On a Substance Crossreacting with Luteinizing Hormone in the Pre-implantation Blastocyst Fluid of the Rabbit

Fujimoto, Seiichiro; Euker, Jean S.; Riegle, Gail D.; Dukelow, W. Richard

doi:10.2183/pjab1945.51.123

Cited by 18 publications

(7 citation statements)

References 9 publications

(1 reference statement)

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“…These observations support the concept that there may be a gonadotrophin in the preimplantation rabbit blastocyst and confirm and extend the earlier observations of and Fujimoto et al (1975). The inability of Sundaram et al (1975) to observe any activity in testis cells may have been due to the method of harvest, because freezing the entire blastocyst before separating the fluid may release proteolytic enzymes, or the gonadotrophin may be labile in the testis assay system.…”

Section: Discussionsupporting

confidence: 87%

“…observed that preimplantation rabbit blastocyst fluid contained a substance(s) which could inhibit the binding of labelled hCG to mem¬ branes of bovine luteal cells, and concluded that the blastocyst could secrete an hCG-like gonadotrophin which could act locally to stimulate luteal progestagen secretion before implantation. Fujimoto, Euker, Riegel & Dukelow (1975), using a non-specific LH antiserum, were also able to demonstrate a substance in blastocyst fluid which could inhibit binding of iodinated LH to the antiserum. However, Holt, Heise, Wilson & Keyes (1976) and Sundaram, Connell & Passantino (1975) were unable to detect any hCG-like activity in rabbit blastocyst fluid when using in-vitro assays with rat ovarian and Leydig cells, respectively.…”

Section: Introductionmentioning

confidence: 94%

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A stimulatory effect of the fluid from preimplantation rabbit blastocysts upon luteinization of monkey granulosa cell cultures

Cp¹,

Sl²,

Cn³

et al. 1978

Reproduction

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Summary. Blastocyst fluid was aspirated from Day 6\ m=1/ 2\ \ p=n-\ 7rabbit blastocysts and was added to cultures of granulosa cells obtained from preovulatory follicles of untreated rhesus monkeys or from follicles of monkeys treated with PMSG. The stimulation of progesterone secretion was measured and equated with that produced by hCG. The hCG-like activity was also measured in a radioreceptor assay using 125I-labelled hCG and porcine granulosa cells.In 8 out of 10 experiments with cultured cells from untreated monkeys, addition of 20% blastocyst fluid from Days 6\p=n-\9of culture stimulated progesterone secretion by 2\ x =r eq-\ to 6-fold. Similar findings were obtained in 5 experiments with cultures from PMSG\x=req-\ treated monkeys except that the blastocyst fluid was added from Days 0 to 6 of culture. The granulosa cells in such cultures underwent morphological luteinization. Compared to a standard of purified hCG the blastocyst fluid contained about 0\m=.\76\p=n-\2\m=.\5 ng hCG\x=req-\ like activity/ml which was non-dialysable. The radioreceptor assay indicated the presence of 0\ m=. \ 5\ p=n-\ 2\ m=. \ 5 ng hCG-like material/ml.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 94%

A stimulatory effect of the fluid from preimplantation rabbit blastocysts upon luteinization of monkey granulosa cell cultures

Cp¹,

Sl²,

Cn³

et al. 1978

Reproduction

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“…gonadotrophins (Fuchs and Beling 1974;Fujimoto et al 1975) or the ovine trophoblast protein-1 (Godkin et al 1984;Salamonsen et al 1988), which was recently characterized as embryonic interferon-~ (Imakawa et al I987; Roberts et al 1989). In rabbits comparable signal molecules have not yet been identified.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure, protein synthesis and secretion of day-6 rabbit blastocysts cultured in a chemically defined, protein-free medium

et al. 1990

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Day-6 rabbit blastocysts were cultured in Ham's F10 medium supplemented with polyvinylpyrrolidone as a macromolecular component, for 4 to 12 h. The integrity of the blastocyst cells was demonstrated by electron microscopy. Expansion and biosynthesis of proteins and of DNA were studied after culturing in the presence of 35S-methionine and 3H-thymidine. Polyvinylpyrrolidone did not interfere with the subsequent protein analysis, which was performed by two dimensional gel electrophoresis followed by silver staining and fluorography. More than 600 labelled proteins were found in the blastocyst tissue, many of them were also present in the blastocyst fluid and in the blastocyst coverings. Several proteins seemed to be produced for incorporation into the blastocyst coverings; others, only detected in the culture medium, might have been synthesized for secretion into the environment.

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“…Human chorionic gonadotrophin (hCG)-like acti¬ vity has been demonstrated in the blastocysts of mice (Wiley 1974;Sengupta et al 1978) and rabbits (Asch et al 1979; Varma et al 1979), employing immunohistochemical techniques and has also been quantitated by radioimmunoassay (Fujimoto et al 1975) and radioreceptor assays (Haour & Saxena 1974). Furthermore, the fluid from rabbit blastocyst has been reported to cause luteinization of granulosa cells from ovarian follicles of monkey, in vitro (Channing et al 1978).…”

mentioning

confidence: 99%

Effects of intrauterine instillation of antiserum to hCG during early pregnancy in mice

Joshi

Nandedkar

1984

Acta Endocrinologica

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Abstract. Human chorionic gonadotrophin (hCG)-like activity has been reported in mouse and rabbit blastocysts. The presence of this hCG-like activity seems to be essential for nidation of the pre-implantation embryo. Binding sites for hCG were localised on day 4 mouse embryos by immunohistochemical techniques. Presence of hCG-like activity was confirmed by cytotoxicity test. The number of implantation sites was significantly reduced on day 8 of pregnancy. Prior treatment to day 4 mouse embryos with hCG antiserum, for 1 h in utero or in vitro and their subsequent transfer to uteri of synchronised pseudopregnant mice resulted in impaired implantation of embryos, compared to controls treated with normal rabbit serum (NRS). These results suggest that hCG-like activity present on the pre-implantation embryo may have a significant role in implantation of the embryo.

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On a Substance Crossreacting with Luteinizing Hormone in the Pre-implantation Blastocyst Fluid of the Rabbit

Cited by 18 publications

References 9 publications

A stimulatory effect of the fluid from preimplantation rabbit blastocysts upon luteinization of monkey granulosa cell cultures

A stimulatory effect of the fluid from preimplantation rabbit blastocysts upon luteinization of monkey granulosa cell cultures

Ultrastructure, protein synthesis and secretion of day-6 rabbit blastocysts cultured in a chemically defined, protein-free medium

Effects of intrauterine instillation of antiserum to hCG during early pregnancy in mice

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