On carbohydrate metabolism of cultured carrot root explants

Bender, L.; Pauler, B.; Neumann, K.–H.

doi:10.1007/bf00043150

Cited by 7 publications

(6 citation statements)

References 8 publications

(10 reference statements)

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“…Our results on label incorporation are in agreement with the work of Thorpe and Beaudoin-Eagan (1984) in tobacco callus cultures fed with lac-glucose where most of the label was recovered in the sugar fraction, but amino acids and organic acids were also moderately labelled. Similar observations were made by Bender et al, (1987) in their study on the carbohydrate metabolism of cultured carrot root explants. Label applied as 14C-glucose or 14C-fructose was mainly recovered as sucrose in the explants.…”

Section: Metabolism Of the Absorbed Sugarssupporting

confidence: 77%

“…This type of uptake mechanism has been identified for both the uptake of hexoses and sucrose (Lozada and Cardemil, 1990;de Klerk-Kiebert et al, 1983). Subsequent to uptake, glucose and fructose are apparently phosphorylated in the cytosol (Kanabus et al, 1986) and converted into each other (Bender et al, 1987). These phosphorylated sugars are then channelled into different metabolic pathways.…”

Section: Introductionmentioning

confidence: 99%

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Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Riek

Piqueras

Debergh

1997

Plant Cell Tiss Organ Cult

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Uptake and metabolism of sucrose in micropropagated Rosa multiflora using the double layer technique was investigated. In the multiplication as well as the root induction stage, hydrolysis of sucrose in the culture medium was observed. A mathematical model was developed to quantify sucrose hydrolysis and the uptake of sucrose, glucose and fructose, based on the time series for the different sugars in the culture medium. These data were linked to a study of the sugar metabolism in the microshoots. After 48 h of incubation on 14C-[U]-glucose containing medium, the incorporated label was mainly detected in the ethanol soluble fraction; within this fraction sucrose was the most important compound. This indicates a significant re-synthesis of sucrose in the plant material after the uptake of hexose. To assess the extent that different enzymes of sucrose metabolism (invertases, sucrose synthase and sucrose-P-synthase) were involved, their activity in different plant parts (of final stage III microshoots) were assayed. A decreasing gradient for sucrose metabolising enzymes from the roots toward the leaves gave a good indication of how the different tissues depend on sucrose absorbed from the medium.

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Section: Metabolism Of the Absorbed Sugarssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Riek

Piqueras

Debergh

1997

Plant Cell Tiss Organ Cult

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“…If a similar mechanism is operative in carrot cells this would explain the observed rapid conversion of fructose into glucose and sucrose, the latter compound most probably being stored in the vacuole. Employing carrot root callus cultures supplied with 14C labeled sugars, Bender et al (2) arrived at a similar conclusion concerning the fate of exogeneously supplied sucrose, glucose, and fructose. In that study it was also observed that only a limited amount of the supplied hexoses were immediately catabolized, whereas over 95% were resynthesized into sucrose intracellularly, depending on the growth rate of the tissue under investigation.…”

Section: Discussionmentioning

confidence: 77%

“…Numerous observations on plant cell suspension cultures suggest that before entry into the cell, sucrose is first hydrolyzed into its constituent hexoses, glucose, and fructose (2,10,11), by a cell wall-associated invertase (18,21,32). However, total hydrolysis of sucrose does not seem to be necessary for the in vitro growth of plant cells (11), and the degree of hydrolysis varies between plant species (6,15,20,32).…”

mentioning

confidence: 98%

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR

Dijkema¹,

Vries²,

Booij³

et al. 1988

Plant Physiol.

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The uptake and utilization of sucrose by embryogenic suspension cultures of carrot (Daucus carota L.) growing in the presence of 2,4-D and by somatic embryos derived from these cultures was monitored using (13)C nuclear magnetic resonance. The exogeneously supplied sucrose was completely hydrolyzed before cell entry; glucose was taken up preferentially when the cells were cultured in the presence of 2,4-D, while glucose and fructose were utilized at similar rates by somatic embryos in the absence of 2,4-D. Both suspension cells and somatic embryos accumulated high intracellular levels predominantly of glucose and sucrose, the latter being resynthesized intracellularly from the constitutive hexoses. Initially, fructose was converted mainly into glucose and sucrose rather than being catabolized directly through glycolysis or the pentose phosphate pathway. Carbohydrate supply that exceeded cellular demand resulted in intracellular accumulation of mono- or disaccharides. The capacity of cultured carrot cells to produce somatic embryos appeared to be positively correlated with high intracellular levels of glucose.

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“…These findings suggest that the accumulation of F in the culture medium of R. jasminoides is not linked to an inability to utilize this monosaccharide, but rather to the fact that G is a better substrate for cell respiration, as reported by Sagishima et al (1989) for cell suspension cultures of Catharanthus roseus. The preferential uptake of G has also been described for cell suspension cultures of carrot (Kanabus et al, 1986;Bender et al, 1987), soybean (Spilatro and Anderson, 1988), birch (Nuutila and Kauppinen, 1992), and bean (Botha and O_Kennedy, 1998) and has been attributed to a higher affinity of the sugar transport proteins for this monosaccharide when compared to F (Tubbe and Buckhout, 1992;Sherson et al, 2003). Therefore, the behavior of cell suspension cultures of R. jasminoides regarding sugar utilization seems to be typical for undifferentiated cell suspension cultures of dicotyledons.…”

Section: Resultsmentioning

confidence: 82%

Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

Kretzschmar

Júnior

Braga

2007

In Vitro Cell.Dev.Biol.-Plant

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The primary utilization of carbohydrates by cell suspension cultures of Rudgea jasminoides, a native woody Rubiaceae from tropical forests, was investigated. Sucrose, glucose + fructose, glucose, or fructose were supplied as carbon sources. The growth curves of R. jasminoides cultured in glucose + fructose, glucose, or fructose showed similar patterns to that observed when sucrose was supplied to the cells, except that an increase in dry mass was observed at the beginning of the stationary growth phase in the media containing only one monosaccharide. The increase in hexose levels in the media during the early stages of the cultures indicated extracellular hydrolysis of sucrose, which was further supported by the increase in the activity of acid invertase bound to the cell wall. Glucose was preferentially taken up, whereas uptake of fructose was delayed until glucose was nearly depleted from the medium. Measurements of intracellular sucrose content and cytoplasmatic and vacuolar invertases indicate that the enzymatic activity seems to be correlated with a decrease in the hexose flux into the cells of R. jasminoides. Our results indicate that the behavior of cell suspension cultures of R. jasminoides regarding sugar utilization seems to be similar to other dicotyledonous undifferentiated cell suspension cultures.

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On carbohydrate metabolism of cultured carrot root explants

Cited by 7 publications

References 8 publications

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR

Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

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On carbohydrate metabolism of cultured carrot root explants

Cited by 7 publications

References 8 publications

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Sucrose uptake and metabolism in a double layer system for micropropagation ofRosa multiflora

Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by 13C NMR

Differential sugar uptake by cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae

Contact Info

Product

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Substrate Utilization by Suspension Cultures and Somatic Embryos of Daucus carota L. Measured by ¹³C NMR