Omics approaches for microalgal applications: Prospects and challenges

Mishra, Arti; Medhi, Kristina; Malaviya, Piyush; Thakur, Indu Shekhar

doi:10.1016/j.biortech.2019.121890

Cited by 92 publications

(22 citation statements)

References 118 publications

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“…They are one of the most promising candidates for various applications such as pharmaceuticals, bioenergy, industrial chemicals, nutraceuticals, and aquaculture [ 88 , 89 ]. To make most out of it, an axenic culture of diatom must be a prerequisite bearing in mind that a pure culture of diatoms is undoubtedly required in genome sequencing [ 90 ], to identify the producer of any novel bioactive compound for large scale manufacturing of nutraceutical [ 91 ], building a consortium for bioremediation [ 92 ] and to elucidate the relationship between other microalgae using omics tool [ 93 ]. Maintaining an axenic culture for a longer duration is very difficult because bacteria are vulnerable, which frequently attacks the diatom.…”

Section: Advanced Isolation Techniquesmentioning

confidence: 99%

Diatoms recovery from wastewater: Overview from an ecological and economic perspective

Saxena

Tiwari

Kaushik

et al. 2021

Journal of Water Process Engineering

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Section: Advanced Isolation Techniquesmentioning

confidence: 99%

Diatoms recovery from wastewater: Overview from an ecological and economic perspective

Saxena

Tiwari

Kaushik

et al. 2021

Journal of Water Process Engineering

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“…Several studies have performed an integrated metabolomics-proteomics, which could show a better landscape of the marine plankton [81,82]. Integrating "-omics" studies (e.g., genomics, proteomics, transcriptomics, metabolomics, lipidomics, and glycomics) will enable the investigation of the impacts of ecological interactions on organism physiology as well as evaluation of physiological and biochemical properties relating to human health for future applications [83,84].…”

Section: Discussionmentioning

confidence: 99%

Proteomic Profiling of Emiliania huxleyi Using a Three-Dimensional Separation Method Combined with Tandem Mass Spectrometry

et al. 2020

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Emiliania huxleyi is one of the most abundant marine planktons, and it has a crucial feature in the carbon cycle. However, proteomic analyses of Emiliania huxleyi have not been done extensively. In this study, a three-dimensional liquid chromatography (3D-LC) system consisting of strong cation exchange, high- and low-pH reversed-phase liquid chromatography was established for in-depth proteomic profiling of Emiliania huxleyi. From tryptic proteome digest, 70 fractions were generated and analyzed using liquid chromatography-tandem mass spectrometry. In total, more than 84,000 unique peptides and 10,000 proteins groups were identified with a false discovery rate of ≤0.01. The physicochemical properties of the identified peptides were evaluated. Using ClueGO, approximately 700 gene ontology terms and 15 pathways were defined from the identified protein groups with p-value ≤0.05, covering a wide range of biological processes, cellular components, and molecular functions. Many biological processes associated with CO2 fixation, photosynthesis, biosynthesis, and metabolic process were identified. Various molecular functions relating to protein binding and enzyme activities were also found. The 3D-LC strategy is a powerful approach for comparative proteomic studies on Emiliania huxleyi to reveal changes in its protein level and related mechanism.

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“…Synthetic biology and metabolic engineering made great strides in constructing and optimizing metabolic pathways in model microbes [32,108]. These methodologies could be crucial for the exploitation of photoautotrophic/bacterial co-cultures, especially if the aim is to achieve a deeper knowledge of the molecular mechanisms underlying a consortium and a refinement of the features that could ensure a more stable and productive co-culture.…”

Section: Improve the Consortia By Omics Approachesmentioning

confidence: 99%

Photoautotrophs–Bacteria Co-Cultures: Advances, Challenges and Applications

et al. 2021

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Photosynthetic microorganisms are among the fundamental living organisms exploited for millennia in many industrial applications, including the food chain, thanks to their adaptable behavior and intrinsic proprieties. The great multipotency of these photoautotroph microorganisms has been described through their attitude to become biofarm for the production of value-added compounds to develop functional foods and personalized drugs. Furthermore, such biological systems demonstrated their potential for green energy production (e.g., biofuel and green nanomaterials). In particular, the exploitation of photoautotrophs represents a concrete biorefinery system toward sustainability, currently a highly sought-after concept at the industrial level and for the environmental protection. However, technical and economic issues have been highlighted in the literature, and in particular, challenges and limitations have been identified. In this context, a new perspective has been recently considered to offer solutions and advances for the biomanufacturing of photosynthetic materials: the co-culture of photoautotrophs and bacteria. The rational of this review is to describe the recently released information regarding this microbial consortium, analyzing the critical issues, the strengths and the next challenges to be faced for the intentions attainment.

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Omics approaches for microalgal applications: Prospects and challenges

Cited by 92 publications

References 118 publications

Diatoms recovery from wastewater: Overview from an ecological and economic perspective

Diatoms recovery from wastewater: Overview from an ecological and economic perspective

Proteomic Profiling of Emiliania huxleyi Using a Three-Dimensional Separation Method Combined with Tandem Mass Spectrometry

Photoautotrophs–Bacteria Co-Cultures: Advances, Challenges and Applications

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