ) of the dielectric environment surrounding the line are very small. Whereas the impact of the leakage currents on PCB TLs is not significant, the effect of tand eff becomes accentuated as the operation frequency ( f ) increases, even in low-loss substrates. For this reason, alternative approaches have been proposed for obtaining Z c [2, 6]. These methods, however, leave unphysical fluctuations in the determined data, especially for relatively long lines such as those used on PCB technology [6]. This problem is solved in this Letter by directly determining Z c from the experimental complex propagation constant (g) considering the impact of the finite tand eff parameter.