Omics-based approach in characterising mechanisms of entomopathogenic fungi pathogenicity: A case example of Beauveria bassiana

Harith-Fadzilah, Nazmi; Ghani, Idris Abd.; Hassan, Mohd Rohaizat

doi:10.1016/j.jksus.2020.101332

Cited by 15 publications

(14 citation statements)

References 55 publications

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“…After germinating, the conidia penetrate the host cuticle. The fungus then secretes various enzymes to counter the immune system of its host (Harith-Fadzilah et al 2021). Additionally, Chandler (2007) observed that the fungus also produces secondary metabolites such as oosporeins, tenellins, isarolides, beauverolides, and beauvericin which are toxic to the arthropod cells.…”

Section: Acaricidal Activity Of Sle On P Latus Is Comparable With Beauveria Spmentioning

confidence: 99%

Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia

2021

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Abstract. Puspitarini RD, Afandhi A, Fernando I. 2021. Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia. Biodiversitas 22: 2648-2655. The broad mite Polyphagotarsonemus latus (Banks) (Acari: Tarsonemidae) has been known to cause a great deal of injury on Jatropha curcas (L.) plantation in Indonesia. Isolation of indigenous fungal entomopathogens from rhizosphere soils, followed by pathogenicity assay was conducted to find an effective isolate for controlling P. latus. Additionally, the potential combination of the selected isolate and soursop (Annona muricata L.) aqueous leaf extract (SLE) was investigated. A total of 24 isolates were obtained by using the insect bait method. Four fungal isolates, namely Paecilomyces sp. 1., Lecanicillium sp., Beauveria sp., and Fusarium sp. 1, showed high conidial viability and were chosen as representatives to assess their pathogenicity against P. latus. Among the tested isolates, Beauveria sp. which had the highest conidial viability among the tested isolates, needed a shorter period to completely kill the tested mites. However, the compatibility test revealed the deleterious effect of SLE on Beauveria sp. SLE at all concentrations showed a very toxic effect on the fungus, therefore the two must be applied separately. Our results provide useful information on the effectiveness of indigenous entomopathogenic fungi Beauveria sp. and aqueous leaf extract of A. muricata as an alternative tool to control the broad mite P. latus on the J. curcas plantation.

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Section: Acaricidal Activity Of Sle On P Latus Is Comparable With Beauveria Spmentioning

confidence: 99%

Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia

2021

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“…Since the 1960s, entomopathogenic fungi (EPF) have been viewed as excellent biocontrol agents in the regulation of phytophagous insect and mite populations in agroecosystems (Li et al 2010;Nelly et al 2019;Khun et al 2020;Islam et al 2021). The pest control efficacy of various EPF species has been reported in Hypocreales (Ascomycota), but not limited to Beauveria (Mascarin and Jaronski 2016;Harith-Fadzilah et al 2021), Metarhizium (Aw and Hue 2017;Brunner-Mendoza et al 2019), and Paecilomyces (Moreno-gavíra et al 2020). As a result, numerous EPF species are already commercialized as mycopesticides worldwide (Goettel et al 2010;Dara et al 2019;Islam et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of soil-inhabiting entomopathogenic fungi within a conventional and organic farm and their virulence against Spodoptera litura

et al. 2022

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Abstract. Afandhi A, Choliq FA, Fernando I, Marpaung YMAN, Setiawan Y. 2022. Occurrence of soil-inhabiting entomopathogenic fungi within a conventional and organic farm and their virulence against Spodoptera litura. Biodiversitas 23: 1172-1180. Naturally occurring entomopathogenic fungi (EPF) are important components in agroecosystems as they serve as biocontrol agents of insect and mite pests. However, some cultivation practices may have deleterious effects on EPF. In this study, the occurrence of soil-inhabiting EPF was investigated between a conventional and organic farm. EPF was baited using Tenebrio molitor larvae, and their virulence was tested against Spodoptera litura larvae. The results showed a higher occurrence of EPF in the organic farm than the conventional farm, with Aspergillus sp., Beauveria sp., and Gliocladium sp. were exclusively found in organic soils. Among the twenty-five EPF isolates obtained, only four isolates were avirulent against S. litura larvae. Isolates belonging to Beauveria, Metarhizium, and Paecilomyces genera caused high mortality of S. litura larvae ranging from 40 to 65%. There was a significant positive relationship between the conidial viability of EPF and larval mortality. Since virulent isolates were found in conventional soils, efforts in preserving EPF prevalence are needed through the implementation of appropriate cultivation practices. The synthetic agrochemicals exclusion, organic fertilizers application, and crop rotation practiced in the organic farm should be integrated into any other agroecosystems as a form of conservation biological control strategies to strengthen the pest control service provided by EPF.

