Ultrastructure, protein synthesis and secretion of day-6 rabbit blastocysts cultured in a chemically defined, protein-free medium

Henkel, Ralf; Dannhorn, D. R.; Petzoldt, Ulrich; Kirchner, C.

doi:10.1007/bf00178911

Cited by 8 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6) reflecting a possible glycoprotein. A similar set of proteins also has been described by Henkel et al (1990), although they were detectable in a more acidic area (4.6-5.4). This may be explained by the use of ampholines by Henkel et al (1990) instead of immobilines.…”

Section: Discussionmentioning

confidence: 71%

“…A similar set of proteins also has been described by Henkel et al (1990), although they were detectable in a more acidic area (4.6-5.4). This may be explained by the use of ampholines by Henkel et al (1990) instead of immobilines. This set of glycoproteins can be explained as transforming growth factor 'e', a large fragment of acrogranin (Parnell et al 1992;Diazo-Cueto et al 2000), which has been shown to run at 25 kDa (Parnell et al 1990(Parnell et al , 1992.…”

Section: Discussionmentioning

confidence: 71%

“…This explains why this protein is only detectable at day 5, although it is secreted by the endometrium from day 2 onwards (Beier 1978). Henkel et al (1990) described a similar spot to be uteroglobin within the extraembryonic matrices of day-6 rabbit embryos which we could show to be uteroglobin. The function of this protein is still under investigation, although immunological as well as carrier protein functions are discussed (Müller-Schöttle et al 2000).…”

Section: Discussionmentioning

confidence: 83%

See 2 more Smart Citations

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Herrler

Wolff²,

Beier

2002

Anatomy and Embryology

View full text Add to dashboard Cite

Preimplantation embryos of several species are surrounded by an extraembryonic matrix (often simply named zona pellucida) until briefly before implantation. All signals of the early embryo-maternal dialogue have to pass this matrix and therefore are detectable inside. We investigated the protein pattern of the extraembryonic matrices of 3-6-day-old rabbit embryos by two-dimensional gel-electrophoresis. Using (35)S-methionine incorporation, embryonic proteins were labelled and could be distinguished from maternal proteins. Furthermore, the presence of three different proteins (insulin-like growth factor-binding protein-3, uteroglobin, haptoglobin) within the matrices of day-6 embryos was investigated by Western blot analysis. The pattern and numbers of protein spots detected was clearly dependent on the time of embryonic development. Of all proteins detected, 19.3% and 33% are of embryonic origin (day 5 and day 6, respectively). At day 4 the zona proteins are no longer detectable, reflecting the degradation of the zona pellucida. From day 4 to day 5 proteins detectable within the extraembryonic matrices increase enormously. This demonstrates that embryo-maternal signalling accelerates at least 2 days before implantation. Insulin-like growth factor-binding protein-3, uteroglobin and haptoglobin are part of the early signalling as shown by Western blot analysis. Insulin-like growth factor-binding protein-3 could be detected as one spot at 38 kDa pI 6.1, uteroglobin at 8 kDa pI 6.0 and haptoglobin as two spots/isoforms at 36/38 kDa pI 5.8 and pI 6.0. These results demonstrate that extraembryonic matrices serving as a mailbox are a valuable tool for investigating early embryo-maternal signalling.

show abstract

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 83%

See 1 more Smart Citation

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Herrler

Wolff²,

Beier

2002

Anatomy and Embryology

View full text Add to dashboard Cite

show abstract

“…The suitability of this medium for the culture of rabbit blastocysts has previously been demonstrated (Henkel et al 1990). Addition of bFGF to the culture medium induced primitive streak formation in day 6 blastocysts within 24 h. This represents a slight acceleration in comparison to development in vivo, and could possibly be caused either by an overstimulation or by a premature stimulation of embryonic disc development in the culture system.…”

Section: Discussionmentioning

confidence: 96%

Fibroblast growth factor induces primitive streak formation in rabbit pre-implantation embryos in vitro

Angelis

Kirchner

1993

Anat Embryol

Self Cite

View full text Add to dashboard Cite

Culturing of rabbit pre-implantation embryos was performed in Ham's F10 medium supplemented with polyvinylpyrrolidone. Under these culture conditions, day 6 post coitum blastocysts increased their diameter within 24 h to 80% of that of day 7 blastocysts grown in vivo. Despite this substained growth, the embryonic disc remained undifferentiated with clear signs of degeneration after 24 h of culture. Basic fibroblast growth factor (bFGF) was able to overcome this developmental block. After 12 h of culture, day 6 blastocysts showed pear-shaped embryonic discs, and after 24 h, the primitive streak with Hensen's node was visible. The bFGF had no comparable effects on day 5 and day 7 blastocysts. The embryonic discs of day 5 blastocysts degenerated, even in the presence of bFGF, whereas day 7 blastocysts were able to form their primitive streak, also in the absence of bFGF. TGF beta 1 did not promote embryonic development in vitro. The data indicate that the onset of mesoderm formation in the rabbit is controlled by a growth factor of the FGF-family.

show abstract

“…On the other hand, the seven mentioned placental proteins of low molecular weight are clearly absent in the pre-implantation embryo (Henkel et al 1990;Hrab6 de Angelis and Kirchner 1993). Their nature has not yet been identified.…”

Section: Discussionmentioning

confidence: 99%

Placental lactogen-like proteins in the rabbit placenta

1993

Self Cite

View full text Add to dashboard Cite

Rabbit placentae and embryos at days 11 and 12 were analyzed by two-dimensional gel electrophoresis and by light microscopic histology for the presence of placental lactogen-like proteins. Immunoblotting and immunohistochemistry were performed by using a goat anti-human placental lactogen serum as well as a monoclonal mouse anti-human prolactin immunoglobulin; the results were similar. In the gel electrophoresis of placental tissue, three protein spots at pH 5.6 and 43, 39, and 35 kDa were immunostained; they were absent in the embryo. Immunoresponse was restricted to the cytotrophoblast. Immunofluorescent cells were mainly found on the proximal parts of the placental trabeculae.

show abstract

Ultrastructure, protein synthesis and secretion of day-6 rabbit blastocysts cultured in a chemically defined, protein-free medium

Cited by 8 publications

References 40 publications

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Proteins in the extraembryonic matrix of preimplantation rabbit embryos

Fibroblast growth factor induces primitive streak formation in rabbit pre-implantation embryos in vitro

Placental lactogen-like proteins in the rabbit placenta

Contact Info

Product

Resources

About