On-chip electroporation and impedance spectroscopy of single-cells

Bürgel, Sebastian C.; Escobedo, Carlos; Haandbæk, Niels; Hierlemann, Andreas

doi:10.1016/j.snb.2014.12.016

Cited by 74 publications

(49 citation statements)

References 48 publications

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“…Dielectric spectroscopy (DS) is a noninvasive, label‐free, fast, and promising technique for measuring type, size, and dielectric properties of biological cells in real time . This technique involves application of a small test voltage and measurement of AC current in a wide range of frequencies; providing an impedance spectrum . Biophysical models, such as the single‐shell model, can be used to analyze the measured impedance spectrum.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

et al. 2017

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Dielectric spectroscopy (DS) is a non-invasive, label-free, fast, and promising technique for measuring dielectric properties of biological cells in real time. We demonstrate a microchip that consists of electro-activated micro-well arrays for positive dielectrophoresis (pDEP) assisted cell capture, DS measurements, and negative dielectrophoresis (nDEP) driven cell unloading; thus, providing a high throughput cell analysis platform. To the best of our knowledge, this is the first microfluidic chip that combines electro-activated micro-wells and DS to analyze biological cells. Device performance is tested using Saccharomyces Cerevisiae (yeast) cells. DEP response of yeast cells is determined by measuring their Clausius-Mossotti (CM) factor using biophysical models in parallel plate micro-electrode geometry. This information is used to determine the excitation frequency to load and unload wells. Effect of yeast cells on the measured impedance spectrum was examined both experimentally and numerically. Good match between the numerical and experimental results establishes the potential use of the micro-chip device for extracting sub-cellular properties of biological cells in a rapid and non-expensive manner.

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Section: Introductionmentioning

confidence: 99%

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

et al. 2017

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“…A variety of methods have been developed that perform localized electroporation ( Figure 2B). One way this can be achieved is by incorporating a microchip containing electrodes inside a microfluidic channel, in which the cells flow through the channel whilst simultaneously being subjected to localized electroporation [63,64]. This method has been shown to enable fluorescent dyes and siRNAs to be delivered into single cells as they pass through the electric field [63,64].…”

Section: Bulk and Localized Electroporationmentioning

confidence: 99%

Methods for protein delivery into cells: from current approaches to future perspectives

Chau

Actis

Hewitt

2020

Biochemical Society Transactions

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The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into defined complexes and subject to biophysical analyses prior to their delivery into cells. Here, we review new approaches to the injection and electroporation of proteins into cultured cells. In particular, we focus on how recent developments in nanoscale injection probes and localized electroporation devices enable proteins to be delivered whilst minimizing cellular damage. Moreover, we discuss how nanopore sensing may ultimately enable the quantification of protein delivery at single-molecule resolution.

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“…7, an equivalent circuit was proposed based on the lEoN structure on the condition that the lEoN was immersed in the bio-tissues. [28][29][30][31][32][33][34] The total sensor output is a combination of the resistance (R) and capacitance (C) of parylene C and tissue samples, double layer capacitance, and constant phase element (CPE).…”

Section: -6mentioning

confidence: 99%

Micro electrical impedance spectroscopy on a needle for ex vivo discrimination between human normal and cancer renal tissues

et al. 2016

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The ex-vivo discrimination between human normal and cancer renal tissues was confirmed using lEoN (micro electrical impedance spectroscopy-on-a-needle) by measuring and comparing the electrical impedances in the frequency domain. To quantify the extent of discrimination between dissimilar tissues and to determine the optimal frequency at which the discrimination capability is at a maximum, discrimination index (DI) was employed for both magnitude and phase. The highest values of DI for the magnitude and phase were 5.15 at 1 MHz and 3.57 at 1 kHz, respectively. The mean magnitude and phase measured at the optimal frequency for normal tissues were 5013.40 6 94.39 X and À68.54 6 0.72 , respectively; those for cancer tissues were 4165.19 6 70.32 X and À64.10 6 0.52 , respectively. A statistically significant difference (p< 0.05) between the two tissues was observed at all the investigated frequencies. To extract the electrical properties (resistance and capacitance) of these bio-tissues through curve fitting with experimental results, an equivalent circuit was proposed based on the lEoN structure on the condition that the lEoN was immersed in the bio-tissues. The average and standard deviation of the extracted resistance and capacitance for the normal tissues were 6.22 6 0.24 kX and 280.21 6 32.25 pF, respectively, and those for the cancer tissues were 5.45 6 0.22 kX and 376.32 6 34.14 pF, respectively. The electrical impedance was higher in the normal tissues compared with the cancer tissues. The lEoN could clearly discriminate between normal and cancer tissues by comparing the results at the optimal frequency (magnitude and phase) and those of the curve fitting (extracted resistance and capacitance). Published by AIP Publishing.

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On-chip electroporation and impedance spectroscopy of single-cells

Cited by 74 publications

References 48 publications

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

Dielectrophoresis assisted loading and unloading of microwells for impedance spectroscopy

Methods for protein delivery into cells: from current approaches to future perspectives

Micro electrical impedance spectroscopy on a needle for ex vivo discrimination between human normal and cancer renal tissues

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