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“…The use of entomopathogenic fungi is still not familiar to farmers. Entomopathogenic fungi are able to become environmentally friendly bioinsecticides to control pests [6]. Entomopathogenic fungi have the potential as biological controllers of pests that are friendly to the environment.…”

Section: Introductionmentioning

confidence: 99%

“…This fungus can suppress pest populations such as pests that attack plant cultivation. Entomopathogenic fungi infect pests on plants by infecting the body of insects and entering the body through the skin, respiratory tract, digestive tract, or natural holes such as spiracles in insects [6]. Entomopathogenic fungi are host specific.…”

Section: Introductionmentioning

confidence: 99%

“…Entomopathogenic fungi have the potential to control pest populations. Entomopathogenic fungi are microorganisms that can be used as biological insecticides or bioinsecticides that can work to infect pests through several ways such as being eaten and damaging the digestive tract or penetrating insect cuticles [6]. Several entomopathogenic fungi have been effective in controlling pests on cultivated plants.…”

Section: Introductionmentioning

confidence: 99%

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Entomopathogenic Fungus from Rice Rhizosphere Soil in Politeknik Negeri Lampung Land

Budiarti

Nuryanti

2022

IOP Conf. Ser.: Earth Environ. Sci.

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The killing of non-target insects such as predators, natural enemies, and pest resistance to pesticides are negative impacts of pesticide application. Entomopathogenic fungi may be environmentally friendly biological controllers. This study aimed to conduct entomopathogenic fungi from rice plants in the Politeknik Negeri Lampung field using the insect bait method. Sampling was done by taking the rhizosphere soil of rice plants using the diagonal sampling method. Isolation and characterization of the morphology macroscopic and microscopic of entomopathogenic fungi were carried out at the Plant Laboratory of the Food Crops Production Study Program, Politeknik Negeri Lampung. The study started from June to November 2021. The stages of rearing T. molitor bait insects, fishing for entomopathogenic fungi, isolation and purification and propagation of entomopathogenic fungi, observing macroscopic and microscopic morphology. The research results from the exploration of entomopathogenic fungi in the rhizosphere of rice plants obtained that the fungus that infects the insect bait T. molitor with white, green, black hyphae and then microscopically observed the shape of the conidia and hyphae. Macroscopic and microscopic results showed that the entomopathogens obtained from insect bait using T. molitor larvae from the rhizosphere soil of rice plants were Aspergillus flavus, A. niger and Fusarium sp. Aspergillus spp. macroscopic, the mycelium of the fungus was green-white and black-white colours as for the mycelium Fusarium sp. white colour. The history of land use against insecticide applications can affect entomopathogens in rhizosphere soils.

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Omics-based approach in characterising mechanisms of entomopathogenic fungi pathogenicity: A case example of Beauveria bassiana

Cited by 15 publications

References 55 publications

Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia

Evaluation of indigenous fungal entomopathogens and aqueous leaf extract of Annona muricata against Polyphagotarsonemus latus infesting Jatropha curcas in Indonesia

Occurrence of soil-inhabiting entomopathogenic fungi within a conventional and organic farm and their virulence against Spodoptera litura

Entomopathogenic Fungus from Rice Rhizosphere Soil in Politeknik Negeri Lampung Land

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