On-chip impedimetric detection of bacteriophages in dairy samples

García-Aljaro, Cristina; Muñoz-Berbel, Xavier; Muñoz, Francisco

doi:10.1016/j.bios.2008.08.047

Cited by 26 publications

(18 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A surface plasmon resonance assay detected as low as 10 2 coliphages/ml of wastewater and their replication could be followed in real time. The same research group later proposed a phage detection assay using impedimetry [ 50 ]. The authors demonstrated that it was possible to detect phages using the current variation generated by biofilm degradation in a dairy sample [ 50 ].…”

Section: Methods Of Detectionmentioning

confidence: 99%

“…The same research group later proposed a phage detection assay using impedimetry [ 50 ]. The authors demonstrated that it was possible to detect phages using the current variation generated by biofilm degradation in a dairy sample [ 50 ]. Still, this latest method had several limitations: i) the host bacteria has to form a biofilm on a chip, ii) only phages specific to the bacteria could be detected, iii) the technique could only assess the presence or absence of the phage and thus was not quantitative.…”

Section: Methods Of Detectionmentioning

confidence: 99%

See 1 more Smart Citation

Bacteriophages of lactic acid bacteria and their impact on milk fermentations

2011

View full text Add to dashboard Cite

Every biotechnology process that relies on the use of bacteria to make a product or to overproduce a molecule may, at some time, struggle with the presence of virulent phages. For example, phages are the primary cause of fermentation failure in the milk transformation industry. This review focuses on the recent scientific advances in the field of lactic acid bacteria phage research. Three specific topics, namely, the sources of contamination, the detection methods and the control procedures will be discussed.

show abstract

Section: Methods Of Detectionmentioning

confidence: 99%

Section: Methods Of Detectionmentioning

confidence: 99%

Bacteriophages of lactic acid bacteria and their impact on milk fermentations

2011

View full text Add to dashboard Cite

show abstract

“…In a recent study, García-Aljaro et al 88 developed a rapid phage detection method based on the evaluation of impedance changes during infection of a host-biofilm established onto metal (platinum and gold) microelectrodes. The infection and subsequent host cell lysis was monitored by non-faradaic impedance spectroscopy in milk samples.…”

Section: Detection and Quantification Of Dairy Bacteriophages: A Briementioning

confidence: 99%

Bacteriophages and dairy fermentations

2012

View full text Add to dashboard Cite

This review highlights the main strategies available to control phage infection during large-scale milk fermentation by lactic acid bacteria. The topics that are emphasized include the factors influencing bacterial activities, the sources of phage contamination, the methods available to detect and quantify phages, as well as practical solutions to limit phage dispersion through an adapted factory design, the control of air flow, the use of adequate sanitizers, the restricted used of recycled products, and the selection and growth of bacterial cultures.

show abstract

“…In addition, the impedance spectroscopy approach is limited by the lack of diversity of antibodies and the efficiency of absorbing microbes on a sensing platform. 25 …”

Section: Resultsmentioning

confidence: 99%

Capture and Detection of T7 Bacteriophages on a Nanostructured Interface

Han

Wang

Das

et al. 2014

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

A highly ordered array of T7 bacteriophages was created by the electrophoretic capture of phages onto a nanostructured array with wells that accommodated the phages. Electrophoresis of bacteriophages was achieved by applying a positive potential on an indium tin oxide electrode at the bottom of the nanowells. Nanoscale arrays of phages with different surface densities were obtained by changing the electric field applied to the bottom of the nanowells. The applied voltage was shown to be the critical factor in generating a well-ordered phage array. The number of wells occupied by a phage, and hence the concentration of phages in a sample solution, could be quantified by using a DNA intercalating dye that rapidly stains the T7 phage. The fluorescence signal was enhanced by the intrinsic photonic effect made available by the geometry of the platform. It was shown that the quantification of phages on the array was 6 orders of magnitude better than could be obtained with a fluorescent plate reader. The device opens up the possibility that phages can be detected directly without enrichment or culturing, and by detecting phages that specifically infect bacteria of interest, rapid pathogen detection becomes possible.

show abstract

On-chip impedimetric detection of bacteriophages in dairy samples

Cited by 26 publications

References 14 publications

Bacteriophages of lactic acid bacteria and their impact on milk fermentations

Bacteriophages of lactic acid bacteria and their impact on milk fermentations

Bacteriophages and dairy fermentations

Capture and Detection of T7 Bacteriophages on a Nanostructured Interface

Contact Info

Product

Resources

About