On-chip rapid drug screening of leukemia cells by acoustic streaming

Abstract

Rapid and personalized single-cell drug screening testing plays an essential role in acute myeloid leukemia drug combination chemotherapy. Conventional chemotherapeutic drug screening is a time-consuming process because of the natural...

An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening

“…In terms of single-cell cloning, the house-like microchamber array is ideal for massive single-cell capture and culture to allow efficient single-cell derived clone formation. Although some reported microfluidic devices have achieved great single-cell capture efficiency, the on-site cell maintenance capability for high-throughput cell cloning is still technically challenging [ 22 , 23 ]. Furthermore, the integration of an on-chip CGG greatly promotes our device as a simple but efficient tool for cancer drug effect analysis at the single cell level [ 13 , 22 , 41 ].…”

“…With regard to this, Pei et al [ 22 ] combined a concentration gradient generator (CGG) with single cell array to explore the bioeffects of an anticancer drug on single circulating tumor cells collected from cancer patient samples. By integrating high frequency acoustic waves in a concentration gradient microfluidic device, Zhao et al [ 23 ] developed a single-cell drug screening acceleration method for the evaluation of AML chemotherapy. While these studies illustrate the feasibility of on-chip CGG to facilitate drug-screening studies, these proposed platforms could only handle several hundreds of single cells and lacked the capability for long-term cell retention and on-chip cell culture.…”

Microfluidic nanodevices for drug sensing and screening applications

“…In this regard, microfluidic technology has emerged as a promising platform for miniaturized and controlled drug screening, even at the cellular level ( Ramezankhani et al, 2022 ). Compared to single-drug analysis in conventional petri dish or animal model methods, microfluidic platforms can simultaneously provide high-precision multidrug ( Jiang et al, 2003 ; Mitxelena-Iribarren et al, 2019 ; Zhao et al, 2021 ) and multiconcentration ( Humphries et al, 2018 ; Bavli et al, 2016 ; W. bin J. B. Lee et al, 2019 ; W. Liu et al, 2019 , Liu et al, 2019 ) screening with high sensitivity and faster response times.…”

Microfluidic-based in vitro thrombosis model for studying microplastics toxicity

“…16–25 However, there is still a gap in the study of the effects of MPs on the highly interacting vascular system. Optofluidic techniques 2,26–30 enable precise optical manipulation of cells, particles 31–39 and reconfigurable tissue functionalization, 40–43 and so can be exploited to study the interaction of MPs with blood vessels.…”