Olive oil hydroxytyrosol reduces toxicity evoked by acrylamide in human Caco-2 cells by preventing oxidative stress

Abstract

Humans are exposed to dietary acrylamide (AA) during their lifetime, it is therefore necessary to investigate the mechanisms associated with AA-induced toxic effects. Accumulating evidence indicates that oxidative stress contributes to AA cytotoxicity, thus, dietary antioxidants might have a protective role in colonic cells against AA toxicity. We have recently reported that hydroxytyrosol (HTy), a natural antioxidant abundant in olive oil, is able to enhance the cellular antioxidant defence capacity, thereby protecting cells from oxidative stress. In this study, we evaluate the protective role of HTy on alterations of the redox balance induced by AA in Caco-2 intestinal cells. AA cytotoxicity was counteracted by HTy by powerfully reducing ROS generation, recovering the excited enzyme antioxidant defences and decreasing phospho-Jun Kinase concentration and caspase-3 activity induced by AA. Therefore, AA-induced cytotoxicity and apoptosis are closely related to oxidative stress in Caco-2 cells and the olive oil natural dietary antioxidant HTy was able to contain AA toxicity by improving the redox status of Caco-2 cells and by partly restraining the apoptotic pathway activated by AA. Manuscript significanceThe results of the present study show that acrylamide-induced cytotoxicity and apoptosis are closely related to oxidative stress in Caco-2 cells and the olive oil natural dietary antioxidant HTy is able to decrease acrylamide toxicity by improving the cellular redox status and by partially restraining the apoptotic pathway activated by the toxin. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 Abstract Humans are exposed to dietary acrylamide (AA) during their lifetime, it is therefore necessary to investigate the mechanisms associated with AA-induced toxic effects. List of at least 4 recommended reviewers for the manuscriptAccumulating evidence indicates that oxidative stress contributes to AA cytotoxicity, thus, dietary antioxidants might have a protective role in colonic cells against AA toxicity. We have recently reported that hydroxytyrosol (HTy), a natural antioxidant abundant in olive oil, is able to enhance the cellular antioxidant defence capacity, thereby protecting cells from oxidative stress.In this study, we evaluate the protective role of HTy on alterations of the redox balance induced by AA in Caco-2 intestinal cells. AA cytotoxicity was counteracted by HTy by powerfully reducing ROS generation, recovering the excited enzyme antioxidant defences and decreasing phospho-Jun Kinase concentration and caspase-3 activity induced by AA. Therefore, AA-...

Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats

“…Furthermore, a significant increase in the MPO which removes H 2 O 2 and catalyzes the formation of toxic hypochlorous acid that leads to neutrophil infiltration and oxidative damage has been reported by Mustafa et al [13]. The present data is consistent with the previous study reporting that in the hepatic Caco-2 intestinal cells, AA induced oxidative stress through depletion of GSH, overproduction of ROS, and induction of apoptosis biomarkers that resulted in cell death [14].…”

The Effect of Subacute Exposure to Acrylamide on Femoral Bone Microstructure in Laboratory Mice

“…A few resorption lacunae were identified in these mice in pars anterior of the periosteal border. According to several authors [8][9]15] AA increases the oxidative stress and peroxidation of the lipids. The oxidative stress causes an increased production of reactive oxygen species (ROS) [16], which enhance osteoclastic activity [17] and bone resorption [18].…”

“…In human erythrocytes (in vitro), AA increases hemolysis and lipid peroxidation [8], suggesting that this neurotoxin affects redox status in cells [9]. The reported oral LD50 value is 107 mg/kg body weight (b.w.)…”

Inhibition of Neurosphere Formation in Neural Stem/Progenitor Cells by Acrylamide

“…The average daily intake of acrylamide for adults is approximately 0.5 mg/kg body weight; children may have two to three times more intake than adults (18,38,55 exposed people at a lifelong intake of 0.08 mg/kg body weight/day (47). Rodriguez-Ramiro et al have shown that smaller doses of acrylamide can act as toxicant when administered in long-term exposure (48). Therefore, it cannot be ruled out that daily exposure to a lower dose of acrylamide may bring forth a risk factor of toxicity in humans.…”